Objective:To investigate the role of nuclear factor-?B(NF-?B)in rats with cerebral ischemia-reperfusion injury. Methods:Rat models of cerebral ischemia-reperfusion were established.24hours later,the expression of NF-?B in rat brain of sham-operation group,operation group and intervention group was detected with immunohistochemistry method,Cytokin-induced neutrophil chemotactics(CINC)in rat brain were measured and histopathological examinations were conducted on the hippocampal CAl.Results:The expression of NF-?B p65 and CINC in the operation group increased significantly compared with the sham-operation group(P

OBJECTIVE:To explore the implementation methods of drug price reform in China. METHODS:Current condi-tion and disadvantages of drug price mechanism in China were analyzed,the problems of the implementation of drug price reform were concluded and summarized,and the implementation methods of drug price reform were put forward. RESULTS & CONCLU-SIONS:Current condition and disadvantages of drug price mechanism in China are mainly reflected as failure of policy for maxi-mum retail price causes part of drugs to be overpriced;government-led centralized procurement of drugs results in that part of drugs are underpriced;the defects in the regulation of drug price addition rate leads to hospitals’procurement of drugs at higher price;government’s excessive interference in drug price affects the fair competition and sound development of pharmaceutical in-dustry. However,there are some problems for drug price reform,including unsound drug distribution system,a lack of fair compe-tition in modern drug market,excessive centralization of power of medical insurance authorities,absent public participation and so-cial supervision,and the“practice of using pharmacy profits to subsidize medical services”leading to wrong medical behavior. These measures were suggested to resolve above problems,i.g. reform the drug distribution system,foster modern drug market,es-tablish a medical insurance authorities-led mechanism for drug price negotiation in which multiple interest parties participate,and develop drug retail enterprises gradually replacing the outpatient pharmacies in hospitals to separate medical services from pharma-ceutical services.

Objective:To study the effect of roadside dust inhalable particulate matter on airway inflammation in rats.Methods:Particles at roadside were collected,analyzed and PM10-treat model of rats were established.Lung tissue histopathology was observed.The counts of total leukocytes and neutrophils in bronchoalveolar lavage fluid(BALF)and superoxide dismutase(SOD),glutathion peroxidase(GSH-Px),malondiadehyde(MDA),cytokin-induced neutrophil chemotactics(CINC)in lung homogenate were measured.Results:The counts of total leukocytes,macrophages and neutrophils in PM10-treat group were increased significantly than those in control group(P

Objective: To investigate the effects of the tandem repeat polymorphisms in the enhancer region (ER) of thymidylate synthase (TS) gene and the 6-bp deletion/insertion (del6/ins6) polymorphism in the 3′ untranslated region (3′-UTR) of TS gene on the clinical outcomes of gastric cancer patients treated with 5-FU-based adjuvant chemotherapy. Methods: One hundred and sixteen patients with gastric cancer were treated with 5-FU-based adjuvant chemotherapy. The polymorphisms of TSER and TS 3′ -UTR in those patients were determined by polymerase chain reaction (PCR) method. Results: Of the 116 patients, the frequencies of the TSER 2R/2R, 2R/3R and 3R/3R were 7.8% (9/116), 31.9% (37/116), and 60.3% (70/116), respectively; the frequencies of the TS3′-UTR ins6/ins6, ins6/del6 and del6/del6 were 9.5% (11/116), 41.4% (48/116) and 49.1% (57/116), respectively. The median survival period in ins6/ins6 carriers was significantly shorter than that of del6/del6 (P = 0.017) or ins6/del6 (P = 0.022) carriers. There was no significant difference in median relapse-free survival period between different TSER carriers (P > 0.05). COX multivariate analysis showed that the ins6/ins6 carriers had increased death risk (P <0.05) compared to the other two genotypes. The median no-recurrence survival period had no statistical difference between them. Conclusion: The polymorphism of TS 3 UTR ins6/del6 may be an independent factor for the prognosis of gastric cancer patients treated with 5-FU-based adjuvant chemotherapy.

OBJECTIVE: Invastigation of macrolides in the treatment of drug effects in chronic rhinosinusit. METHOD: The 165 patients with chronic rhinosinusit were randomly divided into 80 cases of macrolides drug group and 85 cases of cephalosporins group, and therapeutic effect was observed. The therapeutic effect of macrolides was also observed in refractory chronic rhinosinusit. RESULT: Comparing macrolides group and cephalosporins group,there is not statistically significant (P > 0.05). Treatment with macrolides cefixime tablet ineffective treatment of patients for 3 months, compared before and after treatment was statistically significant (P < 0.01). Treatment with cefixime tablet macrolides ineffective treatment of patients for 3 months, compared before and after treatment was not statistically significant (P > 0.05). CONCLUSION Long-term low-dose applications of macrolides have a good effect in patients with chronic rhinosinusit, particularly for refractory chronic rhinosinusit have a significant effect.
Anti-Bacterial Agents ; therapeutic use ; Cephalosporins ; therapeutic use ; Chronic Disease ; Humans ; Macrolides ; therapeutic use ; Rhinitis ; drug therapy ; Sinusitis ; drug therapy ; Treatment Outcome

Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.

