Acupuncture Therapy ; Adult ; Diabetes Complications ; microbiology ; therapy ; Female ; Humans ; Middle Aged ; Urinary Tract Infections ; microbiology ; therapy

Abscess ; surgery ; Foreign Bodies ; surgery ; Foreign-Body Migration ; Humans ; Thyroid Gland

Target restoration space (TRS) is the most precise space required for designing optimal prosthesis. TRS consists of an internal or external tooth space to confirm the esthetics and function of the final restoration. Therefore, assisted with quantitive analysis transfer, TRS quantitative analysis is a significant improvement for minimum tooth preparation. This article presents TRS quantity-related measurement, analysis, transfer, and internal relevance of three TR. classifications. Results reveal the close bond between precision and minimally invasive treatment. This study can be used to improve the comprehension and execution of precise esthetic prosthodontics.
Esthetics ; Esthetics, Dental ; Humans ; Prosthodontics ; methods ; Tooth Preparation

Objective To investigate the effect of different doses of propofol on cytokines-tumor necosis factor-? (TNF-?), interleukin-6 ( IL-6 ), interlenkin-8 ( IL-8 ), during systemic inflammatory response syndrome (SIRS) .Methods Fifty-six male Wistar rats weighing 190-220g were anesthetized with intraperitoneal 2.5% pentobarbital 30mg.kg-1 . The animals were randomly divided into 4 groups of 14 animals each: group A received intraperitoneal saline 10ml.kg-1 followed by intravenous infusion of saline (2ml.h-1 ); group B received intraperitoneal LPS 1mg.kg followed by intravenous infusion of saline(2ml.h-1 ) ; in group C propofol was infused iv at 75?g.kg-1.min for 4h after intraperitoneal LPS injection; in group D propofol was infusion iv at 150?g.kg-1 .min-1 for 4h after intraperitoneal LPS injection. Blood samples were taken before experiment and 1, 2, 3, 4h after saline or propofol infusion for determination of serum TNF-? (cytotoxin stain method) and IL-6, IL-8 (ELISA) concentration.Results Serum concentrations of TNF-?, IL-6 and IL-8 induced by LPS increased significantly in group B as compared with those in group A. Propofol decreased the level of cytokines and delayed the peak values of TNF-? and IL-8. Conclusion Propofol used in the early stage of SIRS has some protective efficacy.

AIM: To establish cell line FL-POL? - and to study the role of POL?(polymerase kappa) on genetic stability. METHODS: A mammalian expression vector expressing antisense POL? gene fragment pMAMneo -amp -- POL? was constructed by cloning the 1 690-1 918 fragment of POL? gene into the mammalian expression vector pMAMneo-amp - in antisense orientation. FL cells were fransfected with this antisense RNA expressing vector and selected by G418. Based on the shuttle-plasmid pZ189, the mutation assay was made. RESULTS: The spontaneous mutation frequency of supF tRNA gene in the plasmid replicated in the FL-POL? - was 11.2?10 -4 , while it was 4.9?10 -4 and 3.7?10 -4 in the control cells FL and FL-M , respectively. CONCLUSION: POL? playes an important role in maintenance of genetic stability.

Da Vinci robotic surgical system have some advantages of defibrillation,three-dimensional (3D) view and flexible operation,and it has been gradually applied in radical gastrectomy,colorectal cancer radical resection,complex hepatic resection,biliary tract surgery,partial resection of pancreas and pancreaticoduodenectomy in the fiell of digestive surgery,with satisfactory clinical efficacy.Compared with traditional laparoscopic and open operations,Da Vinci robotic surgical system is more precise and flexible,with advantages of minimal invasion and good application value,however,longer operation time,expensive surgery cost and indefinite long-term efficacy of malignant tumor need to be further improved.

Calcium-sensing receptor(CaSR) is a widely distributed G-protein coupled receptor.The activated CaSR plays an important role in many kinds of signaling pathway regulation, such as Ca2+ signaling pathway.It not only maintains the body calcium balance, but also is involved in the regulation of a variety of cell secretion, proliferation, apoptosis, and ion channel opening processes.CaSR expression is involved in stem cell migration, adhesion and homing in hematopoietic stem cells and mesenchymal stem cells.The activated CaSR also regulated the function of itself and characteristics in stem cells through a variety of cell signaling pathways.We introduce the functions and characteristics of CaSR, the relationship between CaSR and disease, and review the effects of the biological characteristics on hematopoietic stem cells and mesenchymal stem cells.

Objective To study the result of DSA for 56 cases with large liver cancer that there is bloody supply from arteria musculophrenica right and the clinical value of super selective interventional embolization,so that to improve the clinical curative effects of interventional therapy. Methods Employ 4F, 3F catheter super selectively into arteria musculophrenica that supply blood to cancer. Result 56 cases(21.7% )were discoverod that there was bloody supply from arteria musculphrenica right to cancer in 258 cases with large liver cancer, liver neoplastic size of all cases wasin 5～15cm,22 cases(8.6% ) were discovered in the first interventional operation,34 cases ( 13.3% )were discovered in the second or the third interventional operation. Obvious tumor shrinking in all cases: the 6month survival as 56 cases(100 % ), the 1-year survival as 45 cases (80.4%), the 2-year survival as 21 cases(37.5%). Conchssion Arteria musculophrenica right supply blood to cancer is common in large liver cancer eases, super selectively embolized the supply blood from the arteria musculophrenica right is very important for acquiring success by interventional operation.

Objective To identity bacteria of bacteroides group by PCR.Method The universal primers were designed according to the sequence of 16S rRNA,then 13 strains bacteroides and the other non-bacteroides were amplified by PCR. PCR products were then sequenced and analyzed by Blast.Results Thirteen strains of bacteroides were amplified, and the non-bacteroides were not amplified. The pairs of primers had highly specificity.Comparing the gene sequences of the 13 strains,bacteroides with corresponding standard 16S rRNA sequences,the homology was from 81% to 100%.Conclusion The established PCR assay could specially amplify bacteroides and had good sensitivity with 100 genomes/ml.

Objective: To investigate the effect of sinomenine on expression of toll like receptor(TLR) 2 and TLR4 of dendritic cells in patients with rheumatoid arthritis in vitro.Methods: The peripheral blood mononuclear cells were isolated from 10 rheumatoid arthritis patients and differentiated into dendritic cells by stimulating with cytokines.Dendritic cells were exposed to high(5mM),middle(2mM),low(1mM) concentration sinomenine and control medium.TLR2 and TLR4 mRNA were detected by real time PCR.Western blot was used to measure the expression of TLR2 and TLR4.Results: In contrast to control,signifi cant lower expression of TLR2,TLR4 mRNA and protein were found in dendritic cells treated with sinomenine,especially in high and middle concentration.Conclusion: The therapeutical effect of sinomenine on rheumatoid arthritis is probably by inhibiting dendritic cells expressing TLR,which can transduce the in? ammatory signal.