Objective: To investigate effects of glucose and free fatty acid at different concentrations on phosphorylation of adenosine-5′-monophosphate activated protein kinase(AMPK) and acetyl CoA carboxylase(ACC) in INS-1 cells,and effects of globular adiponectin on phosphorylation of AMPK and ACC.Methods: INS-1 cells were cultured and treated with 5 mmol/L glucose or 0.25 mmol/L free fatty acids,and time courses and dose responses of different dosages of glucose and fatty acid on phosphorylation of AMPK and ACC were measured.We measured the effects of the pharmacological AMPK activator AICAR(5-aminoimidazole-4-carboxamide-riboside) and globular adiponectin on phosphorylation of AMPK and ACC.Results: Glucose and fatty acid at different concentrations inhibited the phosphorylation of AMPK and ACC at the end of 60 min,but AICAR increased the phosphorylation of AMPK and ACC significantly,while 2.5 mg/L globular adiponectin increased the phosphorylation of AMPK and ACC by 23%(P

Female rats were fed with lead acetate 100mg/kg/day(group Ⅰ) or 200 mg/kg/day (group Ⅱ) for 5 days, at the day 7 to 11 of gestation prior to killing. The results shown that the mean level of blood lead in rats of group I was 27.90?8.47?g/dl, and corpus luteum or placenta were injured lightly. In the group Ⅱ, the level of blood lead reached 48.77?16.15?g/dl which correspond to human subacute intoxication, the structure of corpus luteum and placenta were injured prominently, and the activity of 3-hydroxysteroid dehydrogenase is lower than that of control group, about 1/20 of latter. The number of embryos in group Ⅱ were absorbed about 31.69%. However, the gonadotropic cells of pituitary did not affected prominently after treatment with lead. The human chorionic villi of placenta were cultured in medium with lead acetate 0.51?g/ml, 2.5?g/ml, 5?g/ml respectively, for 3 to 4 days. The struture of chorionic villi were damaged obviously after culture with lead more than 2.51?g/ml, and the concentration of hCG in culture medium was decreased. The results indicated that lead affected the corpus luteum, placenta and embryo, and exerted direct action on human trophoblast.

Objective To investigate the natural rotation of the fetal head during labor when it was engaged by occiput transverse (OT) or occiput posterior(OP) positions.Methods A total of 1 013 parturients was included who had a singleton term gestation, vertex presentation, OT or OP position with cervical dilatation of ≤ 3 cm, and vaginal delivery.All parturients were delivered in Xiamen Hospital of Traditional Chinese Medicine during April 1 to December 31,2014.Cervical dilation, station, and fetal position of every delivery were systematically recorded.Main outcome was the cervical dilation and station of anterior rotation from OT or OP position.They were Stratified by cervical dilation every 2 cm since 4 cm into 4 ～ 5 cm, 6 ～7 cm, 8 ～9 cm, 10 cm;or were stratified by fetal head station every 1 cm since-2 (S-2) into S-1 , S0, S+1 , S+2 , S+3.The differences in anterior rotation rate, dilatation, and head station between OT and OP were analyzed.Results There were 850 OT and 407 OP with cervical dilatation of ≤≤ 3 cm.The rate of anterior rotation was 82.5 ％ (701/850) and 76.7 ％ (312/407), respectively.The constituent ratio of OT rotating into occiput anterior position (OA) at different dilatation was different with that of OP (x2 =260.93, P ＜ 0.01).The constituent ratio of OT rotating into OA at different fetal head station was different with that of OP (x2 =133.18, P ＜0.01).The cervical dilatation and fetal head station that make it easy for OT 's anterior rotation were 6 ～7 cm (OR =5.81 , 95％ CI :3.81 ～8.87), 8 ～9 cm(OR =4.99 ,95％ CI:3.19 ～7.83), and S+1(OR =3.01, 95％ CI: 1.99 ～4.54), S+2(OR =5.43, 95％ CI:3.53 ～ 8.35).The cervical dilatation and fetal head station that make it easy for OP 's anterior rotation were 6～7 cm(OR =4.11,95％ CI :2.24 ～7.89), 8 ～9 cm(OR =27.31, 95％ CI :15.12 ～49.34), 10 cm(OR =27.76 , 95％ CI :14.87 ～51.82), S+2(OR =4.84 , 95％ CI :3.03 ～7.73).Conclusions The anterior rotation time of OT is different from OP.It's easy for OT to rotate into OA when cervix dilate into 6 ～9 cm and fetal head station is at S+1 ～ S+2.When cervix dilate into 6 ～ 10 cm and fetal head station is at S+2, it's easy for OP to rotate into OA.

