BACKGROUND:Type 1 diabetes melitus is an autoimmune attack characterized by the selective destruction of pancreatic cels. Patients need to rely on external insulin to survive, and there is a lack of specific treatment. OBJECTIVE:To review the source of al kinds of stem cels, differentiation capacity, self-exclusion and existing problems, and to provide the basis for regeneration treatment of type 1 diabetes melitus. METHODS: In order to search relevant article about stem cel therapies for type 1 diabetes melitus from PubMed database (from 2003 to 2015), a computer-based search was performed using the key words of type 1 diabetes melitus, stem cel, stem cel therapy, regeneration treatment, stem-cel differentiation, stem-cel transplantation, immune suppression, insulin secreting cels, islet β-cels, therapeutic effect in English. Finaly, 42 articles were chosen for further analysis. RESULTS AND CONCLUSION:Stem cel therapy for type 1 diabetes melitus has attracted quite a lot of attentions. Restoration of damaged β-cels by exogenous stem cel transplantation is the currently ideal therapeutic options, that is, stem cels cultured under suitable conditions can be induced into islet β-cels that have endocrine function. Through al kinds of research, this paper expounds the source of stem cels, directional differentiation, purification, immune suppression characteristics and existing problems, and it also has a discussion for the occurrence and development of type 1 diabetes melitus so as to select the best scheme of stem cels, which helps to cure the disease.

OBJECTIVE:To probe into the countermeasures to solve the obstacle of the Chinese artemisinin manufacture. METHODS:Market structure theory was applied to analyze the obstacle the Chinese artemisinin manufactures were facing and its reason. Then countermeasures and suggestions were provided. RESULTS&CONCLUSION:Chinese artemisinin manufactures can solve the obstacle by these measures,such as integration of the upper reaches of artemisinin recourses,establishment of artemisinin manufacture association,enhancement of research and development.

OBJECTIVE:To observe therapeutic efficacy and safety of escitalopram and fluoxetine in the treatment of depres-sion. METHODS:106 patients with depression were randomly divided into observation group and control group with 53 cases in each group. Observation group was given escitalopram 10-20 mg,qd,and control group was given 20-40 mg,qd. Both groups were treated with 6 weeks. The levels of IL-2,IL-6,TNF-α and Hcy were observed in 2 groups,and HAMD and TESS were re-corded in 2 groups. RESULTS:Total effective rate of observation group (86.79%) was higher than that of control group (77.36%),with statistical significance(P>0.05);after treatment,the levels of IL-2,IL-6,TNF-α and Hcy decreased significant-ly in 2 groups,the observation group was lower than the control group,with statistical significance (P<0.05). After treatment, emotion,mental anxiety,sleep disorder,cognitive disorder,somatic anxiety score and HAMD total score all decreased significant-ly in 2 groups,the observation group was significantly lower than the control group,with statistical significance(P<0.05). TESS score of observation group was significantly lower than that of control group 3 weeks and 6 weeks after treatment,with statistical significance (P<0.05). CONCLUSIONS:Escitalopram is similar to fluoxetine in the treatment of depression,but escitalopram can improve inflammatory response significantly and decrease the level of Hcy with mild ADR.

Objective To optimize the formulation of sinomenine hydrochloride pulsatile drug delivery tablets by central composite design-response surface method.Methods The tablets containing sinomenine hydrochloride were prepared by dry-compression coating technique.The influence factors included the amount of sodium carboxymethyl starch in core tablets, the ratio of HPMC/carrrageenan,and the amount of matrix materials in coating film.The evaluation parameter was lag time.Experiments were done on the central composite design,and the data were simulated using multi-linear equation and second-order polynomial equation.The possibly optimal formulation was predicted by response surface method.The lag time of the tablets prepared under the optimum condition was compared with the predicted.Results The lag time was simulated using second-order polynomial equation and the regression coefficient was 0.993 7.The lag time in vitro of the tablets prepared under the optimum conditions was about 6 h,then drug released in pulsatilerelease character.Bias between the observed and predicted values of the lag time was within ?4.43%.Conclusion The sinomenine hydrochloride pulsatile tablets could release drug quickly in vitro at the predetermined time.Central composite design-response surface method can be used to optimize the formulation and the model developed in this study proves to be highly predictable.

AIM: To establish chromatography fingerprint of Radix Ophiopogonis. METHODS: HPLC was applied on a Kromasil C_(18) column(5 ?m,4.6 mm?250 mm) with CH_3CN-0.01%H_3PO_4 solution by gradient elution,flow-rate of 1.0 mL/min,and the UV absorbance was monitored at 297 nm. RESULTS: 30 common peaks were picked up. CONCLUSION: This method is reliable,simple and provides a reference standard for the quality control of Radix Ophiopogonis.

