Rat liver mitochondria were exposed to various conentrations of halothane,enflurane, isoflurane and sevoflurane. Electron transfer rates from NADH and succinate to cytochrome C were measured by scanning dual wavelength spectrophotometer. Statistical analysis of the data suggested that halothane at clinical or higher than clinical concentrations markedly inhibited activities of NADH-Cyt,C reductase.in contrast,no decrease occurred in the activities of NADH dehydrogenase,NADH-coenzyme Q reductase and enzymatical system of succinate chain. Enflurane,isoflurane and sevoflurane had little effect on enzymatical system of mitochondrial electron transfer chain. These data indicate that halothane interfere with utilization of NADH-linked substrate by blocking electon transport from NADH to cytochrome C and it is probable that the locus of action is at Q binding protein(Qpn) or complex of Qpn and ubiquinone.

OBJECTIVE:To set up data management system for therapeutic drug monitoring(TDM),and to realize the remote database access by means of network.METHODS:The TDM data management system was set up by means of com?puter network technology and database technology.RESULTS&CONCLUSION:A satisfactory management interface and remote database access were available through TDM Data management system,which were sure to benefit clinic and patients.

BACKGROUND: For elderly patients with unstable intertrochanteric fractures, hip arthroplasty is mainly applied due to the degenerative body function and difficulty in reduction.OBJECTIVE: To investigate the effectiveness and safety of cemented versus cementless hip arthroplasty for senile unstable intertrochanteric fractures.METHODS: We are conducting a prospective, single-center, non-randomized, controlled clinical trial at the Harrison International Peace Hospital, China. Eighty-six elderly patients with unstable intertrochanteric fractures were enrolled and allotted to control (n=44) and experimental (n=42) groups, followed by treated with cemented and cementless hip arthroplasty, respectively. The follow-up time is 6 months. The primary outcome is good and excellent rate according to Harris hip scores at 6 months postoperatively. The secondary outcomes are Harris hip scores and hip morphology on X-ray at 1, 3 and 6 months postoperatively, intraoperative blood loss, operation time, blood transfusion volume,time of off-bed and drainage volume at 1 month postoperatively, as well as the safety based on the incidence of adverse events at 6 months postoperatively. Partial results show that the blood transfusion volume and drainage volume postoperatively in the experimental group were significaintly higher than those in the control group (P < 0.05).There were no significant differences in the good and excellent rate according to Harris hip scores, Harris hip scores and incidence of adverse events between two groups at 6 months postoperatively (P > 0.05). There were four cases of bone cement implantation syndrome in the control group. The study protocol has been approved by the Ethics Committee of Harrison International Peace Hospital of China, approval number 2013-37. All protocols will be performed in accordance with the Ethical Principles for Medical Research Involving Human Subjects in the Declaration of Helsinki. Written informed consent was provided by each patient and their family members after they indicated that they fully understood the treatment plan.DISCUSSION: Cases were collected in January 2014 and the trial will be finished in December 2017. Cemented and cementless hip arthroplasty are mainly used to treat senile unstable intertrochanteric fractures. This study is designed to investigate the differences between cemented and cementless hip arthroplasty, thus providing referece for the treatment of unstable intertrochanteric fractures in the elderly. Partial trial results indicate that both cemented and cementless hip arthroplasties exhibit good curative efficacy and safety for senile unstable intertrochanteric fractures, while the occurrence of bone cement implantation syndrome in cemented hip arthroplasty should never be neglected.

Objective:To design a mobile health service platform based on cloud computing so as to reform the current medical problems including serious waste of medical resources, low level of information sharing and poor feel of medical treatment and so on.Methods: On the basic of analyzing cloud computing and relative theories, a solution of mobile medical cloud based on Google App Engine(GAE) was proposed.Results: A mobile medical health service platform including overall business process and deployment of development environment based on GAE has been designed and the traditional medical service mode has been converted.Conclusion: The exploration and attempt that constructe mobile medical health service platform by combining cloud computing with medical services can promote the conversion of medical mode and provide a effective solution for meeting the requirements of medical service quality and service efficiency for the masses.

