BACKGROUND:Studies have shown that overexpression of myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) is the main reason for early neuronal regeneration failure. OBJECTIVE:To study the effect of human amniotic mesenchymal stem cel s (hAMSCs) transplantation on neural functional recovery of rats with ischemia/reperfusion (I/R) injury. METHODS:Sixty Sprague-Dawley were randomized into sham, I/R, and hAMSCs groups (n=20 per group). Rats in the hAMSCs group were given 1 mL of hAMSCs suspension (1.0×108/L) via the tail vein 24 hours after I/R injury. Rat neurological function recovery was assessed based on behavior changes, as determined by Longa behavioral score, cylinder test, horizontal ladder walking test and limb symmetry test at 1, 3 days, and 1, 2, 3 weeks post transplantation. Cel migration and distribution were observed using immunofluorescence method at 1 day, and 1, 2, 3 weeks post transplantation. MAG and OMgp protein expression was detected by western blot assay at 2 weeks post transplantation. Neuronal apoptosis was detected by TUNEL staining at 3 weeks post transplantation. RESULTS AND CONCLUSION:In the hAMSCs transplantation group, red marker-positive cel s were visible around the injury region at 1 week after transplantation, and over time, these cel s were increased in number. Significant improvement in the neurological function of rats were observed in the hAMSCs group as compared with the I/R group at 3 days and 1, 2, 3 weeks after transplantation (P<0.05), and the expression of MAG and OMgp proteins were also decreased dramatical y in the hAMSCs group (P<0.05). After I/R injury, the number of apoptotic cel s was increased, but hAMSCs transplantation reversed this effect. Overal , hAMSCs transplantation can reduce neuronal apoptosis by reducing MAG and OMgp expression levels, and thereby promote neurological functional recovery from I/R injury in rats.

Aromatic hydrocarbons are capital environmental contaminations. Pseudomonas alcaligenes NCIB 9867 (P25X) is capable of degrading aromatic hydrocarbons via the gentisate pathway. Biochemical characterization indicated that it has isofunctional enzymes for the mono- and dioxygenase- catalysed reactions. One set of the enzymes is constitutive and the other is strictly inducible. To date, only the genes for the constitutively-expressed gentisate dioxygenase and the downstream enzymes have been cloned. A mutant strain of P25X, designated SNZ28, which had the constitutive copy of the gentisate 1, 2-dioxygenase gene interrupted by a streptomycin/spectinomycin resistance gene cassette, was found to still express gentisate dioxygenase when induced by gentisate. The proteome profiles of P25X and mutant SNZ28, grown in the presence and absence of the aromatic inducer gentisate, were compared after 2D-PAGE. Fifteen distinctive protein spots which were observed only in induced cells of P25X and mutant SNZ28 but absent in non-induced cells of both were further analyzed by MALDI-TOF and Q-TOF. Of the 15 proteins, 12 showed significant sequence similarity to proteins with assigned function in other microorganisms . The identification of protein P4 which showed positive identification to a gentisate dioxygenase from Ralstonia species indicated the putative role of this protein to encode gentisate 1, 2-dioxygenase in P.alcaligenes.

OBJECTIVE: To summarize the approaches and methods for clinical pharmacists to provide clinical pharmaceutical care.METHODS: By reviewing pertinent literature combining the working experience of the clinical pharmacists,the approaches and methods for clinical pharmacists to provide pharmaceutical care were explored.RESULTS: Pharmacists could provide clinical pharmaceutical care by participating in clinical rounds,consultation and pharmaceutical monitoring,intervening medical order,offering drug-counseling service,handling the adverse clinical pharmacy events and providing medication education etc.CONCLUSIONS: Clinical pharmacists can provide various kinds of clinical pharmaceutical services for the safe,effective and economical use of drugs.

OBJECTIVE:To investigate the status quo and rationality of the perioperative use of drugs in patients with cataracta. METHODS:The drug use data of 60 patients undergoing cataracta surgery in our hospital from Apr. 2007 to Mar. 2008 were analyzed retrospectively and statistically. RESULTS:The drugs used during perioperative period consisted of antibacterials (applied either systemically or locally) and other assistant drugs (blood coagulants,drugs for decreasing intraocular pressure,anti inflammatory drugs and Chinese patent medicines). All the patients were treated with systematic antibacterials for prevention medication. The systemically used antibacterials consisted of ?-lactam antibiotics,clindamycins,aminoglycosides,fluoroquinolones and amphemycins. The problems existed in systemic use of the antibacterials manifested as irrationality in the choice of drug category,dosage,administration frequency,administration time and drug combination,etc. The locally applied antibacterials consisted of aminoglycosides,fluoroquinolones,tetracyclines and lincomycins. The problems existed in the local application of the antibacterials manifested as irrationality in administration time,drug change and drug combination,etc. CONCLUSION:The rationality of the perioperative drug use in patients with cataracta in our hospital remains to be improved.

OBJECTIVE:To analyze the problems existing in the use of thiamphenicol for outpatients. METHODS:In this retrospective study,a total of 743 outpatient prescriptions of Thiamphenicol enteric-coated tablets in one hospital during Mar. 2008 were analyzed in respect of the prescription writing,medication suitability and amount of money of prescriptions,etc. RESULTS:The problems in the use of Thiamphenicol manifested as nonstandard in prescription writing,irrational in clinical drug choice,drug combination,administration and dosage,etc. CONCLUSION:The use of Thiamphenicol in the hospital is somewhat irrational thus remains to be standardized further.

Lactoferrin(LF) belongs to the transferrin family and is a multifunctional glycoprotein with a variety of biological activities, which not only binds to viral antigenic determinants to inhibit the recognition and binding of viruses to host cells,but also inhibits cell carcinogenesis through the production of thiobarbituric acid, malondialdehyde and other substances with strong antioxidant properties. In recent years, it has been found that in addition to its antiviral and anti-inflammatory functions, LF also has the ability to promote the repair of various types of damaged tissues. This paper mainly reviews the repairing and pro-repairing effects of LF after skin, muscle, bone, intestinal, brain and gingival injuries, aiming to elucidate the further application prospects of LF in the medical field.

High myopia is frequently associated with retinal degenerative changes (such as myopic foveoschisis, macular hole and its related retinal detachment, and choroidal neovascularization) which are the major causes of visual loss in high myopic eyes. Optical coherence tomography may help us to understand the macular lesions in high myopia. Peeling of internal limiting membrane and removal of posterior vitreous cortex at an appropriate time, are believed to facilitate restoring the retinal flexibility and resolution of myopic foveoschisis, and promote the macular hole closure and retinal reattachment. Anti-angiogenesis treatment combined with photodynamic therapy and (or) corticosteroid therapy are the future options to treat myopic choroidal neovascularization. Correctly understanding and mastering the methods and timing of diagnosis and treatment of high myopia-related macular degeneration, and taking targeted interventions to enable patients to be rational and effective treated, are the keys to further reduce the damage of visual function in patients with high myopia.

China ; Humans ; Pancreatitis, Acute Necrotizing ; diagnosis ; therapy ; Practice Guidelines as Topic

Adrenergic beta-2 Receptor Agonists ; administration & dosage ; therapeutic use ; Asthma ; diagnosis ; etiology ; therapy ; Child ; Child, Preschool ; Chronic Disease ; Glucocorticoids ; administration & dosage ; therapeutic use ; Humans ; Infant ; Pediatrics ; Seasons ; Time Factors

Biomedical Research ; methods ; trends ; China ; Combined Modality Therapy ; Humans ; Management Information Systems ; trends ; Multicenter Studies as Topic ; Pancreatic Neoplasms ; therapy