ObjectiveTo explore the clinical effect and safety evaluation of marvelon on dysfunctional uterine bleeding in adolescent patients.Methods120 adolescent patients with dysfunctional uterine bleeding were randomly divided into control group and experience group with 60 patients in each group.Control group was given oral administration of premarin and experience group received marvelon oral treatment.Clinical effect,the time of control bleeding and completely stop bleeding and incidence of adverse reaction were compared between two groups.ResultsThe total effective rate in experience group was much higher than that in control group (100.0％ vs 90.0％,x2 =6.316,P ＜ 0.01 ) ;The time of control bleeding and completely stop bleeding in experience group were much lower than that in control group( t =6.638,8.542,all P ＜ 0.01 );The incidence of adverse reaction in experience group and control group were 11.7％ and 65.0％ respectively with significant difference( x2 =36.099,all P ＜0.01 ).ConclusionMarvelon on dysfunctional uterine bleeding in adolescent patients is beneficial to rapid and effective hemostasis with less adverse reaction rate and better tolerance.

Objective To investigate the cost-effectiveness of four drugs in treatment of bacterial vaginitis and improve clinic rational drug use.Methods 361 bacterial vaginitis out-patients were treated with lactobacillus vaginal effervescent tablets(92 cases),lacidophilin vaginal capsules(88 cases),shuangzuotai vaginal effervescent tablets(90 cases),metronidazole vaginal effervescent tablets(91 cases).The therapeutic effect,drug cost and therapeutic effect were evaluated by cost effectiveness analysis 7d after treatment.Results The overall response rate of lactobacillus vaginal effervescent tablets,lacidophilin vaginal capsules,shuangzuotai vaginal effervescent tablets,metronidazole vaginal effervescent tablets were 78.26％,76.14％,83.33％ and 78.02％,respectively.The overall response rate was no significant difference between four groups (0.06),and metronidazole vaginal effervescent tablets had the lowest cost effectiveness rate (C/E,0.26).Conclusion The metronidazole vaginal effervescent tablets is better schedule in treatment of bacterial vaginitis.

Development of primary angle closure glaucoma (PACG) is closely related to the changes of the position and morphology of the lens owing to a shallow anterior chamber,pupillary blockage and angle closure.Lens extraction can deepen the depth of the anterior chamber and resolve the pupillary blockage,and therefore increase the outflow of aqueous fluid.Furthermore,the combination of lens extraction with goniosynechialysis can reopen the closed anterior chamber angle and relieve peripheral anterior synechia of pupil,with a better clinical effectiveness for primary angle closure glaucoma.Recently,the studies related to lens extraction for primary angle closure glaucoma have madc great progression,especially its mechanism and efficacy,and of course some existing problems of lens extraction are concerned.In this review,the relationship between lens and primary angle closure glaucoma,the clinical effectiveness of lens extraction for primary angle closure glaucoma,the safety evaluation of lens extraction and goniosynechialysis were summarized.

Background Idiopathic macular epiretinal membrane (IMEM) shows a high incidence and affects visual function of patients.The primary management of IMEM is vitrectomy,but the study on prediction of visual acuity after operation by optical coherence tomography (OCT) is seldom.Objective This study was to evaluate the predicting ability of OCT for preoperative visual outcome for IMEM patients who received vitrectomy.Methods The clinical data of IMEM patients with vitrectomy from March 2009 to May 2013 were retrospectively analyzed in People's Hospital Peking University.Forty-nine eyes of 48 cases were divided into continuous group (19 eyes of 18 cases) and discontinuous group (31 eyes of 31 cases) based on whether the extraretinal layers were intact on OCT,including retinal pigment epithelium (RPE),inner and outer segment of photoreceptors (IS/OS) and external limiting membrane (ELM).Central foveal thickness (CFT) by high-resolution spectral-domain OCT and best corrected visual acuity (BCVA) (LogMAR) before surgery and 12 weeks after surgery were recorded and compared between the two groups,and the correlations of shift of CFT with the shift of LogMAR vision was analyzed by unitary linear regression to evaluate the value of OCT in predicting postoperative vision.Writen informed consent was obtained from each patient prior to any medical operation.Written informed consent was obtained from each patient before surgery.Results In 12 weeks after operation,reflective strips of RPE layer,IS/OS and ELM layer were intact in 18 eyes of the continuous group after vitrectomy.The number of eyes with discontinuous strip in RPE layer decreased to 1 eye after operation from 6 eyes before operation,and the number of eyes with IS/OS reflective strip discontinue decreased to 19 eyes after operation from 29 eyes before operation,and that with ELM layer discontinue decreased to 15 eyes after operation from before operation in the discontinuous group.The improvement of LogMAR BCVA was increased with the decline of CFT values with a negative correlation between them (R2 =0.298,B =0.001,P =0.000),and postoperative LogMAR visual acuity improved 1 line whenever CFT reduced by 100 μm.LogMAR BCVA was 0.4±0.19 before surgery and 0.36±0.21after surgery in the continuous group,showing a insignificant difference between them (t =0.876,P=0.393).In the discontinuous group,the postoperative IogMAR BCVA was significantly higher after operation (0.46±0.26) than 0.82 ±0.41 after operation (t =6.206,P =0.000).The increase value of LogMAR after vitrectomy was 0.3 in the discontinuous group,which was superior to 0.0 of the continuous group.Conclusions The continuity of extraretinal layers and CFT by OCT are closely associated with the improvement of vision after vitrectomy in the eyes with IMEM.Postoperative vision is better in IMEM eye with continuous retinal reflective strips before operation,and majority of IMEM eyes can improve vision after operation.Preoperative OCT seems to be an important diagnostic tool for the selection of patients who benefit or not from surgery to certain extent.

