Aromatic hydrocarbons are capital environmental contaminations. Pseudomonas alcaligenes NCIB 9867 (P25X) is capable of degrading aromatic hydrocarbons via the gentisate pathway. Biochemical characterization indicated that it has isofunctional enzymes for the mono- and dioxygenase- catalysed reactions. One set of the enzymes is constitutive and the other is strictly inducible. To date, only the genes for the constitutively-expressed gentisate dioxygenase and the downstream enzymes have been cloned. A mutant strain of P25X, designated SNZ28, which had the constitutive copy of the gentisate 1, 2-dioxygenase gene interrupted by a streptomycin/spectinomycin resistance gene cassette, was found to still express gentisate dioxygenase when induced by gentisate. The proteome profiles of P25X and mutant SNZ28, grown in the presence and absence of the aromatic inducer gentisate, were compared after 2D-PAGE. Fifteen distinctive protein spots which were observed only in induced cells of P25X and mutant SNZ28 but absent in non-induced cells of both were further analyzed by MALDI-TOF and Q-TOF. Of the 15 proteins, 12 showed significant sequence similarity to proteins with assigned function in other microorganisms . The identification of protein P4 which showed positive identification to a gentisate dioxygenase from Ralstonia species indicated the putative role of this protein to encode gentisate 1, 2-dioxygenase in P.alcaligenes.

OBJECTIVE: To summarize the approaches and methods for clinical pharmacists to provide clinical pharmaceutical care.METHODS: By reviewing pertinent literature combining the working experience of the clinical pharmacists,the approaches and methods for clinical pharmacists to provide pharmaceutical care were explored.RESULTS: Pharmacists could provide clinical pharmaceutical care by participating in clinical rounds,consultation and pharmaceutical monitoring,intervening medical order,offering drug-counseling service,handling the adverse clinical pharmacy events and providing medication education etc.CONCLUSIONS: Clinical pharmacists can provide various kinds of clinical pharmaceutical services for the safe,effective and economical use of drugs.

OBJECTIVE:To investigate the status quo and rationality of the perioperative use of drugs in patients with cataracta. METHODS:The drug use data of 60 patients undergoing cataracta surgery in our hospital from Apr. 2007 to Mar. 2008 were analyzed retrospectively and statistically. RESULTS:The drugs used during perioperative period consisted of antibacterials (applied either systemically or locally) and other assistant drugs (blood coagulants,drugs for decreasing intraocular pressure,anti inflammatory drugs and Chinese patent medicines). All the patients were treated with systematic antibacterials for prevention medication. The systemically used antibacterials consisted of ?-lactam antibiotics,clindamycins,aminoglycosides,fluoroquinolones and amphemycins. The problems existed in systemic use of the antibacterials manifested as irrationality in the choice of drug category,dosage,administration frequency,administration time and drug combination,etc. The locally applied antibacterials consisted of aminoglycosides,fluoroquinolones,tetracyclines and lincomycins. The problems existed in the local application of the antibacterials manifested as irrationality in administration time,drug change and drug combination,etc. CONCLUSION:The rationality of the perioperative drug use in patients with cataracta in our hospital remains to be improved.

OBJECTIVE:To analyze the problems existing in the use of thiamphenicol for outpatients. METHODS:In this retrospective study,a total of 743 outpatient prescriptions of Thiamphenicol enteric-coated tablets in one hospital during Mar. 2008 were analyzed in respect of the prescription writing,medication suitability and amount of money of prescriptions,etc. RESULTS:The problems in the use of Thiamphenicol manifested as nonstandard in prescription writing,irrational in clinical drug choice,drug combination,administration and dosage,etc. CONCLUSION:The use of Thiamphenicol in the hospital is somewhat irrational thus remains to be standardized further.

