Adult male rats weighing 250-300 g were used to study ACPase and ATPase in the liver following 60Co radiation on the whole body for a single dose 2000 rad(95.7-99r/min). The irradiated group and the control group were sacrificed at intervals of 1, 2, 3 days after irradiation. The left liver lobe was removed, fro/en in dryice-isopentane, and then cut in a cryostat. ACPase and ATPase activities were revealed by histochemical and cytochemical methods respectively.In the control group, ACPase activity was found to be mainly localized in the vicinity of bile canaliculi, and generally higher in the periportal region than in the centrilobular region. The distribution of ATPase reaction products was much like that of ACPase.On the 1st day after irradiation, a slight decrease was found in ACPase and ATPase activities, while on the 2nd and 3rd days in both of them was found a marked increase especially in the periportal region. These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.

Autoimmune diseases are characterized by the presence of various autoantibodies and may cause injuries to multiple organs,with kidney as the most common and important organ involved.Autoantibodies are of great importance in the diagnosis,treatment and prognosis of autoimmune renal diseases.Lupus nephritis,anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis and antiglomerular basement membrane (GBM) disease are the most common autoimmune renal diseases.Anti-C1q antibody,ANCA and anti-GBM antibody play important roles in those diseases,respectively.Appropriate and steady detecting methods are crucial to clinicians,and the results should also be interpreted with great cautions.

Objective To observe the protective effect of Danggui Buxue Decoction on 60 Co-γray irradiated mice. Methods The experimental mice were randomly divided into 5 groups ( the normal control group , model group , Danggui Buxue Decoction of low , middle and high dose groups ) .The mouse except the normal control group were irradiated by 60Co-γray and established the radiation-damage model.Peripheral blood neutrophil count (NEUT), the number of bone marrow nucleated cells ( BMC ) , the activity of serum superoxide dismutase ( SOD ) and the serum levels of malondialdehyde ( MDA) were measured .Results Radiation injury in mice of NEUT , BMC and SOD were significantly decreased (P <0.01), MDA were increased (P <0.01).Danggui Buxue Decoction can increase the levels of NEUT , BMC and SOD in mice after radiation injury , decrease the content of MDA .And protective effect of high dose group was better (P <0.01).Conclusions Danggui Buxue Decoction has protective effect on radiation injury in mice .And there is a correlation between the protection effect and dose .

BACKGROUND:Despite regular mesh hydroxyapatite has certain biocompatibility and bone conductivity, it cannot be absorbed by human body after transplantation, and wil affect the growth and eruption of maxila and fangs when using for filing the injured alveolar cleft. OBJECTIVE: To compare the effect of conventional and new mesh hydroxyapatite composites in repair of alveolar cleft defects. METHODS: Totaly 46 patients with cleft lip and palate were randomly and evenly divided into test and control groups, and al were arranged according to cleft lip and palate sequence treatment. After nasal soft tissue defect repair, new mesh hydroxyapatite composite bone material (hydroxyapatite and bone morphogenetic protein compound). Conventional mesh hydroxyapatite material was adopted in the test and control groups, respectively. Within 6 months after the repair, the vertical distance from the newly formed bone tissue plane to the top of alveolar ridge was observed. The clinical repair effect was comprehensively evaluated from the aspects of complications, alveolar cleft shape and size, calus formation and material absorption. RESULTS AND CONCLUSION: At 2, 4, 8, 12 and 24 weeks, the vertical distance from the newly formed bone tissue plane to the top of alveolar ridge in the test group was higher than that in the control group (P < 0.05). The clinical repairing significant efficiency and total effective rate were al higher than those in the control group (P< 0.05). These results show that new mesh hydroxyapatite composite bone materials in the repair of alveolar cleft defects have good bone osteoinductivity and biocompatibility, rare complications and rapid calus formation.

Inherited epidermolysis bullosa (EB) is a group of hereditary skin diseases characterized by increases in skin brittleness and being prone to skin blisters or erosions when the skin is exposed to slight friction or injury. At present, it is classified into four types, namely EB simplex, junctional EB, dystrophic EB and Kindler syndrome. An onion skinning approach is recommended for the diagnosis of inherited epedermolysis bullosa, in which, immunofluorescence mapping is performed firstly, and causative genetic loci are then determined through detection of relevant genes. In its treatment, skin care should be intensified, and medical or surgical treatment may be used to alleviate symptoms. Further development of cell?, protein?and gene?based therapies is expected to bring hope to patients via realization of timely prenatal diagnosis and avoidance of adverse consequences.

