Objective To investigate androgen receptor (AR) expression in triple negative breast cancer(TNBC) patients and its predictive value for clinical outcomes.Methods Five hundred and sixty-eight primary breast cancer patients who received surgery treatment from January 2008 to January 2010 were included in this study.Tissue sections of surgical resection specimens were underwent estrogen receptor (ER),progesterone receptor (PR),and human epidermal growth factor receptor 2 (HER2) immunohisto chemical staining,according to the judgment for triple negative breast cancer and non triple negative breast cancer.Data on age,family history,menstruation,tumor size,tumor stage,tumor grade,pathological type and lymph node metastasis were collected for analysis.Immunohistochemical analysis of AR was done in tissue sections of triple negative breast cancer patients,Kaplan-Meier method was used to compare survival between AR(+) and AR(-) groups,and Log-rank method was used to determine the association between AR-positive expression and 5-year overall survival as well as disease free survival.Results Among 568 breast cancer patients,174 (30.6％)TNBC patients were identified,including 84 patiens with AR (-) expression and 90 patients with AR (+) expression.During 5 years' follow-up,reccurrence rate was 35.7％ (30/84) in AR (-) group and 20.0％ (18/90) in AR(+) group;5-year overall survival rate was 73.8％ in AR(-) group and 81.1％ in AR(+) group.There was no significant association between AR(+) expression and 5-year death or recurrence rate,while after adjusting for age,menstruation,tumor size and lymph node metastasis,AR (+) expression was significantly associated with decreased 5-year death and recurrence rate,adjusted hazard ratio (HR) was 0.794,95CI (0.430-1.021) and 0.722,95CI (0.451-0.965) respectively.Conclusion AR (-) expression is associated with increased risk of death and recurrence and is a promising predictive marker for poor clinical outcomes.

Macrophage activation syndrome (MAS)is a serious potentially life -threatening complication seen primarily in patients with systemic onset juvenile idiopathic arthritis (sJIA).Clinical symptoms include persis-tent fever,liver and spleen lymph node enlargement,cytopenia,hyperferritinemia,hypofibrinogenemia,hypertriglyceri-demia,coagulation disorders,and hemophagocytosis can be seen in the bone marrow.This article summarizes the characteristics of MAS occurring in the context of sJIA and discuss the recent advances in classification systems and management.

Her-2 targeting therapy is an important part of the treatment for breast cancer with Her -2 overexpression.However,the therapeutic effectiveness is largely influenced by Her -2 resistance.Previous studies have confirmed that PI3K/AKT/mTOR signaling pathway is activated during the process of drug resistance to Her-2-targeting therapy.Therefore,investigating the PI3K/AKT/mTOR signaling pathway and drugs -targeted pathway are important for the treatment of breast cancer .

Objective:To clone and express mouse fibroblast growth factor eight(FGF 8) and investigate the function of it Methods:According to the published sequence of mouse fibroblast growth factor 8, a pair of special primers was designed Then the full length cDNA of FGF 8b (a predominant isoform of FGF 8) was isolated from the total RNA of the mouse embryo by means of reverse transcription PCR and subcloned into the yeast expression vector of pYEX4T 1 in correct direction It was identified with sequencing The recombinant plasmid was transformed into yeasts The fusion protein expressed was verified by using Western blot The activity of the protein was examined by MTT and 3H TdR incorporation Results:The cDNA fragment was about 600 bps, and proved to be "b" isoform of FGF 8 exactly This fusion protein, with molecular weight about 55 kD, was highly expressed in yeast system The data of MTT and 3H TdR incorporation in NIH3T3 cells were increased obviously by the protein, and decreased by the antagonist Conclusion:Fibroblast growth factor eight was cloned and expressed.The protein was found to be capable of stimulating the proliferation of NIH3T3 cells Moreover, the recombinant protein of the extracellular fragment of FGFR1 can antagonize the response of NIH3T3 to the recombinant protein

Objective To explore the relationship between the subjective well-being and personality characteristics of nurses,and provide the basis for managers to implement humanized management and improve the nurse happiness from the perspective of positive psychology.Methods A total of 190 nurses were selected from a hospital of Zhongshan city by convenience sampling technique.And General Situation Questionnaire,Happiness Scale and the revised Eysenck Personality Questionnaire-Short Form (Chinese version) were used in the investigation.Results The average score of general happiness was (54.91± 9.18) points for male nurses,which was lower than that of the national norm.The sense of happiness between male and female nurses showed statistical difference.The emotional stability of nurses with different marital status showed significant difference.Nurses' subjective well-being and personality characteristics had a significant causal relationship by multiple stepwise regression analysis.Conclusions The general wellbeing about nursing staff is lower than the national norm,male nurses' happiness was lower than that of female and married nurses' emotional instability is higher than unmarried nurses.The nurses' personality is an important factor that affect their happiness.Managers should be under the guidance of the positive psychology,pay attention to the nurse' personality characteristics to improve the nurse' happiness,improve the level of nurse' mental health,to stabilize and optimize the nursing team,and improve the quality of nursing.

