Objective:To investigate the effect of inhibition of vascular endothelial growth factor(VEGF) antisense oligodeoxynucleotides(ASODN) on human bladder carcinoma in nude mice mediated by ultrasound microbubble. Methods:The mice were randomly divided into 3 groups when transplantation tumor was established: For UM+ASODN group, mixture of microbubble and VEGF-ASODN was inject into every mice from caudal vein plus ultrasound tumor local irradiation. For UM group, microbubble was inject into very mice from caudal vein plus local irradiation with the same condition. For control group, physiological saline was inject into every mice from caudal vein.The treatment was performed every 3 days with total 5 times.The volume change of mouse subcutane tumors was observed.Evaluate the expression of VEGF and CD34 and Ki67 with immunohistochemistry dyeing and calculate the microvessel density (MVD) and cell proliferation index;TUNEL assay was used to analyse the apoptosis of tumor cells. Results:The growth speed of nude mice tumor in UM+ASODN group was obviously slower than control group; The expression of VEGF and The MVD in UM+ ASODN group was lower than control group(P

Objective:To study the inhibiting effect of VEGF antisense oligonucleotides carried by liposome on the growth of human bladder carcinoma in nude mice. Methods:The nude mice model of human bladder carcinoma was established and VEGF antisense oligonucleotides were carried by liposome to be transfected.The cell proliferation index,microvessel density (MVD) and the expression of VEGF were detected with immunohistochmistry streptavidin-biotin peroxidase(SP) method and the cell apoptosis index was determined with Tunnel technology. Results:The growth inhibitory rate in antisense experimental group and sense experimental group were 71.08% and 7.19% respectively. The differences in the cell proliferation index,apoptosis index,microvessel density(MVD) and the expression of VEGF between the antisense experimental group and the sense experimental group or the control group had significances. There was no statistical significance in the detection indexes between the sense experimental group and the control group. Conclusions:The VEGF antisense oligonucleotides can inhibit the neovascularization and the growth of bladder carcinoma in nude mice,which might be an important gene therapeutic strategy for the bladder carcinoma in future.

Objective:To investigate the effect of different depth of anesthesia monitoring in elderly cancer patients in the early postoperative cognitive dysfunction (POCD).Methods: 124 cases were received general anesthesia laparoscopic resection of colorectal cancer in elderly patients, and randomly divided into the observation group and the control group, each with 62 cases. The mean artery pressure (MAP) and heart rate of two groups of patients at different depth of anesthesia in each period were compared with the previous induction of anesthesia (t0), the organ before intubation (t1) and after intubation (t2), before pneumoperitoneum (t3) and after pneumoperitoneum (t4), after surgery (t5) and extubation (t6). The corresponding indexes were also compared between the two groups of patients. Results:In the control group, heart rate of t2, and t4~t6 increased significantly faster. The differences were statistically significant compared with the observation group (t=4.132,t=4.345,t=4.253,t=5.326;P<0.05). MAP parameters in the control group were significantly higher than that in the observation group and the differences were statistically significant (t=5.433, t=4.985,t=5.032,t=5.163;P<0.05). POCD in the observation group was significantly lower than that in the control group and the differences were statistically significant (x2=5.323,P<0.05).Conclusion: In elderly patients with laparoscopic colorectal surgery radical NTS will remain at D2 level, which can effectively reduce the incidence of POCD patients and help patients maintain stable hemodynamics. It is worth of further promoting in clinical.

Biological Therapy ; Carcinoma, Neuroendocrine ; Thyroid Neoplasms ; therapy

Age Factors ; Environmental Exposure ; Humans ; Neoplasms ; epidemiology ; prevention & control ; Research