As one of active immune effector molecules,immunoglobulins play an important role in the pathogenesis,diagnosis and treatment of autoimmune bullous diseases.Human immunoglobulins can be divided into 5 categories,including IgG,IgA,IgM,IgD and IgE,and all of them participate in and play different roles in the pathogenesis of autoimmune bullous diseases except IgD.IgG can induce the formation of blisters by activating complements and leukocytes as well as by releasing proteolytic enzymes,and different subtypes of IgG differ in biological activities.IgA can cause granulocyte migration and lead to the formation of blisters and pustules.IgE is associated with urticaria-like erythema and eosinophil infiltration.IgM is usually seen in Brazilian pemphigus foliaceus.These findings may provide new ideas for the diagnosis and treatment of autoimmune bullous diseases.

Objective To improve quality of wearable nurse call system producing the best possible results,and to provide safe,high quality nursing service.Methods The improvement plan was designed through team building,baseline assessment and defect analysis.Effective measures were formulated and implemented,and the outcomes were followed up.Results Average call response time was reduced from 49.23±5.71 s to 16.62±3.83s.Patient satisfaction of response time was increased from 75％ to 93.6％.No nursing defects or accidents such as patient falls occurred.Conclusion Quality improvement of wearable nurse call system can shorten nurse's response time and increase patient satisfaction.

Objective To explore the best sampling position of bone marrow biopsy in mice.Methods Mice were sacrificed by cervical dislocation, then take the skull, sternum, tibial, femoral and iliac bone, making pathological section.Observed and photographed under light microscope. Comparison of the distribution of the essence and the hematopoietic microenvironment in different parts.Select site which can best reflect the hematopoietic function of bone marrow.Results A large number of hematopoietic cells in ilium marrow sections visible.The cells are evenly distributed. Blood and megakaryocytes were clearly visible.The arrangement of the structure of the scaffolds for tissue closely.The number of fat cells less.Bone marrow hematopoietic cells in the skull, sternum, tibial, femoral were not clear and not active.And there are more fat cells.Conclusions As the best sampling position of bone marrow biopsy, is ilium.

Objective To explore a novel surgical treatment for chronic suppurative otitis media (CSOM) and evaluate its treatment effect.Methods All 97 patients with chronic suppurative otitis media were chosen to be treated using this new surgical method.The skin of the external auditory canal was maintained intact.Open radical mastoidectomy was used to complete clean-up lesions ; the fascia of pedicled temporalis myofascia (PTM) was used to repair the tympanic membrane.The pedicled temporalis fascia,pedicled postauricular periosteal flap and intact skin of the external auditory canal were used in reconstruction of the posterior wall of external auditory canal.Pure tone audiometry (PTA) was performed before and after surgery,recording the air conduction and bone conduction thresholds at 0.5 kHz,1.0 kHz,2.0 kHz,4.0 kHz.The average of the patient's air and bone conduction hearing thresholds was recorded at the 4 frequencies.External auditory canal gauze was removed 3 weeks after surgery.All subjects were followed up for over 2 years.Comparison of hearing thresholds (PTA) was made ① Before and 4 weeks after surgery.② Before and 2 years after surgery.Hearing function comparison include air conduction (AC),bone conduction (BC) and air-bone gap (ABG) analysis.SPSS 16.0 was used in statistical analysis.Pre-and postoperated AC,BC and ABG were compared using T-test.P ＜ 0.05 was considered statistically significant.Results The healing rate of post-operated tympanic membrane was 95.88％ (93/97).Ninty-six ears had 2-year follow-up,and 1 patient was lost in follow-up.There were 2 patients presented with eardrum perforation during the follow-up,and the 2-year healing rate of tympanic membrane perforation was also 93.85％ (92/97).In 96 ears with 2-year followed-up,the average of pre-AC was (52.10 ±3.96) dB,the average of post-AC was (35.67 ±2.52) dB; the average of preABG was (36.6 ± 5.2) dB,and the average of post-ABG (± SD) was (12.14 ± 6.20) dB.Statistical analysis showed significant difference between preoperative and postoperative AC or ABG values (P ＜ 0.05).Conclusion The present surgical procedure broke through the existing conventional mastoidectomy of making a surgical incision in the posterior wall of the external auditory canal.This procedure cleared the lesion completely and preserved the physiological function of the external auditory canal.The acoustic systems and state of gasification to the mastoid tymnpanum were reconstructed,rehabilitated and maintained.The healing rate of hearing and tympanic membrane perforation was improved.

Retrovirus (RV), a major pathogen in acute gastroenteritis in children that may lead to severe disease and heavy burdens on national economy. There is no speciifc drug treatment for the gastroenteritis caused by RV, and vaccine has been identiifed as the most effective intervention to control the associated disease burden. The RV vaccine showed differential effects on different RV infection. In China, epidemiology of RV is more complex hence demands more epidemiologic studies. In this article, we will review the epidemiology of RV in China and further discuss the importance of generalized RV vaccine.