1.Effect of benzo (a) pyrene on learning behaviors and NMDAR2B and brain-derived neurotrophic factor in hippocampus of weaned rats
Jiwen CHENG ; Youzhen WU ; Jisheng NIE ; Qiao NIU
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(5):394-396
Objective To investigate the changes and mechanism of learning and memory in rats by different doses of benzo (a) pyrene (B(a)P). Methods Forty weaned rats (28 days) were randomly divided into control group (NS), solvent group ( vegetable oil) and three B (a) P dosage groups (the doses were 5,10 and 20 mg / kg body weight respectively ). And all rats were administrated intraperitoneally every other day to one month. The capability of learning and memory in rats were measured by Morris water maze test, and the brain-derived neurotrophic factor ( BDNF) and NMDAR2B content in hippocampus were tested by immunohistochemistry. Results In training of Morris water maze,the average escape latency was extended gradually with increasing dose, and there was a statistically significant difference between high-dose group((62. 78 ±47. 25 )s) and the control group((40.60±38.79)s)(P< 0.01). Compared with the control group(11.25 ±2.63), the number of crossplatform of high-dose group(4.33 ±2.08) was statistically reduced (P<0.05). B(a)P at 10 and 20 mg/kg decreased NMDAR2B and BDNF expression in hippocampus of rats in immunohistochemistry. The level of NMDAR2B was (162.23 ±6.56) in the high-dose group and (150.38 ± 15.34) in the control group(P<0.05);the expression level of BDNF was (163. 13 ± 8.09) in the high-dose group and (141.83 ± 13.37) in the control group(P< 0.05). Conclusion Subacute B(a)P exposure can reduce spatial learning and memory in weaning rats, it may be related to decreased levels of NMDAR2B and BDNF in hippocampus.
2.Relationship between mutated k - ras and biological behavior of colorectal cancer
Wenhui WU ; Longbin XIAO ; Youzhen TANG ; Shirong CAI ; Wenhua ZHAN
Chinese Journal of Pathophysiology 2009;25(11):2159-2162
AIM: To investigate mutations of oncogene k-ras in colorectal cancer tissues and the relationship between mutations of k - ras and biological behavior of colorectal carcinoma. METHODS:The specimens of 123 patients with colorectal cancer were collected. Real - time fluorescence quantitative PCR were performed to detect k-ras mutations at codon 12 and codon 13 of exon 1, and the results were analyzed with the corresponding clinical pathological data. RESULTS: Among 123 colorectal cancer cases, point mutations were detected in 53 cases (40.8% ) , point mutations at codon 12 were found in 42 (34.1 % ) cases, and 11(8.9% ) cases at codon 13.No closely relationship between mutations of k-ras and tumor size, location, invasive depth and differentiation extent was observed. The rate of k-ras mutation in the cases with more invaded lymph nodes was higher than that in the cases without invaded lymph nodes ( P < 0.05 ) , and the rate of k-ras gene mutation in the cases with hepatic metastases was higher than that in no hepatic metastases (P <0.05). The rate of k - ras gene mutation was higher in TNM staging Ⅲ/Ⅳ than that in Ⅰ/Ⅱ( P < 0.05 ). CONCLUSION: Mutation of oncogene k-ras plays an important role in the carcinogenesis and development of colorectal cancer, and it is closely associated with invaded lymph notes and hepatic metastases, suggesting that mutation of k- ras indicates a poor prognosis.
3.The use of percutaneous endoscopic gastrostomy and percutaneous endoscopic jejunostomy in enteral nutrition
Xilong OU ; Songqiao LIU ; Haibo QIU ; Weihao SUN ; Dazhong CAO ; Qian YU ; Youzhen ZHANG ; Ziying WU ; Shunying LIU
Parenteral & Enteral Nutrition 2009;16(6):358-360
Objective: To establish the method of percutaneous endoscopic gastrostomy(PEC) and percutaneous endoscopic jejunostomy (PEJ) for enteral nutrition. Methodes: PEG tubes were placed in 114 patients with Pull method. On the foundation of PEG, PEJ tubes were placed in 26 patients by pushing endoscopy to send tubes through Treitz ligment with usingthe the clip. Results: All PEG insertion was performed successfully. PEJ tubes were placed successfully with a new method in 26 patients. 15 patients had a little blooding and 8 patients had slight infection. 21 patients had respiratory tract infection and had been cured by using antibiotic. There was no severe complication. Conclusion: PEG is simple、safe、efficient. The new method of PEJ is feasible.
4. Using target next-generation sequencing assay in diagnosing of 46 patients with suspected congenital anemias
Yuan LI ; Guangxin PENG ; Qingyan GAO ; Yang LI ; Lei YE ; Jianping LI ; Lin SONG ; Huihui FAN ; Yang YANG ; Youzhen XIONG ; Zhijie WU ; Wenrui YANG ; Kang ZHOU ; Xin ZHAO ; Liping JING ; Fengkui ZHANG ; Li ZHANG
Chinese Journal of Hematology 2018;39(5):414-419
Objective:
To evaluate the impact of the targeted next-generation sequencing (NGS) assay for difficult congenital anemias.
Methods:
Blood Disease Hospital Anemia Panel 2014 (BDHAP-2014) including 217 known genes of congenital anemias was developed. NGS and parental verification were performed for patients who were suspected diagnosed with congenital anaemia from August 2014 to July 2017.
