Objective To explore the clinical value of double collagenase injection in treating patients with lumbar disc herniation. Methods Thirty one patients with lumbar disc herniation to whom DCI had been applied were analyzed with 15 to 31 months follow-up. Results The therapeutic effects were excellent in 27, good in 3, fail in 1. The total effective rate was 96.7%.Conclusions DCI is a good method in treating lumbar disc herniation with high effectiveness, extensive indications, low complications and profitable clinical application prospectively.

Gastrectomy ; methods ; Humans ; Pylorus ; surgery ; Stomach Neoplasms ; surgery

Objective Beta-amyloid (AB) plaque is one of the most important hallmarks of Alzhe-imer disease (AD). [N-methyl-11C]-[4'-methylaminophenyl]-6-hydroxybenzothiazole (11C-PIB) can have a strong binding potential (BP) of AB plaques in the brains of patients with dementia of Alzheimer type (DAT). This study was to investigate the value of rostral anterior cingnlate cortex (rACC) in diagnosing human AD with 11C-PIB PET imaging. Methods All the subjects were enrolled through ads by Washington University School of Medicine (WUSM) in USA. Clinical dementia rating (CDR) was the practical grading standard for AD. There were 129 cases of normal control (HC) with CDR =0 and 40 cases of AD patients with CDR >0 (but there were only 120 HC cases and 34 DAT patients with data of rACC PIB BP. All 169 cases had PIB BP data of other studied cerebral subregions). Brain subregions were localized with MRI. PET imaging was performed immediately after intravenous injection of11C-PIB. MRI and PET images were aligned and fused with the help of software. BP was calculated using the Logan graphical analysis and the cerebellar cortex as the reference tissue. The distribution characters of 11C-PIB BP in rACC were analyzed and compared with other brain subregions studied by other scholars with the same research cases. The corre-lation analysis, t-test or variance analysis were calculated with SPSS 11.5. Results rACC PIB BP of 120 HC and 34 AD patients was calculated, rACC PIB BP was statistically linear and positively correlated with CDR significantly (BP=0.2865±0.442,CDR=0.143±0.290,r=0.545,P<0.01). There was no sta-tistical difference among the rACC PIB BP of DAT patients (0.6719±0.1545 vs 0.8933±0.0880,inde-pendent-samples t-test in both groups of CDR =0.5and1, indicated t=-1.245,P>0.05), but signifi-cant difference was found between rACC PIB BPs of HC and DAT patients (0.1589±0.0219 vs 0.7370±0.1125, independent-samples t-test in both group of CDR=0 and>0,indicatedt=-7.998, P<0.01). The threshold of rACC PIB BP for differentiating DAT was 0.4592, greater than what could be thought as suffering from DAT with sensitivity 67.65 % (23/34) and specificity 88.33% (106/120). The BP values of both groups partially overlap each other. In all the studied cerebral subregions, the highest 3 subregions in mean PIB BP of DAT patients or in the difference of mean PIB BP between DAT patients and HC group were precuneus, rACC and prefrontal cortices. Conclusion rACC PIB BP can be used to differentiate DAT patients from normal persons; rACC, precuneus and prefrontal cortices are together the most sensitive brain subregions in the diagnosing DAT.

Objective:To investigate the anti tumor effect of C 215 gene transfected tumor vaccine and monoclonal antibody superantigen fusion protein C 215 Fab SEA.Methods:B16 melonoma cells were transfected with the gene encoding a human colon adenocarcinoma cell surface glycoprotein C 215 to produce a tumor vaccine,melanoma models were established in C57BL/6 mice to observe the antitumor effect of the tumor vaccine and the fusion protein C 215 Fab SEA.Results:Tumor growth in mice treated with the vaccine together with the fusion protein C 215 Fab SEA was significantly inhibited compared with the control group(P

Objective:To investigate the anti-tumor effect of C215gene transfected tumor vaccin e and monoclonal antibody-superantigen fusion protein C215Fab-SEA.Methods:B16 melonoma cells were transfected with the gene encoding a human colon adenoca rcinoma cell-surface glycoprotein C215 to produce a tumor vaccine,melanom a models were established in C57BL/6 mice to observe the antitumor effect of the tumor vaccine and the fusion protein C215Fab-SEA.Results:Tumor growth in mice treated with the vaccine tog e ther with the fusion protein C215Fab-SEA was significantly inhibited comp ared with the control group(P＜0.01),while the tumor growth in group treate d wi th the vaccine only and group treated with the fusion protein only were not obvi ously inhibited compared with the controls.Conclusion:The fusion protein C215Fab-SEA could enh ance the effect of C215-transfected tumor vaccine against the growth of C 215-negative tumors.