BacKground:Lymphocyte function-associated antigen-1( LFA-1 ) pIays a cruciaI roIe in the pathogenesis of infIammatory boweI disease by reguIating the activation and function of T ceIIs. Aims:To investigate the effect of LFA-1 deficiency( LFA-1-/-)on differentiation of mice na?ve T ceIIs into Th17 ceIIs in vitro. Methods:LFA-1-/- mice were breeded and the progeny genome DNA was extracted from the taiIs for genotyping by PCR. CD4+CD62L+ na?ve T ceIIs were separated from spIenic mononucIear ceIIs of LFA-1-/- progeny mice and wiId type ( WT ) C57BL/6J mice, respectiveIy,by magnetic-activated ceII sorting( MACS),and then the purity of separated ceIIs was determined. Na?ve T ceIIs obtained were cuItured in different inducing systems[ transforming growth factor-β( TGF-β),TGF-β + interIeukin-6 (IL-6),and TGF-β + IL-6 + IL-23]in vitro for Th17 ceII differentiation;ceIIs in each inducing system were coIIected for anaIyzing the ratio of Th17 ceIIs by fIow cytometry,and the expressions of Th17-specific transcription factor ROR-γt and Th17-specific marker IL-17A were measured by qRT-PCR and ELISA methods. Results:AII fifteen progeny mice were identified as LFA-1-/- genotype. Purity of CD4+CD62L+ na?ve T ceIIs separated by MACS was above 95%. Th17 ceIIs couId be induced by Iow-dose TGF-βcombined with IL-6,and the differentiation ratio was increased obviousIy when IL-23 was added. In inducing system containing TGF-β,IL-6 and IL-23,na?ve T ceIIs from LFA-1-/- mice produced more Th17 ceIIs than those from WT mice(17. 2% ± 1. 4% vs. 5. 7% ± 0. 2%,P<0. 001),expressions of ROR-γt mRNA and IL-17A mRNA were up-reguIated(P<0. 001),and IL-17A concentration in ceII cuIture supernatant was increased(P<0. 01). Conclusions:Deficiency of LFA-1 promotes the differentiation of mice na?ve T ceIIs into Th17 ceIIs in vitro.

According to the development of electronic devices and embedded system,a Holter system with high sampling efficiency and good performance is introduced in this paper.Besides,a compact type of ECG device is developed which can precisely record normal ECG,pacing ECG and dynamic ECG.This paper puts emphasis on such key aspects as hardware design with low power consumption,high-efficiency and real-time algorithm,magnitude storage devices and reliable playback interface in high speed.

Objective To study the quality standard of Gegenbao Soft Capsule.Methods The qualitative identification of puerarin and daidzein was carried out by TLC,and the contents of puerarin and daidzein were determined by HPLC.Results Puerarin and daidzein could be identified by TLC with high specificity.The linearity was from 0.214 to 1.42 ? g for puerarin and from 0.033 to 0.221 ? g for daidzein by HPLC,and the average recovery was 101.5 % for puerarin and 96.6 % for daidzein with RSD 1.6 % and 1.8 % respectively(n=6).Conclusion This method is simple,accurate and reliable for the quality control of Gegenbao Soft Capsule.

The aging trend in China′s population sets an creasing demand for attention on senile people from both legal and emotional senses,including detailed and thoughtful humanistic hospice care.The traditional clinical service concept which values living while neglects dying should be modified,with a novel and overall humanistic hospice care for the dying and family members,in order to achieve the social harmony under the novel life value which enjoys living and a peaceful dying.

[ABSTRACT]OBJECTIVEThe influnence of observation on the antisense cytokeratin 13 (CK13) gene in nasopharyngeal carcinoma HNE1 cell transplantation tumor radiation sensitivity.METHODSHNE1 cell lines can be divided into control group: the control group (HNE1 cell) and lentivirus (transfection slow virus empty carrier) group and experimental group: HNE1-anti-CK13a (transfection antisense CK13a slow virus) and HNE1-anti-CK13b (transfection antisense slow virus CK13b) four groups, set up a corresponding animal model, after radiotherapy by flow cytometry, immunohistochemistry, PCR, Tunel method and Westernblotting detection.RESULTSThe cell cyclede tection of plasmid transfection slow virus group compared with control group after radiotherapy G2/M phase of the block were significantly prolonged; Immunohistochemical results showed emigration tumor CK13 expression decreased in the experimental group; Tunel method to detect apoptosis necrosis rate found that the experimental group,apoptosis rate significantly decreased; Western blotting detection caspase3 apoptosis markers. PCR to detect CDC25mRNA level decreased obviously.CONCLUSIONAntisense CK13 gene by regulating the cell cycle and apoptosis can reduce HNE1 transplantation tumor radiotherapy of nasopharyngeal carcinoma (NPC) sensitivity, and with the caspase 3 apoptotic pathways and CDC25 signaling pathways.

The article expounds the relationship of medical ethics and medical humanities education in a doctrinal aspect.Medical ethics possesses a more essential and inherent nature compared with medical humanities education,thus serves as the fundamental motivation and guide of medical practice for medical students and staff.Their relationship is clearly understood and the curriculum structure of medical education reasonably adjusted.The following will be beneficial reference for medical ethics cultivation for medical students: to strengthen all sidedness,positivism and interest of medical ethics education,medical humanities education served as evidence and complement for medical ethics education;to demonstrate ethical characteristics,profundity and intention of medical humanities education,medical ethics education also served as the aim and gist of medical humanities education.