Objective To investigate the risk of cardiovascular and other target organs damage in the patients with type 2 di abetes mellitus(T2DM) and different body mass indexes (BMI).Methods One hundred and twenty-six patients with T2DM admitted to our hospital from January 2014 to January 2016were prospectively collected,including 57 cases in the study group (BMI≥25 kg/m2) and 69 cases in the control group (18.5 kg/m2≤BMI＜25 kg/m2).The clinical featuresof the two groups were analyzed,and the correlation between BMI and related indexes of target organ damage [carotid arte ry intima media thickness (IMT),arterial stiffness index,coronary heart disease index and glomerular filtration rate] were analyzed.Results There was no statistically significant difference in fasting blood glucose between the two groups (P=0.953),BMI in the study group was significantly higher than that in the control group (P=0.000).When compared with the.control group,the study group showed a significantly increase in age (P=0.038);a significant increase in systolic blood pressure (P=0.000);a significantly increase in diabetic family history (P=0.000);a significant increase in total cholesterol (P=0.012);a significant increase in triglycerides (P=0.000);a significant decrease in high density lipoprotein (P=0.003);a significant increase in HOMA-IR level (P =0.000);a significant decrease in HOMA-β level (P=0.000);a significant increase in IMT level (P=0.000);a significant increasein arterial stiffness index (P=0.000);a significant increase in coronary heart disease index (P:0.001);and a significant decrease in glomerular filtration rate (P=0.000).The Pearson linear correlation analysis showed that BMI was correlated with IMT,atherosclerosis index,coronary heart disease index and glomerular filtration rate,the r values were 0.234,0.257,0.294 and 0.211 respectively(all P=0.000).Conclusion The BMI level in the patients with T2DM is related to cardiovascular and renal function damage.

A dispersive solid phase extraction method combined with reversed phase liquid chromatography was investigated for the determination of benzo ( a ) pyrene ( BaP ) residues in edible oil and oil products. The developed “one-step” sample preparation method integrated with the adsorbent activation, sample extraction and sample cleaning. Using Alumia-N as the adsorbent, good cleaning effect and high recovery ranging from 81. 5% -97. 5% was achieved. In addition, coupled with fluorescence detector (FLD), high sensitivity was obtained with LOD of 1. 0 μg/kg (S/N=3). Furthermore, SPSS was used to design orthogonal optimization experiments and establish the optimal condition. Under the optimized condition, the standard calibration curve was linear over the concentration range of 0. 5-10. 0μg/L, with the regression efficiency (R2) of 0. 9997. The relative standard deviation (RSD) of peak area was between 2. 6%-4. 9%, showing good repeatability and the reliability.

Objective:To evaluate the efficacy and safety of raltitrexed plus oxaliplatin combined with concurrent radiotherapy for advanced esophageal carcinoma. Methods:A total of 54 patients with stageⅡ/Ⅲadvanced esophageal carcinoma according to the clinical staging of esophageal carcinoma nonsurgical methods were treated with raltitrexed plus oxaliplatin combined with concurrent radiotherapy. The patients were irradiated with a dose of 60 Gy in 30 fractions. Two cycles of concurrent chemotherapy were adminis-tered during radiotherapy, with 100 mg/m2 oxaliplatin and 2.6 mg/m2 raltitrexed on d1 and d22. Results:The complete response rate was 16.7%(9/54), and the partial response rate was 68.5%(37/54). The total response rate was 85.2%. The no response and progression rate was 14.8%(8/54). The one-and two-year local control rates and overall survival rates were 75.4%, 57.3%and 70.4%(95%CI, 0.6-0.8), 46.6%(95%CI, 0.3-0.6), respectively. The incidence rates of radiation-induced esophagitis, leucopenia, acute diarrhea, neuro-toxicity were 100%, 72.2%, 16.7%, and 44.4%, of which 7.4%, 7.4%, 1.9%, and 0%were≥grade 3, respectively. Conclusion:Ralti-trexed plus oxaliplatin combined with concurrent radiotherapy can enhance the response rate and prolong the survival of patients with advanced esophageal carcinoma. The regime has mild toxicity and is worthy of further study in PhaseⅢ.