BACKGROUND:Studies have shown that overexpression of myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) is the main reason for early neuronal regeneration failure. OBJECTIVE:To study the effect of human amniotic mesenchymal stem cel s (hAMSCs) transplantation on neural functional recovery of rats with ischemia/reperfusion (I/R) injury. METHODS:Sixty Sprague-Dawley were randomized into sham, I/R, and hAMSCs groups (n=20 per group). Rats in the hAMSCs group were given 1 mL of hAMSCs suspension (1.0×108/L) via the tail vein 24 hours after I/R injury. Rat neurological function recovery was assessed based on behavior changes, as determined by Longa behavioral score, cylinder test, horizontal ladder walking test and limb symmetry test at 1, 3 days, and 1, 2, 3 weeks post transplantation. Cel migration and distribution were observed using immunofluorescence method at 1 day, and 1, 2, 3 weeks post transplantation. MAG and OMgp protein expression was detected by western blot assay at 2 weeks post transplantation. Neuronal apoptosis was detected by TUNEL staining at 3 weeks post transplantation. RESULTS AND CONCLUSION:In the hAMSCs transplantation group, red marker-positive cel s were visible around the injury region at 1 week after transplantation, and over time, these cel s were increased in number. Significant improvement in the neurological function of rats were observed in the hAMSCs group as compared with the I/R group at 3 days and 1, 2, 3 weeks after transplantation (P<0.05), and the expression of MAG and OMgp proteins were also decreased dramatical y in the hAMSCs group (P<0.05). After I/R injury, the number of apoptotic cel s was increased, but hAMSCs transplantation reversed this effect. Overal , hAMSCs transplantation can reduce neuronal apoptosis by reducing MAG and OMgp expression levels, and thereby promote neurological functional recovery from I/R injury in rats.

Lactoferrin(LF) belongs to the transferrin family and is a multifunctional glycoprotein with a variety of biological activities, which not only binds to viral antigenic determinants to inhibit the recognition and binding of viruses to host cells,but also inhibits cell carcinogenesis through the production of thiobarbituric acid, malondialdehyde and other substances with strong antioxidant properties. In recent years, it has been found that in addition to its antiviral and anti-inflammatory functions, LF also has the ability to promote the repair of various types of damaged tissues. This paper mainly reviews the repairing and pro-repairing effects of LF after skin, muscle, bone, intestinal, brain and gingival injuries, aiming to elucidate the further application prospects of LF in the medical field.

Objective To observe the clinical efficacy of S-1 plus oxaliplatin (SOX) in neoadjuvant chemotherapy for patients with local advanced gastric carcinoma.Methods Clinicopathological data of 68 patients with local advanced gastric cancer were retrospectively analyzed and enrolled as treatment group.All the patients in the treatment group achieved 3-4 cycles chemotherapy of SOX regimen preoperatively.Outcomes of tumor remission after neoadjuvant chemotherapy were evaluated according to RECISI1.1 criteria.Meanwhile,70 patients who underwent operation directly after pathological diagnosis of local advanced gastric cancer during the same period were recruited as control group.Resection rate,R0 resection rate,postoperative complications rate and postoperative progression free survival(PPFS) time were compared between the two groups.Results In the treatment group,after neoadjuvant chemotherapy,4 cases (5.9％) achieved complete renission,36 cases (52.9％) achieved partial remission.Meanwhile,the numbers of cases with stable disease and progressive disease were 15 cases(22.1％) and 13 cases (19.1％) respectively.The objective remission rate and disease control rate in the treatment group were 58.8％ and 80.9％ respectively.The tumor resection rate,R0 resection rate,postoperative complications rate in the treatment group were 92.6％,85.3％ and 14.7％ respectively.The R0 resection rate in the treatment group was higher than that in the control group,the difference was statistically significant(x2 =8.042,P ＜ 0.05).However,the differences of tumor resection rate and postoperative complications rate between the two groups were not statistically significant (x2 =1.711,0.673,all P ＞ 0.05).The median time of PPFS in the treatment group and the control group was 36 months and 30 months respectively,there was statistically significant difference in PPFS between the two groups (x2 =4.049,P ＜ 0.05).Conclusion The neoadjuvant chemotherapy of S-1 plus oxaliplatin for local advanced gastric carcminoma has satisfactory short-term effect and can also improve PPFS.However,further investigation is required to observe the effect of this neoadjuvant chemotherapy regimen on postoperative overall survival.

