Objectives:To investigate the prevalence situation and antibiotic resistance of chlamydia trachomatis (CT),ureaplasma urealyticum(UU) and mycoplasma hominis(MH) in infertile female patient,and to analyze the resistant of mycoplasma to common antibiotics. Methods:The cervical secretion samples from 2186 female infertility and 210 pregnancies women were collected. Then chlamydia trachomatis antigen was detected by immunoassay. Mycoplasma (UU and MH) were isolated and tested antimicrobial susceptibility for 12 kinds of antimicrobial drugs. Results:In the trial group 173 were CT-positive at a rate of 7.9%,and 1102 were positive for the mycoplasmae at a rate of 50.4%,of which 987 were UU infections and 115 were MH infections.The number of CT,UU and MH infections totaled 1275 cases,leading to an overall infection rate of 58.3%. The top three antibiotics for drug sensitivity in the UU,MH and UU+MH cultures were josamycin,minocycline and clarithromycin.The three antibiotics,to which the pathogens were most tolerant,included ofloxacin,norofloxacin and sparfloxacin. Conclusion:The infection rate of chlamydia and mycoplasma in infertility women is obviously higher than normal pregnanted,this shows the fact that chlamydia and mycoplasma infection of genitourinary tract may be one reason of infertility.The sensitivity of mycoplasma to common antibiotics especially to quinolones and macrolides has decreased.

Chronic lung disease is a very common complication caused by inhaled hyperoxia, mechanical ventilation and pulmonary infection in preterm infants. It shows early inflammation and late alveoli fusion with mesenchymal fibroblast proliferation. Mitogen activated protein kinase is a very important signal transduction pathway in eukaryotic cells.It plays an important role in the cell inflammation, proliferation, differentiation, survival and apoptosis, which may contributes to the chronic lung disease.

Dentin Sensitivity ; prevention & control ; Humans

Dental Care for Aged ; Dentures ; Humans ; Oral Health ; Oral Hygiene ; Practice Guidelines as Topic

Objective:To investigate the effect of microRNA-144-3p (miRNA-144-3p) on drug resistance sensitivity of thyroid cancer cells by targeting and regulating paired box gene 8 (PAX8).Methods:Human thyroid cancer cell line FTC-133 was cultured in vitro and selected to construct the cisplatin-resistant cell stains. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the relative expression of miRNA-144-3p in FTC-133 cells and cisplatin-resistant FTC-133 cells. The cisplatin-resistant FTC-133 cells were divided into group A, group B, and group C. Group A received no treatment, group B was transfected with empty plasmids, and group C was transfected with pCDNA3.1+ miRNA-144-3p plasmids. RT-PCR was used to detect the relative expression levels of miRNA-144-3p and PAX8 in each group, and methyl thiazolyl tetrazolium (MTT) method was used to detect the half inhibitory concentration (IC 50) value of cisplatin on cells in each group, proliferation rate in each group was detected by cell counting kit-8 (CCK-8) method, and apoptosis rate in each group was detected by flow cytometry. Results:The relative expression level of miRNA-144-3p in cisplatin-resistant FTC-133 cells was significantly higher than that of FTC-133 cells [(0.93±0.24) vs (0.26±0.04), P<0.05]; The IC 50 value, proliferation rate, apoptosis rate and relative expression levels of miRNA-144-3p and PAX8 in cisplatin-resistant FTC-133 cells in group B were not significantly different from those in group A ( P>0.05). The IC 50 value, proliferation rate and relative expression of miRNA-144-3p of cisplatin resistant FTC-133 cells in group C were significantly higher than those in group B ( P<0.05), and apoptosis rate and relative expression of PAX8 were significantly lower than those in group B ( P<0.05). Conclusions:Overexpression of miRNA-144-3p may increase cisplatin resistance of thyroid cancer cells by up-regulating PAX8 gene expression, thus promoting the proliferation of thyroid cancer cells and inhibiting their apoptosis.

