OBJECTIVE: Aseptic prosthetic loosening is a primary problem of total hip arthroplasty. For this reason, several prosthesis interfaces with different characteristics have been studied, such as ceramic-on-ceramic prosthesis. The purpose of this study wasto investigate the mid- and short-term curative effects of ceramic-on-ceramic total hip arthroplasty in treatment of avascularnecrosis of the femoral head (ANFH) and to evaluate its clinical application value.METHODS: A total of 23 patients (28 hips) with ANFH who received ceramic-on-ceramic non-cement hip prosthesis replacementwere followed up. All hip prostheses were BiCONTACT~((R)) ceramic-on-ceramic hip prostheses. Functional evaluation was assessedaccording to the Harris hip score. Radiographic analysis was performed using the Gruen's zonal method for the stem and the zonal classification of Delee-Charnley for the cup implants.RESULTS: All patients were followed up for an average of 28 months. The mean Harris hip scores ranged from 39.45 points prior to surgery to 88.57 points after surgery, with a difference which was significant (P < 0.05). Radiographically, no obvious prosthesis loosening or subsidence was observed. CONCLUSION: The ceramic-on-ceramic total hip arthroplasty can produce good short-term curative effects in treatment of ANFH.Radiographic findings do not show obvious prosthesis loosening.

Objective To compare the application effects of pentobarbital sodium ,urethane and midazolam combined with ket-amine/Sumianxin Ⅱ in rabbit bone defect repair .Methods 120 healthy male adult rabbits were randomly divided into three groups according to different anesthetic agents ,40 cases in each group .The group A was intramuscularly injected by pentobarbital sodium , the group B was given urethane by the ear vein and the group C was given midazolam combined with ketamine/and Sumianxin Ⅱ by intramuscular injection .The onset time of anesthesia ,maintenance time ,breathing rate ,heart rate ,complications ,recovery time ,ad-ditional anesthetic agents and postoperative death rate in 3 groups were recorded respectively .Results The onset time of anesthesia in the group B and C was(4 .53 ± 0 .65) min and(3 .56 ± 1 .31) min respectively ,which were significantly shortened compared with the group A(P<0 .05);the maintenance time of anesthesia in the group B was (152 .12 ± 12 .53) min ,which was significantly short-ened compared with the group A and C (P<0 .05);the recovery time in the group C was (31 .05 ± 5 .73) min ,which was significant-ly shortened compared with the group A and B(P<0 .05) ,the propability of additional anesthetic agents and the postoperative mor-tality in the group C were significantly decreased compared with the group A and B (P<0 .05) .Conclusion The combined anesthe-sia of midazolam and ketamine/Sumianxin Ⅱ has definite effect without any observed intraoperative depressions of breathing rate and heart rate and with high safety ,high controllability and less influence on the long term survival of the experimental animals , which is a more ideal anesthesia method of the rabbit bone defect repair .

Composite Resins ; radiation effects ; Light

BACKGROUND: Traditionally, bee venom can treat rheumatic arthritis,rheumatoid arthritis(RA) and so on, but it has strong side effects. So it has been hoped for a long time that the effective angle component could be screened from bee venom, which can be used for the treatment of arthritis perfectly than bee venom.OBJECTIVE: To investigate whether bioactive peptide from bee venom could inhibit infection of arthritis by regulating immunological function so as to probe into a new treatment for RADESIGN: Completely randomized controlled experimental trial based on experimental animalsMETHODS: A municipal key laboratory of animal biology.MATERIALS: The experiment was carried out in the Chongqing Key Laboratory of animal biology from January 2001 to May 2002. Totally 80 rats of clean grade aged 2 to 3 months old with the body mass of 180 to 200 g were provided by Animal Experiment Center of Third Military Medical University of Chinese PLA. The experimental animal certification number was SYXK1 (army) 2002 -007. The animals were divided into 3 groups: normal control group( 10 cases), arthritis group( 10 cases), bioactive peptide group(30 cases).METHODS: Adjuvant-induced arthritis animal models were used and bioactive peptide were given to the animals by muscle injection to observe the knuckle volume and knuckle index changes.MAIN OUTCOME MEASURES: The effect of bioactive peptide from bee venom on the change of knuckle volume and knuckle index in adjuvant-induced arthritis ratsRESULTS: Ten days after injection of 0. 15 mg for each rat, the volume of the paw was (4.72 ±0. 58) mL and the knuckle index was (4.47 ±0.46) mL,which there was significant difference compared with the control group (P＜ 0. 05).CONCLUSION: P-peptide possibly has certain inhibitory effect on the development of the adjuvant-induced arthritis in Wistar rat, and will possibly be a potential therapeutic drug.

