Objective　An animal model in which rats were subjected to 20% TBSA Ⅲ degree burns combined with injection of LPS(1mg/kg B.M.)was used for this experiment.Dynamic changes of platelet-activating factor(PAF),tumor necrosis factor(TNF),xanthine oxidase(XO) and melondialdehyde(MDA) in the intestinal tissue were measured to investigate mechanism and characteristics of the intestinal mucosal damage. The results showed a series of early pathogenic changes in the intestinl mucosa were founded,level of intestinal tissue PAF, XO and MDA level in BCEG was higher than that of any other groups at most times, XO and MDA was more positively correlated with PAF respecively after burns combined with endotoxin injection (P<0.005,P<0.001)，during the peak phase of PAF, intestinal mucosal damage was more evident. It suggests there is synergistic effect on the early intestinal mucosal damage due to activation of mucosal cells,release of PAF and TNF, activation of intestinal epithelial XO, production of free radicals and strengthenment of lipoperoxidation after burns combined with endotoxemia

Glutamine is the most abundant amino acid in mammalian plasma and involved in the metabolism and tissue homeostasis of some important substance under physiological and pathological situations.Function of glutamine should be based on its transport into or out of the functional cells via transporters located in cytomembrane.As the liver is the primary center of amino acids homeostasis in the body,it is helpful to learn structure and function of those transporters in hepatic plasma membrane to having a better understanding of the metabolism and function of glutamine.

This paper digested some papers that were published from January to October,2009,and concerning about Chinese schistosomiasis control and research of schistosome,covering ectopie schistosomiasis,antischistosomal drugs and molluseieides,vector biology,epidemiology,etc.

This paper digested some papers that were published from January to October,2009,and concerning about Chinese schistosomiasis control and research of schistosome,covering schistosomiasis control strategy,schistosome genomies and proteomics,immunodiagnosis of schistosomiasis,molecular biology and immunology,etc.

This review describes the major species,number,breeding pattern,ratio of fenced to freely grazed goat,suscepti?bility to Schistosoma japonicum,pattern of S. japonicum infection,infection rate,intensity of infection,fecal contamination of goat that were bred in 5 marshland and lake provinces of Hunan,Hubei,Jiangxi,Anhui and Jiangsu,and two mountainous provinces of Yunnan and Sichuan,and demonstrates the associations of infected goat distribution with the distribution of infect?ed Oncomelania hupensis snails and humans. Considering the huge number of goat which were predominantly grazed freely in marshland and lake endemic regions of China,the high infection rate,numerous environmental pollution by goat feces,as well as the close correlation between the infected goat distribution and infected snail distribution,goat is considered as a major infec?tious source for schistosomiasis japonica in China,and to play a critical role in the transmission of the disease. Since the control of schistosomiasis in animals is critical to schistosomiasis interruption and elimination,it is suggested that the integrated man?agement of goat schistosomiasis should be included in the national schistosomiasis control program of China.

Schistosomiasis is a neglected tropical disease that severely threatens human health and affects the socioeconomic development. The causative agent that parasitizes in humans mainly involves Schistosoma japonicum,S. mansoni,S. haematobi-um,S. intercalatum and S. mekongi. As the firstly identified schistosome,S. haematobium infection is found to strongly correlate with bladder cancer. This paper mainly reviews the discovery,morphology and life cycle of S. haematobium.

Objective To compare medium-term therapeutic effect by bilateral and unilateral puncture plasma coblation nucleoplasty(PCN) in the lumbar disc herniation patients. Methods 90 cases confirmed no significant rupture of annulus fibrosus of lumbar disc herniation by CT or MRI were randomly divided into two groups. Bilateral and unilateral puncture plasma coblation nucleoplasty groups were respectively divided into group A and group B, and compared the JOA scores. Results 82 patients were followed up for 10 ～ 32 months,the average follow-up time was 16.2 months. Preoperative JOA scores of the two groups were not statistically significant(P ＞ 0.05) but the postoperative follow-up JOA scores were higher than preoperative(P ＜0.05) and the JOA scores of group A were higher than group B(P ＜0.05). Conclusion The medium-term therapeutic efficacy of bilateral puncture coblation nucleoplasty was superior to unilateral therpy.

Objective To analyze the characteristics of TCM syndromes of primary glaucoma to provide evidence for clinical treatment.Methods The disease course, eye pressure and clinical symptoms of 114 patients (120 eyes) with primary glaucoma were studied to analyze their TCM syndrome characteristics and distribution regularities.Results (1) In the primary glaucoma, the patients of liver excess syndrome were at the utmost and those of heart and lung qi deficiency syndrome the least. (2)The disease course of liver excess syndrome group was the shortest and that of spleen and kidney deficiency syndrome the longest (P

Ninty three bp endotoxin binding peptide gene was cloned with PCR from the amino terminal of human LBP cDNA, which was introduced into the expression plasmid Pinpoint Xa 3. After the constructs being transformed into E.coli DH5?, the postive recombinants were identified by restriction enzyme analysis and DNA sequencing. Induced by IPTG, the desired 16.5kD EBP fusion protein was expressed and confirmed with SDS PAGE and Western blotting. These results paved a reasonable way for the study of a new endotoxin binding peptide.

Objective To construct the recombinant adenovirus vector which contained human survivin, then being transfected into dendritic cells. Methods Full length survivin cDNA was obtained from recombinant plasmid pCITE survivin by PCR, the PCR product was double digested with restrictive endonucleases Kpn Ⅰ and Xhol Ⅰ, then being inserted directionally into pAdTrack CMV. The plasmid of pAdTrack survivin was lined with PmeⅠ, the fragment containing survivin was reclaimed and transfected into E coli BJ5183. After having been screened by selected media, the extracted plasmid of positive bacteria was transfected into HEK293 cells with liposome and was identified by observing the green fluorescence protein (GFP) and by PCR method. At the time, the culture media containing virus was transfected into fibroblast, and the expression of survivin was proved by observing the GFP and the Western blot method. Results The recombined adenovirus survivin was constructed successfully and the titer was about 1 65?10 8 pfu/ml. A band was observed by Western bolt and its relative molecular mass was about 16 5kD. Conclusion A recombinant adenovirus vector containing human survivin was constructed successfully, and the survivin protein was expressed by fibroblast. It lays the foundation for further study on survivin as a target antigen for gene therapy