Objective To make a summary of radiation-induced changes in ocular lens and retinal lesions based on dynamic follow-up of a patient exposed to a radiation exposure from 192 Ir source accident,in order to provide the information and experience for the clinical diagnosis and treatment of such injury in emergency of a nuclear or radiation accident.Methods The examination methods included eye lens and visual acuity,slit-lamp examination,fundus examination,automated perimetry,optical coherence tomography (OCT) examination,fundus photography,and fundus fluorescein angiography (FFA).The patient was followed-up for 20 months.Comparative analyses were made of the follow-up results.Results One month after 192Ir radiation exposure,the visual acuities in both eyes were 1.0.Mild age-related opacities were observed in peripheral cortex,but the fundus looked normal.Twenty months later,the patient was followed-up.The visual acuities reduced to about 0.6,evident powder-and punctuate-like opacities could be observed in the cortex of the right lens,and mild turbid foci in the posterior polar and capsule.Multiple scotomata appeared in the superior temporal and inferior nasal in the right eye.The macular fovea thickness was not changed significantly in the right eye.Examination with fundus photography demonstrated mild optical nerve atrophy,drusens,and pale optic discs,while FFA examination showed punctuate or flocculent leakages in both eyes.Conclusions The lens opacity could be induced or accelerated by radiation exposure.In addition to the posterior capsule and pole cataracts,punctuate opacity within cortical layer may also occur.It also suggests that cataract and fundus injuries may occur later during long-term follow-up,even though the eyes may look normal during early examination after the patient exposed to the radiation.

Objective To compare the clinical values of impedance method,optical method and microscopy when used to detect platelet abnormal results.Methods Platelet re-examinations by optical method and microscopy were carried out in case of low confidence degree in platelet test with impedance method by XE-2100 automatic hematology analyzer,and then the results by the three methods were compared.Results Most of the low-confidence-degree results by impedance method could be corrected by re-examination by optical method,and the remained had to turn to microscopy due to unsatisfied requirements of the instrument.Conclusion Optical method has to be involved to correct the platelet abnormal results by XE2100 automatic hematology analyzer,and microscopy should be applied in case optical method doesn't work.The three methods gains advantages and disadvantages of themselves,and can be supplementaries for one another.

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Six crystalline components were isolated from the lipophilic fraction of Artemisia annua L. They have been identified as four sesquiterpenes, one flavonol and one coumarin. Qinghaosu I and III are new sesquiterpenes. Five main constituents, camphene, iso-artemisia ketone, 1-camphor, β-carophyllene, and β-pinene were identified from the volatile oil of this herb.
Artemisia annua ; chemistry ; Artemisinins ; chemistry ; isolation & purification ; Bridged Bicyclo Compounds ; chemistry ; isolation & purification ; Camphor ; chemistry ; isolation & purification ; Monoterpenes ; chemistry ; isolation & purification ; Oils, Volatile ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification ; Terpenes ; chemistry ; isolation & purification

Objective To examine the levels of serum adipunectin (ADPN)in patients with end-stage renal disease (ESRD), and identify the relationship between serum ADPN and tumor necrosis factor (TNF)-α, high sensitivity C-reactive protein(hs-CRP) and renal function. Methods Sixty ESRD patients and twenty normal healthy persons were involved in this study. The patients were divided into three groups: patients with chronic renal failure (CRF, 18 cases), patients with hemodialysis (HD,22 cases) and patients with peritoneal dialysis (PD,20 cases). The levels of serum ADPN, TNF-α and hs-CRP were detected by means of ELISA. Differences in continuous variables be-tween the three groups were evaluated. Results The levels of serum ADPN, TNF-α and hs-CRP in CRF, HD and PD patients were signifi-cantly higher than those in control group ( P<0.01 ). Serum ADPN levels in ESRD patients were positive related to serum TNF-α( r≥0.478, P<0.01 ), and negative related to GFR ( r≥-0.582, P<0.01 ). Conclusion The level of serum ADPN was markedly in-creased in patients with ESRD, and correlated with serum TNF-α level and.renal function.

Objective To investigate a dose response curve based on a genetic workstation with automatic analysis system of dicentric chromosome assay (DCA) for establishing a high speed dose estimation method.Methods Peripheral blood from three healthy volunteers was irradiated in vitro using 60Co γ-rays,and then lymphocytes were cultured and fixed on slides using the standard protocol for DCA.Dicentric chromosome in metaphase cells was analyzed automatically with the genetic workstation and confirmed manually,and the dose response curve of automated dicentric chromosome was fitted.Dicentric chromosome of another peripheral blood sample irradiated with different doses was manually analyzed to verify the accuracy of the above automated DCA.Results The yield of automated DCA was well fitted by an equation Y =0.018 06D2 + 0.012 79D + 0.000 489 1 with a correlation coefficient R2 =0.961.The biological dose of radiation could be accurately estimated by this dose response curve within a few minutes.Conclusions We had successfully established a new dosimetry method by analyzing dicentric chromesome automatically,which can save a lot of manual analysis time and hence has important significance for emergency rescue in nuclear accidents.

Diagnostic Imaging Pediatric bone and joint system was a sub-branch of professional courses.The content was more difficult and learners were not relaxed to master the knowledge alone.It was easy,across time and space,resource sharing and interactive to operate on blackboard teaching platform.We can better accomplish teaching and learning task with pediatric bone and joint diagnostic imaging online course constructed by blackboard platform.