Objective To compare the influence of resin core on overall bending strength of fiber post-core restoration.Methods 60 mandibular first premolar extracted because of orthodontic treatment was selected.3M light -cured composite resin P60,medental dual-cured resin,pulpdent dual-cured resin combined with viva carbon fiber reinforced glass fiber post were applied.The changes of teeth in vitro were observed when strength was given on.Results The flexural strength of 3M light-cured composite resin was (80.182 ±9.512)N,Medental dual-cured resin was (87.805 ± 11.649) N,Pulpdent dual-cured resin was (85.458 ± 10.845) N.The flexural strength of 3 M light-cured composite resin was lower than that of medental dual-cured resin and pulpdent dual-cured resin (t =5.758,3.084,both P ＜ 0.05).There was no statistical differences of flexural strength between medental dual-cured resin and pulpdent dual-cured resin(t =0.718,P ＞ 0.05).There was large area of resin broken off after 3M light-cured composite resin core fracture.There was only fracture of medental dual-cured resin core and pulpdent dual-cured resin core.Conclusion The bending strength of dual-cured resin is better than that of highly filled light curing composite resin in large tooth hard tissue defect restoration with fiber post-core,and core broken off is rare.Dual-cured resin is better post-core materials.

[Summary] Chronic suppurative otitis media affects patients ’ quality of life, and is life-threatening in patients with cholesteatoma which causes intracranial or extracranial complications .The purpose of surgery is to clear up all the lesions and to reconstruct hearing function .Tympanoplasty is the main surgical method , which includes basically canal wall down ( CWD) and canal wall up (CWU) styles.CWD provides lager surgical field but impairs normal auditory canal structure ; CWU preserves the posterior wall of external auditory canal and provides better quality of life although it has higher possibility of recurrence of cholesteatoma .We reviewed the surgical methods , surgical effects , recurrence rates , and surgical indications of canal wall up mastoid-tympanoplasty , hoping to provide meaningful reference for clinical application .

SUMMARY Salivary gland tumors are a group of the most common tumors in the oral and maxillofacial region with obvious characteristics of clinical , histopathologic , and therapeutic aspects .During the past 50 years, our research group performed a systemic study on salivary gland tumors including imaging diag-nosis, fine needle aspiration cytology , FT-mid-IR fiber optics spectroscopy , histopathologic features and biological behaviour of the tumors , functional parotid surgery , and 125 I seed implant branchytherapy . More reasonable principles of management for various types of the tumors were put forward based on the histopathologic features of the tumors .The surgical techniques were modified and the postoperative com-plications were reduced obviously .Thus, the quality of life of the patients was improved greatly .

By summarizing and analyzing recent 10 years’ relevant literature, this article reviews advances in clinical studies of acupuncture treatment for oculomotor paralysis from acupuncture point selection, needling methods and scalp acupuncture and shows that acupuncture therapy has the characteristics and advantages of being simple, convenient, efficacious and cheap.

Objective To investigate the 9 . 4 T of DTT in the diagnosis of SD rat C6 cerebral gliomas model about the change of CST on tumor progression and pathology confirmed. Methods ①10 μl C6 cell suspension of 106 cells number were implanted into the right caudate nucleus of brain hemisphere of 20 adult male SD rat with ster-eotactic technique. ②Application of 9. 4 T of DTT and DTI, meanwhile the routine T1-weighted imaging(T1WI), T2-weighted imaging(T2WI), contrast-enhanced T1WI,FLAIR imaging and diffusion tensor of the brain were ac-quired. Anatomic relationship between cerebral glioma and surrounding white matter fiber tracts was analyzed and measured the values of FA and MD in different areas on fractional anisotropic ( FA) map, color-coded directional map, three-dimensional (3D) white matter fiber tracts map, then stained with routine HE. Results All of SD rat C6 cerebral gliomas models 3 D white matter fiber tracts map by means of FA maps of DTI were successfully comple-ted. Apparently significant differences of FA values were found in solid tumor, surrounding edema, compared with normal white matter regions ( P<0.05 );but there was no significant difference among solid tumor and surrounding edema region (P>0.05);there were significant differences of MD values between solid tumor, surrounding edema and normal white matter region ( P<0.05 ) . Conclusion The 9 . 4 T of DTT offers the optimal visualization of An-atomic relationship between cerebral glioma and surrounding white matter fiber tracts in the different periods of the tumor moder.