Objective:To study the accessibility and the effect of group counseling which aim at the internet-dependence college students in China.Methods:According to the internet dependence standard which was established in the author's former research, chose 24 internet dependence students from Tsinghua University and JiLi University as the experimental group, and another 24 students as the control group.The experimental group and the control group had no difference in the scores of adapted CIAS-R before group counseling.There were a pre-test, a post-test and a tracing-test separately set when the group counseling was started, finished, and had a period of 6 weeks after it was finished.Results:In the post-test and tracing-test, the scores of adapted CIAS-R of experimental group were significantly lower than those of the control group(39.5?8.1/46.4?6.0,F=11.38,P

Objective To assess whether lysyl oxidase like-2 (LOXL2) has a predictive or prognostic value in pulmonary fibrosis mice,and identify the relationship between LOXL2 and transforming growth factor (TGF)-β 1.Methods Male C57BL/6 mice (n=50) were randomly divided into the experimental group (n=40) and controls (n=10).Each mouse in the experimental group was induced by injecting bleomycin into trachea and mice were sacrificed on day 3,7,14 and 28,respectively.The concentrations of LOXL2,TGF-β 1 of the serum and lung homogenates were measured by enzyme-linked immunosorbent assay.One-factor analysis of variance (ANOVA) and nonparametric rank sum test was used for the comparisons between the two groups.Associations between these factors were analyzed by Spearman's test.Results ① The levels of serum and lung homogenates LOXL2 in the experimental group were obviously increased as compared to those in the controls [serum:(56±48) vs (25±23),P＜0.05;lung homogenates:(61±32) vs (22±16),P＜0.05].And in the experimental group,a positive correlation was found between the level of LOXL2 and the radiological index,the pathological score of inflammation and fibrosis.(Serum:r=0.435,0.533,0.335,P＜0.05;lung homogenates:0.675,0.736,0.526,P＜0.05).② The levels of lung homogenates TGF-β 1 in the experimental group were obviously increased as compared to those in the controls [(302±197) vs (64±16),P＜0.05].A positive correlation was found between the level of lung homogenates LOXL2 and the level of lung homogenates TGF-β1 (r=0.520,P＜0.05).Conclusion LOXL2 participates in the pathological process of bleomycin induced pulmonary fibrosis in mice.It predicts pulmonary fibrosis in the preclinical stage and is associated with increased risk for disease progression.The elevated levels of LOXL2 and TGF-β 1 may have some relationship.They both are involved in the disease progression of pulmonary fibrosis.

Acute non-variceal upper gastrointestinal bleeding (ANVUGIB)is one of the most common medical emergencies in China and worldwide. In 2009,we have issued the Guidelines for the diagnosis and treatment of acute non-variceal upper gastrointestinal bleedingin China;however,numerous studies on the diagnosis and treatment of ANVUGIB have been conducted in the past years,and the management of ANVUGIB needs to be updated. Finally,the 2015 version of Guidelines for the diagnosis and treatment of acute non-variceal upper gastrointestinal bleedingwas issued. We hope to provide information and help for understanding the new guidelines by clinicians.

Objective]The article analyzed and summed up the experience of making formula and description on treatment of gastric abscess in Qing Dynasty Zhang Yuqing’s Medical Records, in order to provide reference in treating contemporary similar diseases.[Methods]Through studying the medical cases of this book,the authors selected out 30 cases of gastric abscess for futher analysis and summing up the Zhang Yuqing’s experience of description.[Result]He usually used the methods to cure gastric abscess, i.e. expelling phlegm by Banxi and Hualou,harmonizing stomach and liver by Zuojin pills, removing obstruction in channels by Xufuhua decoction, tonifying spleen by Liujunzi decoction and Weiling decoction.These cases had accurate effect and distinct treatment characteristics.[Conclusion]Zhang had rich experience in treating gastric abscess, those can broaden insight in treating similar diseases by traditional Chinese medicine,and increase clinical efficacy.

Cognitive dysfunction is associated with common symptoms of people with epilepsy, mainly declining in attention, memoryand planning ability to judge. With the change of medical model, cognitive rehabilitation should be comprised in epileptic therapeuticschemes by doctors. The relationship between epilepsy and cognition has attracted more and more attention. This article summarized thecognitive influencing factors in epilepsy itself, treatments and social psychological factors in order to explain the mechanism of the effect ofcognitive function.