The data of pediatric patients who required sedation for non-invasive examinations, aged 1 day-13 yr, were retrospectively collected from December 2019 to June 2020.They are divided into 4 groups according to age: newborn (age 0-28 days) group, infant (age 29 day-1 yr) group, toddler (age 1-3 yr) group, and child (age 4-13 yr) group.Chloral hydrate 50 mg/kg was administrated orally.When the Ramsay score was ≤ 4 points at 30 min after administration, oral chloral hydrate 25 mg/kg or intranasal midazolam 0.1 mg/kg or intranasal dexmedetomidine 1 μg/kg was given as rescue administration.If the Ramsay score was still ≤ 4 points at 30 min after rescue administration, the examination was rescheduled or rescue drugs were given again by the anesthesiologists according to the condition and the urgency of the examination, with the number of administration not exceeding 3 times.The sedation failure was defined that the patients could not complete the diagnostic procedures after administration due to various reasons (including severe respiratory depression, rescheduled examination, parents' refusal to rescue administration, and Ramsay score still ≤ 4 points after 3 times of administration). The success of sedation at first attempt, success of rescue sedation and success of total sedation were recorded.The occurrence of sedation-related adverse events such as coughing, vomiting, allergies and respiratory depression was recorded.Compared with neonate group, the success rate of sedation at first attempt and success rate of total sedation were significantly increased, the incidence of sedation-related adverse events was decreased in infant, toddler and child groups ( P<0.01). There was no significant difference in the parameters mentioned above among infant group, toddler group and child group ( P>0.05). In conclusion, the efficacy of chloral hydrate for sedation is not good when used in neonates undergoing non-invasive diagnostic procedures.

Objective To study the effect and the mechanism of baicalin on invasion and metastasis of(human) hepatocellular line BEL-7402.Methods Human hepatocellular line BEL-7402 were cultured and treated respectively with 5,10,20 mg/L baicalin for 48 h.The invasion and adhesion of the BEL-7402 cells were examined in Boyden chamber,cell migration and morphology were also observed.The expression of MMP_(2),TIMP_(2),_()integrin ?_(1,)and E-cd proteins of human hepatocellular line BEL-7402 was determined by flow cytometry.Results Baicalin inhibited invasion,adhesion and migration of hepatocellular line BEL-7402 in vitro in a dose dependent manner(P

Objective: To investigate the effect of GLP1 on the blood glucose in type 2 diabetic rats,and its protective effects on the islet B cells.Methods: Thirty rats were divided into three groups: spontaneous type 2 diabetes animal model OLETF rats,GLP-1 [from the twelfth week 56 ?g/(kg?d),sc] therapy group and LETO rats as control.In the 14th and 20th weeks,standard OGTT including fast and 2 h-plasma glucose were measured respectively.In the 14th weeks,3 rats from each group were killed randomly,and the rest of the rats were killed until the 20th week.Immunostaining with the marker of PCNA,TUNEL,and insulin assessed metabolic changes in the islet. Ultrastructure of the B cell was observed with the electronic microscope.Results: In the 14th and 20th week,AUC for insulin were higher in treated animals(10.86?1.56 vs.9.07?1.28,P

Objective To explore the effect of docosahexaenoic acid(DHA)on the neuro-logical outcome as well as the expression of interleukin-1β,interleukin-6 and interleukin-10 after acute spinal cord injury(SCI).Methods One hundred and eight Sprague-Dawley rats were randomly divided into a spinal cord injury group(group A),a DHA treatment group(group B)and a sham operation group(group C),each with 36 rats.SCI models were established in the rats of groups A and B using a version of Allen's weight drop method(T10 level).Group C had removal of the lamina only.Ten minutes later,group A was injected with 5% ethanol in saline(the MT solvent)and group B with 500 mg/kg of DHA preparation.Basso beattie bresnahan locomotor rating scale(BBB)was used to detect locomotors function at 10,24,48 and 72 hours after operation.Rats were sacrificed at 72 h after operation,IL-1β,IL-6 and IL-10 levels in serum were detected in 6 rats of each group.Results BBB locomotor rating scale indicated that hindlimb motor function in Groups B was higher than that in Group A at 10,24,48 and 72 hours after operation.There was a significant difference in locomotor rating score between DHA injection group and control group(P<0.05).The specimens of group A showed inflammatory reaction and ulceration at 72 h:Group C showed the highest levels of IL-10 in serum ,while Group A showed the lowest level.The differences were statistically significant.Group A had the highest levels of IL-1β and IL-6 while group C had the lowest.The difference between group B and groups A and C was significant.Conclusion DHA could play protective role by balancing the reconstruction of the anti-inflammatory response and inflammation in rats with acute spinal cord injury.

Gastric cancer is a kind of disease with high incidence and mortality rate,most gastric cancer cases are diagnosed in an advanced,and chemotherapy is the main treatment.Drug resistance is one of the most important causes of therapy failure in gastric cancer patients.It has revealed that the mechanism of tumor resistance mainly involves alterations in cell cycle and proliferation,enhanced DNA repair capacity,defective apoptosis,damaged host immune,altered metabolism of drugs and so on.In recent years,an important molecule-microRNA(miRNA)that regulates the biological behavior of cell has attracted more and more attention.Studies have confirmed that numerous miRNAs can regulate the drug resistance of gastric cancer cells via BCL-2 signaling pathway,PTEN/PI3K/AKT pathway,ABCB1(MDR1/P-gp),autophagy,cell cycle and so on.

Analysis of metabolic activities of cytochrome P450 isoenzyme is a crucial index to study drug/toxicant metabolism, drug-drug interaction, polymorphism and et al. Due to this practice, it is important to use the proper probe substrate and to conduct the experiment under optimal conditions. The validation information in literatures on the most common and newest in vitro probe substrates have been reviewed.