Objective To study on glutathione and glutathione-related enzymes of Antarctic ice microalgae.Methods Glutathione(GSH) content,glutathione produce ability(GPA) and glutathione reductase(GR) activity in 24 kinds of Antarctic ice microalgae were assessed using the spectrophotometer methods.Results GSH content of Cyanophyceae B-1 was highest.Total GSH yields of Antarctic ice microalgae Chlamydomonas sp.ICE-L and Berkeleya rutilans GJ01 were in the top two positions.GPA in most Antarctic ice microalgae was higher than that in mesophilic green algae,and only in two ice microalgae was lower than that in Phaeodactylum tricornutum.GR activities of Antarctic ice microalgae Berkeleya rutilans GJ01 and Chlamydomonas sp.ICE-L were greater than that of the control microalgae.Conclusion It is possible that Antarctic ice microalgae will become a new resource of GSH.