In university research institutions,when in charge of several projects at the same time,the project leader will inevitably pay not enough attention to every project.In order to ensure the progress and quality of each research project,the study designs and builds project managercentered management mechanisms,redesigns the research and development structure from the system perspective,establishes the crossbar management model,and further analyzes the internal communication mechanism,the power-and-responsibility division mechanism,and oversight mechanism.

Objective: To evaluate the clinical efficacy and safety of ticagrelor in the patients with acute coronary syndrome ( ACS) . Methods:A retrospective study was applied to investigate the ACS patients treated with ticagrelor in our hospital from July to December in 2015. The basic information of patients, drug administration, platelet aggregation induced by ADP, major adverse cardio-vascular events ( cardiac death,nonfatal myocardial infarction,target vessel revascularization and stent thrombosis) , and adverse drug reactions ( ADR) were recorded. The incidence of end point events was calculated and the change of platelet aggregation induced by ADP before and after the drug administration was analyzed by SPSS statistical software. Results:A total of 161 patients were collected. The incidence of cardiovascular adverse events was 1. 2%, while the incidence of adverse drug reactions was 30. 4% including bleeding (15. 5%) without severe bleeding events and dyspnea (10. 6%) with 3 severe ones. The platelet aggregation rate before and after the ticagrelor treatment respectively was (54. 96 ± 14. 654)%and(24. 37 ± 13. 183)% in 122 patients with low reaction to clopidogrel( P<0. 01). Conclusion:Ticagrelor at the recommended dose can further reduce the platelet aggregation induced by ADP. In spite of high incidence of ADR, ticagrelor has slight ADR with good tolerance.

Objective To analyze the clinical feature,pathogenesis and treatment of autoimmune hemolytic anemia (AIHA) in patients after orthotopic liver transplantation.Method A retrospective analysis was conducted on 7 patients who developed AHA after orthotopic liver transplantation.The clinical feature,pathogenesis and treatment of AIHA were reviewed.Result The incidence of AIHA was 0.98％ (7/713).AIHA occurred (7.9-± 4.0) (3-14) days after transplantation.All patients were characterized by progressive anemia and jaundice.Laboratory examination showed lower hemoglobin and platelet count,higher lactate dehydrogenase,higher indirect bilirubin and higher reticuloeyte count.The causes of AIHA included ABO-incompatible liver transplantation,infection and repeated transfusion.Conclusion AIHA is a rare and serious complication after liver transplantation.The most cause is ABO-incompatible liver transplantation.The patients can be successfully cured if the diagnosis is clarified and the treatment is definitive.

The respiratory tract is constantly exposed to environmental elements so that pathogens invade the lung easily and induce infectious disease.Most microbes could be eliminated by innate immunity and adaptive immunity.In healthy host,but in immunocompromised hosts such as HIV infection,transplant recipients,cancer,collagen vascular disease and critical illness,the lungs are more susceptible to bacteria,fungal and virus. Abstract:Summ ary: The resp iratory tract is constantly exposed to environm ental elem ents so that pathogens invade the lung easily and induce infectious d isease.Mostm icrobes cou ld be elim ina-ted by innate immun ity and adaptive immun ity.In healthy host,but in immunocomprom ised hosts such as H IV infection,trans-p lant rec ip ients,cancer,collagen vascu lar d isease and critical illness,the lungs are more susceptib le to bacteria,fungal and virus.

Objective To investigate the effect of histone deacetylases-1(HDAC-1)siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells.Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA.HDAC-1 protein was detected by Western blot.The cell growth inhibition was assesses by MTT and clone forming assay.Apoptosis was measured by flow cytometry.Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously.The down-regulation of HDAC-1 significantly reduced the colony formation rate,inhibited cell proliferation and induced apoptosis in HeLa cells.Conclusion HDAC-1 siRNA may play an anti-proliferation role in HeLa cells by inducing apoptosis.