BACKGROUND:Vascular endothelial injury usually appears following atherosclerosis, hypertension, diabetes mellitus and other diseases, which can be repaired by endothelial progenitor cells (EPCs) in the systemic circulation. OBJECTIVE:To study the effect of salveanolic acid B on the mobilization and adhesion of EPCs in mice. METHODS:Thirty C57BL/6 mice were selected and randomly divided into surgical and non-surgical groups, (n=15 per group). Common carotid arteries of mice in the surgical group were injured by iron wire, then were subdivided into three groups (n=5 per group), and were given the injection of 5% of glucose (control group), 100 g/L salveanolic acid B and 10 μmol/L vascular endothelial growth factor, respectively. Meanwhile, the mice in the non-surgical group received the same assignment and treatment, but the common carotid arteries were not injured. After consecutive 7-day administration, the EPCs in the peripheral blood of the mice were isolated, and the EPCs adhesion was tested by flow chamber experiment. The serum levels of stromal cell-derived factor-1 and interleukin-8 were detected by ELISA kits. RESULTS AND CONCLUSION:The proportion of EPCs in the surgical group was significantly increased compared with the non-surgical group (P < 0.05). After 7-day administration, the proportion of EPCs in the salveanolic acid B and vascular endothelial growth factor subgroups of the surgical group was significantly higher than that in the control subgroup (P < 0.05); the proportion of EPCs in the vascular endothelial growth factor subgroup of the non-surgical group was significantly higher than that in the control and salveanolic acid B subgroups (P < 0.05). Flow chamber experiment revealed that the number of EPCs in the salveanolic acid B subgroup was significantly more than that in the control subgroup (P < 0.05). In the surgical group, the serum levels of stromal cell-derived factor-1 and interleukin-8 in the in the salveanolic acid B and vascular endothelial growth factor subgroups were significantly higher than those in the control subgroup (P < 0.05); in the non-surgical group, the serum levels of stromal cell-derived factor-1 and interleukin-8 in the in the vascular endothelial growth factor subgroup were significantly higher than those in the control subgroup (P < 0.05). To conclude, salveanolic acid B can increase the efficiency of the EPCs mobilization in bone marrowin vivo, help the EPCs adhere to the collagen surface, and upregulate the serum levels of stromal cell-derived factor-1 and interleukin-8.

There is great role of position treta ment in critically paediatric patient.It can not only alleviate the ol cal skin contracture opprse sion,muscle joints,but can cause the redistribution of body fluids. Even changing body position plays a crucial role in a number of specific diseases and treatment modalities. Intensive care unit common position includes seated,semi-seated,supine,semi-supine,left lateral, right lateral op sition na d prone position,anti-trendelenburg position,continuous lateral rotation therapy.The following is a brief introduction to the application of various body positions in the intensive care unit.

OBJECTIVE:To explore the effect of rehabilitation training on restenosis following stent implantation in patients with coronary atherosclerotic heart disease METHODS:A computer.based online search of VIP database was performed to search articles regarding restenosis following stent implantation and rehabilitation treatment for patients with coronary atherosclerotic heart disease,published between January 2000 and October 2009,with key words"coronary artery,stent implantation,intracoronary restenosis,rehabilitation exercise".The data were collected,and the references of each article were reviewed A total of 20 articles were included.RESULTS:Intracoronary stent has become an effective treatment for coronary atherosclerotic heart disease,and intracoronary restenosis has been focused in clinical studies.Prophase treatment following stent implantation is very important for coronary atherosclerotic heart disease.Rehabilitation exercise in combination with antiplatelet anticoagulatiOn and lipid treatment can reconstruct coronary arterial blood transport and significantly reduce incidence of restenosis and thrombus.CONCLUSION:Regular rehabilitation exercise following stent implantation can ameliorate degree of vascular restenosis.