Adult male rats weighing 250-300 g were used to study ACPase and ATPase in the liver following 60Co radiation on the whole body for a single dose 2000 rad(95.7-99r/min). The irradiated group and the control group were sacrificed at intervals of 1, 2, 3 days after irradiation. The left liver lobe was removed, fro/en in dryice-isopentane, and then cut in a cryostat. ACPase and ATPase activities were revealed by histochemical and cytochemical methods respectively.In the control group, ACPase activity was found to be mainly localized in the vicinity of bile canaliculi, and generally higher in the periportal region than in the centrilobular region. The distribution of ATPase reaction products was much like that of ACPase.On the 1st day after irradiation, a slight decrease was found in ACPase and ATPase activities, while on the 2nd and 3rd days in both of them was found a marked increase especially in the periportal region. These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.

Autoimmune diseases are characterized by the presence of various autoantibodies and may cause injuries to multiple organs,with kidney as the most common and important organ involved.Autoantibodies are of great importance in the diagnosis,treatment and prognosis of autoimmune renal diseases.Lupus nephritis,anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis and antiglomerular basement membrane (GBM) disease are the most common autoimmune renal diseases.Anti-C1q antibody,ANCA and anti-GBM antibody play important roles in those diseases,respectively.Appropriate and steady detecting methods are crucial to clinicians,and the results should also be interpreted with great cautions.

Application research on human amniotic membrane has been carried out for nearly a hundred years and people found that there were more than dozens of kinds bioactive substances in the amniotic membrane. It has been proved that the amniotic membrane has a lot of functions, such as anti-inflammatory, anti-bacterial, anti-virus, anti-angiogenic and promoting cell apoptosis, and soon. As effective treatments, amniotic membrane has been used for adjunctive therapy of burns, trauma, ophthalmic damage, dermatopathya. Recent advances of amniotic membrane and amniotic membrane-derived cells research have led to enormous progress in skin tissue engineering, vascular tis- sue engineering, biological scaffold material, and biological sustained-release materials. Amniotic membrane and amniotic membrane derived cells have a significant advantage and unique charm in medical field. Therefore, they have higher research value and broad prospects in the applications.
Amnion ; Biomedical Research ; trends ; Humans ; Tissue Engineering ; Treatment Outcome

Crush Syndrome ; therapy ; Disasters ; Earthquakes ; Humans

0.05).Both SBP and DBP were declined significantly in both two groups(P0.05).Heart rate decreased from (81?9)b/min to (68?7)b/min in bisorpolol group.But there wasn't this effect in contrast group.It was superior to that of contrast group significantly too(P

Objective To report the experience of first 100 cases of off-pump coronary artery bypass(OPCAB) grafting by a training cardiac surgeon,and to discuss the feasibility of the training method.Methods The trainee,who had no previous experience of coronary surgery,received an OPCAB training program under the guidance by a director who had completed more than 2000 cases OPCAB.After 28 months of training,the trainee began to be an independent operator.The data of the first consecutive 100 cases of OPCAB by the trainee(Training Group) were collected for comparison with the director's 100 cases of OPCAB surgery(Control Group).Results There were 5 cases of conversion to on-pump surgery in the Training Group.The number of distal anastmosis in the Training Group(2.9?0.6) was smaller than that in the Control Group(3.5?0.7)(t=-6.508,P=0.001).Compared with the Control Group,there were less circumflex grafts(67/100 vs 88/100;?~2=12.645,P=0.000) and sequential grafts(3/100 vs 27/100;?~2=22.588,P=0.000) in the Training Group.There was no significant difference in mortality between the Training Group(3%,3 cases) and the Control Group(1%,1 case)(?~2=0.255,P=0.614).No significant difference was observed between the two groups in other postoperative severe complications.Conclusions The first 100 cases of OPCAB surgery performed by the trainee give favorable results,indicating a stable transition from training period to independent performance.It also testifies the feasibility of this training program.

AIM: To investigate the effects of oxidized low density lipoprotein (OX-LDL) on expression of matrix metalloproteinase-2 (MMP-2) in human umbilical vein endothelial cells (HUVEC). METHODS: The MMP-2 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), MMP-2 protein was measured by western blotting and MMP-2 activity in conditioned medium was observed by gelatin zymography. RESULTS: In comparison with the control, there was no difference in the expression of MMP-2 between control and 25 mg/L LDL, whereas 25 mg/L OX-LDL increased MMP-2 activity in HUVEC ( P

Bone Resorption ; China ; Cytokines ; physiology ; Diphosphonates ; pharmacology ; Estrogens ; pharmacology ; Humans ; Jaw ; drug effects ; metabolism

Activin is a growth factor belong to the transforming growth factor-? superfamily.Recently,experimental studies have shown that activin and activin A expressed higher in various brain injury models and activin have beneficial roles to neuronal protection.The high correlation of activin and activin A with clinical and biochemical signs of brain injury lead us to suggest that activin and activin A are new possible indicators to predict and treat brain injury.

The connotation development of industry and education is the road to advance the development of connotation and the quality of higher vocational and technical education.Based on this and bringing into play the advantages of running a vocational school and combined with cultivating the applied personnel needed by enterprises and professions,the college perfects the personnel cultivation model.carries out the "225" education model,accelerate the development of the teacher,promote its service capacity to enhance constantly the quality of higher vocational and technical education.