Objective To observe the effect of modified Fu Qingzhu's Shenghua decoction on uterine involution after repeat cesarean section.Methods Randomized and parallel design was adopted in the study.A total of 112 cases were recruited into an observation group and a control group.56 cases in the observation group were treated with oral Fu Qingzhu's Shenghua decoction for 5 days,while 56 cases in control group were treated without any herbs.The follow-up was 42 days.Duration of lochia,hemoglobin concentration,and abnormalities of uterine ultrasound were observed.Results Duration of lochia rubra (5.1 ± 1.2 d vs.5.8 ± 1.3 d;t=-2.734,P=0.007) and the duration of lochia rubra and lochia serosa in the observation group 25.0 (21.0,29.8) d vs.29.0 (26.0,33.8) d;Z=3.873,P＜0.001) were all lower than the control group.The lochia complete ration in 42 days (94.9％ vs.82.1％,x2=4.264),the average hemoglobin concentration (115.43 ± 10.68 g/L vs.104.29 ± 9.90 g/L,F=4.159) and sum of the three uterine diameters (14.2 ± 1.0 cm vs.14.8 ± 1.3 cm,t=-2.686,P=0.008) in the observation group were all higher than the control group.The incidence of uterine cavity hydrops (3.57％ vs.17.85％,P=0.015) in the observation group was lower than the control group.Conclusion Fu Qingzhu's Shenghua decoction has promotive effects on uterine involution after repeat cesarean section.

Venous valve plays an important role in regulating the venous flow,especially in preventing the blood backflow.It has heen observed that when deep vein thrombosis and dissolved,the valves in the lesions had been destroied or dysfunction.The mechanism of damage of vein valve has not been fully clarified,but as we know,it's common that the inflammatory reaction,due to many unfavorable factors,can cause thrombosis.On the other hand,deep vein thrombosis could bring out the inflammatory reaction as well.So the author will review the development of researching of the destruction of venous valves in recent years.

[Objective] To observe the therapeutic effect of puerain with corticosteroid on nonanterior ischmic optic neuropathy (NAION).[Methods] In 58 patients(60 eyes)who were diagnosed as NA10N,29 patients (30 eyes)underwent intravenous drip with puerain and corticosteroid (treatment group);and the other 29 patients (3o eyes)underwent intravenous drip with corticosteroid and compound anisondine (control group).[Results] The visual acuity was better in treatment group than that in control group with a significant difference (x2=4.83,P

OBJECTIVESTo investigate whether tibial intercondylar hyperplasia can cause anterior cruciate ligament (ACL) injuries.

METHODSFrom February 2009 to October 2012, 58 cases (including 70 knees) with knee osteoarthritis (KOA) without trauma history were selected from the out-patient department of orthopaedics in Guang'anmen Hospital attached to China Academy of Chinese Medical Science. All cases were tested by MRI scanning, then vertical height of the medial and lateral intercondylar spine in coronal position and the area of abnormal signal of ACL in sagittal position were tested from patients. Correlation between the height of intercondylar spine and the area of abnormal signal of ACL were analyzed.