Objectives To compare the hemodynamic effects and ventricular activation sequence associated with the respective pacing of the right ventricular outflow tract (RVOT) and right ventricular apex (RVA). Methods 79 patients with complete atrioventricular block (AVB) or sick sinus syndrome (SSS)to undergo permanent ventricular stimulation either at the right ventricular apex (RVA) (n = 38) or right ventricular outflow tract (RVOT) (n =41) were selected. The left ventricular ejection fraction (LVEF),cardiac index (CI), mitral E/A ratio and QRS duration were recorded before implantation of pacemaker and 6 or 12 months after implantation. Results There were no significant differences in CI , LVEF, E/A between RVOT and RVA pacing(P ＞0. 05). Compared with normal sinus rhythm, the QRS duration was significantly lengthened when pacing at RVOT or RVA (P ＜0. 05). RVA pacing was significantly longer than RVOT in QRS duration (P ＜ 0. 05). Conclusions There was no significant difference with the hemodynamic effect between RVOT and RVA pacing in subject with normal heart function. Compared with RVA pacing, RVOT pacing remained relatively normal ventricular activation sequence.

With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.

Objective To explore the effect of comprehensive experiments on clinical practice of internal medicine nursing in the patients with advanced simulation human Simman3G.Methods Two grades of nursing students were selected by random cluster sampling and divided into the experimental group and the control group.The control group used the traditional clinical probation method to carry out clinical probation teaching in Department of internal medicine,the experimental group used the advanced simulation human Simman3G to conduct a comprehensive simulation of teaching before clinical probation.After the completion of the internship the self compiled questionnaire was given to two groups of students to investigate their opinion on the clinical probation.Then we compared,the semester examination results between two groups.Results In the experimental group,the grade of internal medicine nursing was higher than the control group at the end of the semester,P ＜ 0.05.The results of questionnaire survey showed that there was no significant difference between the two groups in the promotion of theoretical knowledge and the two dimensions of the survey of nursing work environment.In the training of clinical thinking,improve nursing skill,communication skill,and the learning interest.At the great harvest of 5 dimensions of probation,the experimental group of students' recognition degree was significantly higher than the control group,P ＜ 0.01.Conclusion Before clinical probation of internal medicine nursing,the application of high-end analog teaching can significantly improve the effectiveness of clinical probation.

With the application of drug eluting stent implantation in coronary intervention, the safety and effectiveness of surgical treatment of obstructive coronary artery disease have has been improved, while the postoperative complications still appear to be a concern. In recent years, studies have found that in-stent neoatherosclosis may be an important factor in the development of late stent failure. And with the development of imaging techniques, the in-stent neoatherosclosis after drug eluting stent implantation has been further recognized. However, the mechanism of the development of in-stent neoatherosclosis is still not very clear.

Objective To explore the clinical curative effect of locking plate in elderly patients with proximal humeral fractures. Methods Seventy elderly patients with proximal humeral fractures were selected, and all patients were treated with surgical treatment. Among them, application of locking plate was in 35 cases (locking plate group), and application of ordinary steel plate was in 35 cases (ordinary steel plate group). The operation time, intraoperative blood loss, fracture healing time and curative effect between 2 groups were compared. Results The operation time, intraoperative blood loss and fracture healing time in locking plate group were significantly lower than those in ordinary steel plate group: (61.3 ± 5.6) min vs. (78.3 ± 4.2) min, (190.5 ± 11.5) ml vs. (268.5 ± 21.2) ml and (12.6 ± 1.8) weeks vs. (16.5 ± 1.5) weeks, the excellent and good rate was significantly higher than that in ordinary steel plate group: 94.29% (33/35) vs. 74.98% (26/35), and there were statistical differences (P<0.05). Conclusions The curative effect of locking plate in elderly patients with proximal humeral fractures is accurate. Locking plate can be fixed firmly, and it is conducive to early recovery after surgery.

BACKGROUND: Construction of lentivirus vector has the advantages of simplicity,it is regard as the most effective and successful method in transgene therapy. OBJECTIVE: To clone the metastasis suppressor gene KiSS-1 from human normal placenta tissue and construct its lentivirus vector. DESIGN,TIME AND SETTING: The open experiment was performed at the Laboratory of Fujian Normal University from September 2006 to December 2007. MATERIAL: The pNL-IRES2-EGFP vector was conservated by the Laboratory of Fujian Normal University. METHODS: Total RNA was extracted from human placenta tissue. The opening reading frame cDNA of KiSS-1 was isolated by using RT-PCR,and cloned into its lentiviral vector pNL-IRES2-EGFP to construct expression plasmid pNL-IRES2-EGFP-KiSS-1. MAIN OUTCOME MEASURES: Clone objective gene fragment of KiSS-1,restriction enzyme digestion and gene sequencing of the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 were observed in the study. RESULTS: The nucleotide sequence isolated from the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 was confirmed the same as expected by restriction enzyme digestion and gene sequencing. CONCLUSION: The recombinant plasmid pNL-IRES2-EGFP-KiSS-1 has been constructed successfully.