Objective:To investigate the effects of radix actinidiae chinensis extracts on the proliferation and apoptosis of breast cancer cells by regulating the vascular endothelial growth factor (VEGF)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.Methods:Human breast cancer cell line MCF7 was cultured in vitro and divided into control group, low-dose group, medium dose group and high-dose group. The low-dose, medium dose and high-dose groups were added with DMEM culture medium diluted with 1, 10 and 100 μg/ml radix actinidiae chinensis extracts respectively. The control group was added with equal dose DMEM culture medium for 48 hours. The proliferation rate (detected by methyl thiazolyl tetrazolium method), apoptosis rate (detected by flow cytometry) and the protein expression of PI3K, VEGF and phosphorylation-AKT(p-AKT) in each group were compared (detected by Western blot). Results:Compared with the control group, the proliferation rate and PI3K, VEGF, p-AKT protein expression of MCF7 cells in low dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in low dose group was significantly increased ( P<0.05); compared with low dose group, the proliferation rate and PI3K, VEGF, p-AKT protein expression of MCF7 cells in medium dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in medium dose group was significantly increased ( P<0.05). Compared with the middle dose group, the proliferation rate of MCF7 cells and the expression of PI3K, VEGF and p-AKT protein in the high dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in the high dose group was significantly increased ( P<0.05). Conclusions:Radix actinidiae chinensis extracts may inhibit the proliferation and promote the apoptosis of breast cancer cells by inhibiting the VEGF/PI3K/AKT signaling pathway.

Objective: To establish a method of methylation-sensitive restriction enzymes-quantitative PCR (MSRE-qPCR) for methylation analysis of adenomatous polyposis coli (APC ) gene, and to further assess the clinical value of plasma methylation detection by using this method in diagnosis of hepatocellular carcinoma (HCC). Methods: Hha I was used to digest genomic DNA, and the digestion efficiency was evaluated by using qPCR technique. Then the optimized MSRE-qPCR method was established. The methylation levels of APC in 45 liver tissues (20 surgically resected HCC specimens and the matched non-cancerous tissues, as well as 5 normal liver tissues) were detected by MSRE-qPCR, and then further validated by using bisulfite sequencing PCR (BSP). The results of MSRE-qPCR were compared with those of methylation-specific PCR (MSP) assay. MSRE-qPCR method was used to detect the APC methylation status of plasma samples from 72 cases of H CC, 37 cases of benign liver diseases and 41 healthy volunteers. Results: The established MSRE-qPCR method could detect as low as 1% methylated target sites in given DNA samples. The results of MSRE-qPCR and MSP showed that APC gene was hypermethylated in HCC tissues. The result of MSRE-qPCR was verified by BSP, and it was comparable with that of MSP (Kappa=0.955, P<.000 1). Methylation level of plasma APC in patients with H CC was significant higher than those in patients with benign liver diseases and the healthy volunteers (P<.000 1).Combined analysis of plasma APC methylation and serum alpha-fetoprotein (AFP) revealed an increased diagnostic efficacy for HCC. Conclusion: MSRE-qPCR is a method for quantitative analysis of APC methylation level. Methylation analysis of plasma APC is a valuable method for the noninvasive diagnosis of HCC. Copyright© 2011 by TUMOR.

Objective To investigate the effect of anti-snoring pillow for the treatment of obstructive sleep apnea in patients with heart failure.Methods Forty seven HF patients with OSAHS and 12 HF patients with simple snoring were enrolled.All the patients were divided into 3 groups:control group (n =12),mild-moderate OSAHS group (n =26) and severe OSAHS group (n =21).All the patients received the treatment of anti-snoring pillow and their sleep apnea parameters were evaluated by PSG at the same time.Results The sleep apnea parameters including AHI,OAI,ODI,mean SpO2％ and snore index were significantly improved in severe and mild moderate OSAHS group with anti-snoring pillow.Conclusion Anti-snoring pillow can reduce snoring index in all OSAHS patients and simple snorers.Sleep apnea and hypoxemia were greatly improved in mild-moderate OSAHS-HF patient while the anti-snoring pillow is not perfect in severe OSAHS patients with HF to replace PAP.

The International Society on Thrombosis and Haemostasis has proposed to hold World Thrombosis Day on October 13 annually from 2014 to improve awareness and education of thrombosis. Thrombotic diseases are multidisciplinary disorders involving multiple systems.Thus, multidisciplinary team treatment for thrombotic diseases should be developed in China.A comprehensive study of genetic factors for thrombotic diseases is expected to achieve early diagnosis and risk prediction for this type of disease, and thus precision diagnosis and individualized treatment can be achieved.

Severe burn is often accompanied by multiple organ damage. Acute lung injury (ALI) is one of the most common complications, and often occurs in the early stage of severe burns. If it is not treated in time, it will progress to acute respiratory distress syndrome (ARDS), which will be a serious threat to the lives of patients. At present, the treatment of ALI in patients with severe burn is still remained in some common ways, such as the liquid resuscitation, the primary wound treatment, ventilation support, and anti-infection. In recently, human umbilical cord mesenchymal stem cells (hUCMSCs) have been found having some good effects on ALI caused by various causes, but few reports on the efficacy of ALI caused by severe burns were reported. By reviewing the mechanism of stem cell therapy for ALI, therapeutic potential of hUCMSCs in the treatment of severe burns with ALI and a new approach for clinical treatment was provided.