Objective To explore the effects of arsenic trioxide and paclitaxel on the expression level of metastasis-associated gene 1(MTA1) mRNA in Caco-2 cells.Methods Methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay was performed to detect the inhibition rate of different concentrations of arsenic trioxide and paclitaxel on the growth of Caco-2 cells,reverse transcription real-time polymerase chain reaction was performed to detect the expression level of MTA1 mRNA.Results With the increasing of the concentrations of arsenic trioxide and paclitaxel,the inhibition rate increased,while the expression level of MTA1 mRNA decreased.The inhibition rate was negatively correlated with the expression levle of MTA1 mRNA(r=-0.636,P<0.05).Conclusion The effects of arsenic trioxide and paclitaxel on the growth of Caco-2 cells and the expression level of MTA1 mRNA could be with dosage-dependence,and the inhibition effects might be negatively correlated with the expression level of MTA1 mRNA.

Objective To investigate the distribution and drug resistance of pathogen in blood culture from 2014 to 2015.Meth-ods 1 260 blood specimens were incubated in our hospital,the positive samples were isolation and identification and the drug resist-ance was analyzed.Results 153 strains of bacteria were isolated from 1 260 blood specimens,and the positive rate was 12.1%. Escherichia coli and Klebsiella pneumonia were the main pathogens in gram negative bacilli,coagulase negative Staphylococci and Staphylococcus aureus were the main pathogens in gram positive cocci,Imipenem and ertapenem were the most effective antibiotics (100.0%)to Escherichia coli and Klebsiella pneumonia,the gram-positive cocci were sensitve to Vanconmycin,linezolid and tigecy-cline.Conclusion Drug resistance of isolated pathogen in blood cultures is very serious.Monitoring the change of pathogens and trends of drug resistance is very important in guiding the clinical use of drug.

Objective To analyze the pathogen distribution and drug‐resistance characteristics in the patients with lung cancer complicating non‐fermentative bacteria lung infection to provide the basis for clinicians to prevent infection and rationally use anti‐bacterial drugs .Methods The clinically submitted respiratory tract specimens in the patients with lung cancer in our hospital from January 2009 to July 2015 were retrospectively analyzed .The isolated pathogenic bacteria were identified by adopting the France Bio‐plum‐Egyptian company Vitek2‐Compact identification instrument ,the drug sensitivity test was conducted by using the K‐B disk diffusion method .The statistical analysis of data was performed by adopting the WHONET 5 .6 software .Results 176 strains of non‐fermentative bacteria mainly came from sputum ,accounting for 80 .1% ,the detection rate of Pseudomonas aeruginosa was highest ,accounting for 48 .2% ,followed by Acinetobacter baumannii and Stenotrophomonas maltophilia ,accounting for 32 .4% and 16 .5% respectively ;the drug susceptibility test results showed that non‐fermentative bacteria had different degrees of resistance to antibacterial drugs or even multiple drug resistance ,in which the resistance of Pseudomonas aeruginosa to amikacin ,tobramycin and cefoperazone/sulbactam ,the resistance of Acinetobacter baumannii to amikacin ,cefoperazone/sulbactam and the resistance of Stenotrophomonas maltophilia to minocycline ,cotrimoxazole and cefoperazone/sulbactam were less than 30 .0% ,which to other an‐tibacterial drugs were more than 30 .0% .Conclusion Non‐fermentative bacteria are common pathogenic bacteria in hospital infec‐tion ,non‐fermentative bacteria isolated from the patients with lung cancer complicating pulmonary infection have serious resistance to commonly used antibacterial drugs ,therefore clinic should strengthen the monitoring of pathogenic bacteria and drug resistance . Cefoperazone/sulbactam is the first choice for treating these bacterial infections .