Objective: To observe the morphological changes of the retina at early stage of streptozotocin (STZ)-induced diabetes mellitus (DM) model, so as to assess the feasibility of using STZ-induced DM as an animal model of early diabetic retinopathy (DR). Methods: Sixty-four male SD rats (body weight [180±20] g) were randomly divided into the CON and DM groups (n=32). Rats in DM group received intraperitoneal injection of 1% STZ once and those in the CON group were given same volume of citrate buffer in the same manner. The average body weight and blood glucose were similar in the two groups before intraperitoneal injection. The blood glucose, body weight, and other parameters were determined once a week after intraperitoneal injection. In the 10 th week, the morphological changes of the retina were observed by H-E staining, stretched preparation of FITC-dextran perfused retinal blood vessels, and transmission electron microscopy in randomly chosen samples. Results: The successful rate of STZ-induced DM model was 100%. The body weight of animals in DM group had no obvious increase after injection, and it even decreased at late stage. The body weight increased gradually in the CON group. The average body weight of DM group ([169.9±26.9] g) was significantly lower than that of the CON group ([439.2±23.5] g) in the 10th week (P<0.001). The average blood glucose of DM group ([26.63±4.54] mmol/L) was significantly higher than that of the CON group ([6.37±0.49] mmol/L) 72 h after injection (P<0.001). DM group had a blood glucose > 16.7 mmol/L and CON group had a blood glucose of 5.6-7.4 mmol/L throughout the 10 weeks. Retina H-E staining showed retinal capillary dilatation and interstitial edema in DM group in the 10th week, and the CON group had no obvious abnormalities. Stretched preparation of FITC-Dextran perfused retinal blood vessels showed vascular tortuosity and caliber irregularity in the DM group, but with no leakage, microvascular tumor or retinal non-perfusion area. The retinal vessel diameter was uniform and the branching was natural and smooth in the CON group. TEM results showed the following retinal ultrastructural changes in the DM group: thicker capillary basement membrane, digitation of capillary endothelial cells, mitochondrial swelling, cristae disruption, and vacuolar degeneration in capillary endothelial cells, bipolar cells and ganglion cells, mitochondrial swelling and cristae disruption in pericytes, decreased membranous disc (photoreceptor cell's outer segment), and widened gap between membranous discs. Conclusion: The retina morphological changes of early stage background diabetic retinopathy (BDR) can be found in the 10th week after STZ injection in rats, and STZ-induced DR model can be used as an early stage DR model; besides, the method is simple, economical, quick, with good reproducibility and high successful rate.

Objective To investigate the protective effect of 17?-estradiol (?E2) on cultured retinal neurons. Methods By using methods of the cultured retinal neurons, the effects of H 2O 2, ? E2, and tamoxifen, the estrogen receptor antagonist, on the cultured retinal neurons were observed. Results H 2O 2 significantly decreased cell viability in cultured retinal neurons in a dose- and time-dependent manner. When the retinal neurons were pretreated with ? E2 30 min prior to H 2O 2 treatment, cell viability was significantly increased (P

The effects of tumor promoter TPA, MZR and PB on gap junction intercellular communication in NIH/3T3 cells were studied using the scrape-loading/dye transfer technique. All of these 3 agents were shown to cause a dose-dependent inhibition of the intercellular communication. The blockage of intercellular communication induced by TPA and MZR was inhibited in the presence of the protein kinase C inhibitor, staurosporine or palmitoyl carnitine, but not inhibited in the casexinduced by PB. The results suggest that the serape-loading/dye transfer technique may be used as a rapid screening assay to detect the tumor promoters and protein kinase C may be involved in the genesis of intercellular communication blockage induced by TPA ane MZR, but not by PB.

Enolase is one kind of important glycolytic enzymes which widely exists in most organisms. A number of recent studies confirm that this enzyme has the functions of activating the plasminogen involving in the processes of infection and mi-gration of parasites reducing the immune function of the host as well as preventing parasites from the immune attack of the host. This paper reviews the current research advances in the parasite enolase and explores its potential for diagnosis drug develop-ment and vaccine target of parasitic diseases.

Small cell lung cancer with brain metastasis is the most common type in brain metastasis tumors with the poor prognosis. Researches show that prophylactic cranial irradiation is not only effective in the prevention and treatment of limited disease brain metastases of small cell lung cancer,but also effective for extensive disease. Moreover,certain medications such as mannitol,nimustine etc also play an important sup-porting role,but there are many other uncertain parts in terms of prevention and treatment remaining controver-sial and needing further research.