[Objective] To summarize Prof. YANG Jinkun gastric cancer clinical experience of diagnosis and treatment, in order to promote clinical application. [Method] Through clinical diagnosis, collecting cases, Prof. YANG Jinkun stomach cancer treatment methods are analyzed and summarized, and enumerated case evidence. [Results] Prof. YANG Jinkun offered to poison pixu(spleen deficiency) for the basic pathogenesis of gastric cancer, on the basis of the spleen and the centralizer, and combined disease according to the nature of different pathogenic toxin, chase respectively eliminating phlegm and stasis fights, qingrejiedu, etc of evil spirits, all applications in the west, comprehensive treatment, the clinical good results have been achieved. For medical record satisfactory curative effect. [Conclusion] Gastric cancer experience of diagnosis and treatment of YANG Jinkun is unique, curative effect is distinct, worthy of clinical application and promotion.

Objective To understand the attitude of health workers towards people living with HIV and AIDS(PLWHA) and AIDS knowledge and desire for related training among them in selected hospitals in Beijing.Method Convenience sampling was used to select health workers from 32 hospitals of different levels in 4 districts within Beijing.Anonymous self-administrated questionnaires were used to collect the information regarding AIDS knowledge and desire for relevant training.Results A total of 1 292 health workers completed the questionnaire.They demonstrated a moderate level of general AIDS knowledge,but a low level of knowledge related to AIDS diagnosis and therapy.Of them 39.7% had received specialized AIDS training and 96% had strongly desire for such specialized training.Most health workers sympathized with PLWHA and understood their situation,while some of them were afraid of PLWHA and wanted to have minimal contact with PLWHA.91.3% of the health workers expressed that PLWHA should have the same rights to medical services.At the same time,49.2% of the health workers stated that they would not like to work in hospitals or departments which accepted PLWHA.80% of the health workers expressed concern over acquiring HIV infection in the workplace.52.7% of them thought that they had a risk of becoming infected in the workplace.Conclusion The level of AIDS knowledge among health workers in Beijing needs to be improved.Specialized training to enhance and develop health workers knowledge of prevention,diagnosis and treatment of AIDS is required.Most of the health workers do not discriminate against PLWHA,but they express concern over infection in the workplace.

50 000 bp)was extracted from frozen gastric cancer tissue and their corresponding normal samples and used for RLGS assay.The genome DNA was digested by methylation-sensitive restriction enzyme Not Ⅰ, and labeled by radioisotope ~(32)p,then separated by two-dimensional electrophoresis and autoradiography. Experimental conditions for each step were optimized step by step.DNA fragment sequences for the dots on scanning profile were identified according to the human RLGS database.Results RLGS assay was set up and used to get the RLGS profiles of the representative tested samples successfully.These profiles can display more than 1 200 available dots averagely,the profiles of high quality DNA sample can display more than 1 800 dots which is the average level at an excellent RLGS lab,discrepant dots which had weaken or enhanced signals and their sequence information were obtained.The result can be reproduced.Conclusion The RLGS assay is established,stabilized for detection of DNA methylation of tissue samples.

Continuous electroencephalographic monitoring (CEEG) has been used for clinical monitoring of acute cerebrovascular diseases for nearly 3 decades,but it has not been used as a conventional monitoring approach in clinical practice.In recent 15 years,CEEG technology has made considerable progress,especially in the field of real-time monitoring of acute cerebral ischemia and epilepsy,which has provided a new possible direction for the diagnosis and treatment of acute cerebrovascular diseases.The article briefly comments the application of CEEG in acute cerebrovascular diseases.

Objective To develop the Chinese version of the 35-item Medical Outcomes Study HIV Health Survey (MOS-HIV) and to assess the reliability and validity of MOS-HIV among HIV-infected individuals.Methods Between April and July of 2007,a croas-sectional survey of 758 HIV-infected individuals was conducted.Responses to the MOS- HIV were analyzed for its reliability and validity.Results Pearson's correlation coefficients of items for test-retest relia- bility ranged from 0.46-0.92,all internal consistency reliability coefficients exceeded 0.69,and all items were more strongly correlated with hypothesized scale than any other scale.Eight scales of MOS-HIV were significantly associated with CD_4 cell counts.Conclusions The Chinese version of MOS-HIV offers good reliability and validity and can be used as a tool to assess the quality of life of HIV infected individuals in China,thus providing evidence for further studying AIDS related quality of life in this country.