Objective To assess the feasibility of the treatment of unresectable late pancreatic cancer with laparoscopic choledochojejunostomy and gastric bypass. Methods From June 2000 to December 2003, laparoscopic choledochojejunostomy and gastric bypass were successfully performed in 15 patients with unresectable late pancreatic cancer. Endoscopic noso biliary drainage (ENBD) was performed before the operation. Results All procedures were completed laparoscopically. Jaundice and hepatic function of the patients were obviously improved after the bypass. Oral nutrition was recovered after operation. The mean operative time was (100?26) min (range 70-200 min); the mean operative blood loss was (60?15) ml (range 30-120 ml); the bowel function recovery was on the 3rd-5th postoperative day; the average hospital stay was (8.1?0.7) days (range 6-13 days). Incision infection ocurred in one patient. No operative complications occurred in other patients. Conclusion Treatment of unresectable late carcinoma of the pancreas with laparoscopic choledochojejunostomy and gastric bypass aided by ENBD is a minimally invasive technique with less postoperative pain,shorter hospital stay, lower procedure related morbidity,and better oral nutrition. The life quality of patients with late pancreatic cancer can be obviously improved.

Objective To study the effect of misoprostol combined with psychological intervention on the patients with induced abortion.Methods 200 cases with intrauterine pregnancy in the first affiliated hospital of Wenzhou medical university from March 2015 to August 2016 were selected as the research object in this study.They were randomly divided into the control group and the experimental group, 100 cases in each group.After using iodophor to rinse the vagina, 600ug misoprostol were placed in posterior vaginal fornix in the experimental group 2 hours before operation.Before the operation, the nursing staff should inform the patient of the relevant surgical attention points, eliminate the negative emotions such as anxiety and anxiety, and improve the treatment compliance.During the operation, the nursing staff should pay attention to the psychological state of the patients.The control group were given lidocaine locally.The clinical efficacy in the groups were compared.Results After treatment, the analgesia obvious effective rate in the experimental group was 38%, the analgesia effective rate was 52%.The analgesia obvious effective rate in the control group was 16%, the analgesia effective rate was 49%.The analgesic effect in the experimental group was significantly higher than that in the control group, with statistical significance (P<0.05).After treatment, the effective rate of cervical dilation in the experimental group was 45%, the effective rate was 66%.The degree of cervical dilatation in the experimental group was significantly better than that in the control group.The amount of intraoperative bleeding was significantly less than that in the control group, with statistical significance (P<0.05).Conclusion Misoprostol combined with psychological intervention can improve the clinical effect of artificial abortion to a large extent, relieve the pain of patients and shorten the operation time.

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many diseases. In this study, we explore the levels of miR-192 and zinc ifnger E-box binding homeobox 2 (ZEB2) in CRC, the clinical signiifcance of miR-192 and the correlation between the expression of miR-192 and ZEB2 protein. Methods: The expression levels of miR-192 and ZEB2 mRNA in 30 colorectal carcinoma samples, 30 corresponding cancer-adjacent tissue samples (from the edge of tumor≥5 cm), 25 colorectal adenoma samples, and 15 normal tissue samples were quantiifed using quantitative real-time polymerase chain reaction (qRT-PCR). ZEB2 protein expression was determined using Western blot. The relationship between the miR-192 and clinicopathological factors, miR-192 and ZEB2 protein expression was analyzed. Results:Signiifcant upregulation of miR-192 expression and reduction of ZEB2 mRNA and protein expression were identiifed in CRC tissues, compared to cancer-adjacent tissues, colorectal adenoma samples, and normal tissues (P<0.05). Low miR-192 levels were signiifcantly associated with lymph node (P=0.021) and distant metastasis (P=0.023). An inverse relationship between miR-192 and ZEB2 protein expression was identified in CRC group (r=-0.365, P<0.05). Conclusion: MiR-192 downexpression was correlated with CRC metastasis. MiR-192 may play a role in the development and progression of CRC through ZEB2.

Objective To investigate the imaging characteristics of pulmonary mucosa-associated lymphoid tissue(MALT)lym-phoma,in order to improve the diagnosis of this disease.Methods X-ray and CT features of 1 9 cases of pulmonary MALT lympho-ma confirmed by operation or biopsy were retrospectively analyzed.Results Among 1 9 cases,there were 4 cases with a single nod-ule,2 cases with a single consolidation,5 cases with multiple consolidations,2 cases with a single patchy shadow,4 cases with patchy consolidations,1 case with multiple patchy shadows combined with irregular cavities,and 1 case with diffused interstitial changes.Grossus air bronchogram was found in 1 1 cases with nodules,consolidations and patchs.Irregular margin was found in 1 5 cases.Spiculation was found in 3 cases.Pleural retraction was found in 2 cases.Pleural effusion was found in 3 cases.Conclusion Pulmonary MALT lymphoma can be showed as solitary nodule,patchy shadow,consolidation,cavity and diffused interstitial change.Pulmonary MALT lymphoma should be considered if such lesions combined with grossus air bronchogram,irregular mar-gin,spiculation or plural retraction..