Objective To evaluate the role of postoperative electron beam radiotherapy in the management of ke-loids. Methods Between January 2004 and December 2006,116 cases of keloids were treated with 6 MeV electron beam radiotherapy within 24 h after surgical resection of the keloids. All patients had received a total of 21 Gy in seven daily 3-Gy fractions. Treatment was started at the 24th h after surgery. Patients were followed up and the information regarding treatment results, early and late radiation toxicity,and the satisfaction level by self-assessments was recorded. Results Recurrence occurred in 17 cases of keloids(14. 7%),and was correlated with site of the lesions(χ~2 =29. 91,P<0. 01). Most recurrences were observed at site of sternum (10/43) and shoulder (5/13 ). Keloid associated symptoms, e. g. itching and pain, were improved in 88. %%. For early toxicity outcomes, 100% had grade 1～2 skin erythema,7. 8% had wound delayed union,and 4. 3% had infection. For late toxicity outcomes, 30. 2% reported grade 1～2 hyperpigmentation, 11. 2% grade 1～2 hypopigmentation, and 5. 2% grade 1 telangiectasia. No severe complications or secondary malignancies were observed. 72. 4% patients described the results of treatment as excellent or good,and 15. 5% patients were not satisfied with the treatment results. Conclusion Postoperative electron radiotherapy is well tolerated and effective in preventing keloid recurrence.

Objective:To establish an HPLC-FD method for determining the content of Angelica sinensis polysaccharide (ASP1) to lay foundation for its pharmacokinetic study in rats. Methods: Purified ASP1 was labeled with FITC by the method of Belder and Granath to obtain ASP1-FITC. The tissue samples were treated with 30% trichloroacetic acid and 11% NaOH before injection. The samples were determined by HPLC-FD. A PL aquagel-OH MIXED column was used,and the mobile phase was phosphate buffer( dis-solve NaH2PO32. 34g , Na2HPO34. 33 g and NaCl 11. 70 g into 1000 ml water) with pH of 7. 0. The flow rate was 0. 5 ml·min-1. The excitation and emission wavelengths was set at 495 nm and 520 nm, respectively. Results:The linear calibration curve was within the concentration range of 0. 25-40. 00 μg·ml-1(r=0. 9996)with the lower limit of quantification of 0. 20μg·ml-1 in tissue sam-ples. The extraction recovery of ASP1 was determined at low, medium and high concentration with the recovery of 91. 98%-114. 20%. The intra and inter-day RSDs were lower than 8. 31% and 2. 94%, respectively. Conclusion:The method to determine the content of ASP1 in rats by HPLC-FD with pre-column derivatization has been esablished. It is simple, accurate and reliable, which can be suc-cessfully applied in the study of pharmacokinetics and tissue distribution of ASP1 in rats.

Thirty cases of cerebral hemorrhage were treated by regular therapy plus hemotherapy with ultraviolet radiation as control, with another 30 such cases treated by the same method plus injection of Radix A. Senticosi. Results showed that the marked effect of treatment group was 96. 7% and total effective rate 100%. while that of the control group were 60. 0% and 83. 3% respectively, (P

Acute Disease ; Animals ; Dimethoate ; analogs & derivatives ; poisoning ; Disease Models, Animal ; Male ; Mice ; Muscle Weakness ; chemically induced ; pathology ; Muscles ; pathology

Objective To investigate the effects of propofol on mitochondrial membrane permeability during hypoxia/reoxygenation (H/R) in rat hippocampal neurons. Methods Primary cultured hippocampal neurons of fetal rats obtained from Wistar (17-18 days of gestation) were randomly divided into 3 groups: Ⅰ control group (group C), Ⅱ H/R group and Ⅲ propofol + H/R group. Neurons were cultured in the culture medium with combined oxygen glucose deprivation for 2 h followed by reoxygenation in group Ⅱ and Ⅲ. In group Ⅲ propofol was added to the culture medium with the final concentration of 20 μ mol/L before combined oxygen glucose deprivation.Neuronal viability was detected by MTT assay and mitochondrial membrane potential (MMP) with flow cytometry at 0, 4, 8, 12 and 24 h after reoxygenation (T1-5) and the permeability of cell membrane and mitochondrial membrane was monitored at T5 using laser confocal scanning microscope. Results The neuronal viability and MMP were significantly decreased (P ＜ 0.05), while the permeability of cell membrane and mitochondrial membrane was increased at T5 in group Ⅱ as compared to group Ⅰ . The neuronal viability at T1-4 and MMP at T1-5 were significantly increased (P ＜ 0.05), while the permeability of cell membrane and mitochondrial membrane was decreased at T5 in group Ⅲ compared to group Ⅱ . Conclusion Propofol can protect rat hippocampal neurons against H/R injury through increasing MMP, improving the cell and mitochondrial membrane permeability, and increasing the neuronal viability.