Results:
A total of 46 patients were enrolled in this study, the clinical suspection were 11 cases Fanconi anemia (FA), 8 cases congenital dyserythropoietic anemia (CDA), 6 cases congenital sideroblast anemia (CSA), 12 cases congenital hemolytic anemia (CHA), 1 case dyskeratosis congenital (DC), 4 cases iron-refractory iron deficiency anemia and 4 cases unexplained cytopenia (Uc), respectively. 28 (60.9%) of 46 patients became confirmed cases after targeted NGS, corresponding to 44 mutations of which 33 were new. 26(56.5%) patients with results of the assay matching to clinical suspection, including FA (5/11, 45.5%), CSA (6/6, 100.0%), CDA (3/8, 37.5%) and CHA (12/12, 100.0%). 2 (4.3%) cases not matching to clinical suspection, including dyskeratosis congenital (DC) was made in 1(2.2%) patients with suspected FA and familial hemophagocytic lymphohistiocytosis (FHL) was made in 1(2.2%) patients with suspected unexplained cytopenia (Uc). In 12 CHA patients, the hemolytic type was further clarified by the NGS. The remaining 18 cases were not clearly diagnosed.
Conclusion
Targeted NGS assay is of major impact on congenital anemias. The assay should be used routinely in congenital anemias.
5. The characteristic of hereditary spherocytosis related gene mutation in 37 Chinese hereditary spherocytisis patients
Guangxin PENG ; Wenrui YANG ; Xin ZHAO ; Liping JIN ; Li ZHANG ; Kang ZHOU ; Yang LI ; Lei YE ; Yuan LI ; Jianping LI ; Huihui FAN ; Lin SONG ; Yang YANG ; Youzhen XIONG ; Zhijie WU ; Huijun WANG ; Fengkui ZHANG
Chinese Journal of Hematology 2018;39(11):898-903
Objective:
To reveal the genetic characteristics of erythrocyte membrane protein in hereditary spherocytosis (HS) in China.
Methods:
Next-generation sequencing technology was used to detect mutations in genes of erythrocyte membrane proteins in 51 clinically diagnosed HS patients. The relationship between gene mutations and clinical phenotypes was analyzed.
Results:
Mutations in erythrocyte membrane protein genes were detected in 37 patients, including 17 with ANK1 mutations (17/37, 45.9%), 14 with SPTB mutations (14/37, 37.8%), and 5 with SLC4A1 mutations (5/37, 13.5%). One patient carried both heterozygous ANK1 mutation and SPTB mutation (1/37, 2.7%). SPTA1 and EPB42 mutation was not fou nd in any patient. Nonsense mutations (36.8%) and missense mutations (31.6%) were most common. Of the 38 mutations detected, 34 were novel mutations and have not been reported elsewhere (89.5%). Sixteen HS patients underwent parental genetic validation, 6 patients (37.5%) inherited gene mutation from parents and 10 (62.5%) were
6. Correlation of the degree of band 3 protein absence on erythrocyte membrane by eosin-5′-maleimide binding test and clinical phenotype in hereditary spherocytosis
Guangxin PENG ; Wenrui YANG ; Liping JING ; Li ZHANG ; Kang ZHOU ; Yang LI ; Lei YE ; Yuan LI ; Jianping LI ; Huihui FAN ; Lin SONG ; Xin ZHAO ; Zhijie WU ; Yang YANG ; Youzhen XIONG ; Huijun WANG ; Fengkui ZHANG
Chinese Journal of Hematology 2017;38(6):537-541
Objective:
To investigate the relationship between the eosin-5′-maleimide (EMA) binding test and the clinical severity of hereditary spherocytosis (HS).
Methods:
A total of 258 un-splenectomize HS patients were consecutively enrolled. Correlation of hemoglobin concentration, hemolytic parameters, compensating erythropoiesis and the EMA binding test were evaluated.
Results:
258 (128 male and 130 female) patients were included in this study, including 91 compensatory hemolysis patients, 53 patients with mild anemia, 78 patients with moderate anemia and 36 patients with severe anemia. The median age at diagnosis was 23 (2-70) years. The median decreased fluorescence intensity of EMA binding test was 29.97% (16.09%-47.34%) and the average intensity was (29.70±6.28) % of 258 HS patients. The decreased EMA binding fluorescence intensity correlated with MCV (
7.The clinical and laboratory characteristics of congenital pyruvate kinase deficiency
Lin SONG ; Yuan LI ; Guangxin PENG ; Li ZHANG ; Liping JING ; Kang ZHOU ; Yang LI ; Lei YE ; Jianping LI ; Huihui FAN ; Xin ZHAO ; Wenrui YANG ; Yang YANG ; Yuping ZHAO ; Youzhen XIONG ; Zhijie WU ; Fengkui ZHANG
Chinese Journal of Internal Medicine 2018;57(7):511-513
Clinical data of 19 patients with congenital pyruvate kinase deficiency were analyzed. Insufficient pyruvate kinase confirmed the diagnosis. Laboratory parameters of hemolysis were summarized. In cases of neonatal hyperbilirubinemia and unexplained hemolytic anemia, pyruvate kinase activity and next generation sequencing test may help the early diagnosis.