RESULTSFives cases of 58 cases( 70 knees) were excluded from episome in knee joint, and other 65 knees were in accordance with the inclusion criteria. Among them, there were 10 males and 48 females aged from 39 to 79 years old with an average of 61 years old. The vertical height of the medial intercondylar spine was (10.02 +/- 1.46) mm and lateral was (8.92 +/- 1.69) mm. The area of abnormal signal ACL was (318.42 +/- 130.10) mm2. There was positive correlation between the height of medial intercondylar spine and the area of abnormal signal of ACL (r=0.06, P=0.00). But there was no correlation between lateral intercondylar spine and the area of abnormal signal of ACL (P=0.10).

CONCLUSIONThe height of medial intercondylar spine and the area of abnormal signal of ACL is positively correlated. The clinical significance is in study, while the clinical meaning still need further study.

Adult ; Aged ; Anterior Cruciate Ligament ; abnormalities ; diagnostic imaging ; surgery ; Female ; Humans ; Hyperplasia ; diagnostic imaging ; surgery ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Osteoarthritis, Knee ; diagnostic imaging ; surgery ; Radiography ; Tibia ; diagnostic imaging ; pathology ; surgery

Objective To study the effects of PNESF at different voltages on the life spans of ascites tumor bearing mice.Methods Forty ascites tumor bearing mice were divided into 5 groups and exposed to PNESF at 0,10,17,23 and 30 kV,respectively,for 30 minutes daily on 4 successive days.All of the animals then received routine care until death.Their eating habits,body weights and life spans were observed.A straight line fitting method was used for statistical analysis. Results The body weights of the mice exposed to 17 kV and 23 kV PNESF increased quickly during the four days of PNESF exposure.The weight gain in all groups declined after PNESF exposure was stopped.Thereafter the body weights of the mice exposed to the 17 kV PNESF increased more slowly than those in the control group.The mice exposed to the 23 kV PNESF gained weight more quickly than other groups.The mice exposed to the 17 kV PNESF lived the longest,demonstrating a lengthening of life of 25.5%compared with the control group.Conclusion Exposure to a PNESF of appropriate intensity might significantly increase the life span of ascites tumor bearing mice.

Imaging techniques play a vital role in evaluating myocardial damage in patients with myocardial infarction. Accurate evaluations of postinfarction function and scar can help identify high-risk patients and provide prognosis information, which contributes much to clinical practice. The assessment of myocardial damage mainly includes overall evaluations of ventricular function and remodeling and targeted characterization of myocardial infarction including infarct size, myocardial viability and microvascular obstruction. Echocardiography, cardiac magnetic resonance, CT and nuclear examinations are most common imaging techniques currently. This review is to update evidence on applications of these modalities in evaluation of postinfarction myocardial damage and offer some helps to health workers.

ObjectiveTo investigate the relationship between enhanced CT examination and acute kidney injury in patients with acute pancreatitis.MethodsThe clinical data of 162 acute pancreatitis patients were analyzed retrospectively.Acute kidney injury was diagnosed and graded by RIFLE criterion.The patients were divided into two groups according to enhanced CT examination:enhanced CT group and non-enhanced CT group.ResultsEnhanced CT group had 85 cases,and non-enhanced CT group had 77 cases.The increased creatinine level in enhanced CT group was higher than that in non-enhanced CT group [(59 ± 13 ) μ mol/L vs.(38 ± 11 ) μ mol/L],and there was significant difference between two groups (P ＜0.01 ).The incidence of acute kidney injury in enhanced CT group was higher than that in non-enhanced CT group [30.6％ (26/85) vs.15.6％ (12/77)],and there was significant difference between two groups (P ＜0.01 ).Enhanced CT examination increased the risk of acute kidney injury occurrence (OR =1.293,95％ CI1.089-1.443).After adjusted by diabetes and APACHE Ⅱ score,enhanced CT examination was still anindependent risk factor of acute kidney injury (OR =1.282,95％ CI 1.185-1.377 ).ConclusionsEnhanced CT examination is a potential risk factor of acute kidney injury in acute pancreatitis patients.The time of enhanced CT examination is worthy of further study.