Objective　An animal model in which rats were subjected to 20% TBSA Ⅲ degree burns combined with injection of LPS(1mg/kg B.M.)was used for this experiment.Dynamic changes of platelet-activating factor(PAF),tumor necrosis factor(TNF),xanthine oxidase(XO) and melondialdehyde(MDA) in the intestinal tissue were measured to investigate mechanism and characteristics of the intestinal mucosal damage. The results showed a series of early pathogenic changes in the intestinl mucosa were founded,level of intestinal tissue PAF, XO and MDA level in BCEG was higher than that of any other groups at most times, XO and MDA was more positively correlated with PAF respecively after burns combined with endotoxin injection (P<0.005,P<0.001)，during the peak phase of PAF, intestinal mucosal damage was more evident. It suggests there is synergistic effect on the early intestinal mucosal damage due to activation of mucosal cells,release of PAF and TNF, activation of intestinal epithelial XO, production of free radicals and strengthenment of lipoperoxidation after burns combined with endotoxemia

Sixteen strains of champagne yeasts have deen tested for fermentation characteristies. The results show : C_1, C_3, C, have excellent properties such as high fermentation activity, tiny foam, excellent flocculation and tolerance to alcohol and sulfur dioxide. It is very satisfactory to use them in second fermentation of champagne.

Objective To investigate the feasibility of sclerosing agent injection in the treatment of benign thyroid cysts. Methods Clinical data of 60 patients with thyroid cysts, who had been treated by injection of absolute alcohol or 10% sodium chloride solution, were reviewed retrospectively. Results A total of 74 lesions existed in 60 patients, and 182 times of injection were performed, with a total cure rate of 98 3% (59/60). No complications occurred. Follow-up observations for 1～5 years (mean 2 years) found no recurrence. Conclusions Sclerosing agent injection is a feasible method for benign thyroid cysts, if an exclusion of cystic papillary carcinoma is made.

Ninty three bp endotoxin binding peptide gene was cloned with PCR from the amino terminal of human LBP cDNA, which was introduced into the expression plasmid Pinpoint Xa 3. After the constructs being transformed into E.coli DH5?, the postive recombinants were identified by restriction enzyme analysis and DNA sequencing. Induced by IPTG, the desired 16.5kD EBP fusion protein was expressed and confirmed with SDS PAGE and Western blotting. These results paved a reasonable way for the study of a new endotoxin binding peptide.

Objective To propose the developmental orientation of the science of pathology in PLA in the next five years by reviewing the advances and developmental tendency of pathology in the Eleventh Five-Year Plan. Methods The latest progresses and developmental tendency in pathology were reviewed by reviewing the related reviews and treatises published domestically and abroad. Results With the scientific and technological progresses,especially rapid development of molecular biology,a lot of new knowledge,theories,techniques and methods had been proposed,established and applied in various fields of pathology successfully,which provided a new opportunity for improving clinical pathological diagnosis,pathologic research and teaching,as well as cultivation of academic talents of pathology discipline. Meanwhile,these new advances had also broadened the new field of pathological studies and accelerated the development of military pathology. Remarkable achievements have been obtained in military pathology and oncological pathology,etc. Conclusion Emphasis should hereafter be put on the researches in the fields of molecular pathology,military pathology,clinical pathology and army-civilian common pathological techniques,so as to raise the technical level of pathological diagnosis and perfect the construction of hospital pathology discipline.

Objective To investigate the effects of p53 protein and mRNA expression in rat liver tumor promotion by phenobarbital(PB). Methods Male Wistar rats were randomly divided into 6 groups,i. e. higher dose group,middle dose group,lower dose group,tumor-initiating control group,tumor-promoting group and normal control group. The rat liver tumor DEN-initiating-PB-promoting model was established among higher dose group,middle dose group,lower dose group and tumor-initiating control group. The rats in higher dose group,middle dose group and lower dose group were fed with feed containing 500,100,50 mg/kg PB respectively. The rats in liver-tumor promoting control group were only fed with normal feed. The rats in tumor-promoting group weren't initiated by DEN,were only fed with feed containing 500 mg/kg PB. The rats in normal control groups weren't treated by any factors. At the 1st,5th,10th,15th,20th,30th week of the exposure to PB,the expression of p53 protein and p53 mRNA of the rats in every group were determined by immunohistochemistry and in situ hybridization respectively. Results The expression of the mutant type p53(mtp53) protein was found in liver tumor-initiated rats which revealed precancerous lesion. The expression of p53 protein of rats increased in higher and middle dose groups,and showed higher levels in lower dose group and liver tumor-initiating control group compared with those of normal control group which didn't variate significantly with the prolongation of period of exposure to PB. The expression of wild type p53 (wtp53) mRNA showed lower levels in rats of normal control group and liver tumor-promoting group,showed higher levels in higher dose group,middle dose group,lower dose group and liver tumor-initiating control group compared with those in normal control group. The expression of wtp53 mRNA decreased in higher dose group and middle dose group,increased a little in lower dose group and liver-tumor-initiating with the prolongation of period of exposure to PB. Conclusion During the promoting stage of rat liver tumorigenesis tumor promotor PB might reduce the expression of wtp53 and induce mtp53 expression,which affected the cell cycle arrest and apoptosis,and might favor clonal expansion of preneoplastic hepatocytes,which promoted the formation of liver tumor.

Objective To explore the pathogenesis of early liver damage after burn combined with endotoxemia in rats. Methods A total of 156 healthy Wistar rats were randomized into burn combined with injection of endotoxin group (BCEG), single burn group (SBG), single endotoxin injection group (SEG), and normal control group (NCG). An animal model of rats inflicted by 20% TBSA Ⅲ degree burn combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes of liver morphology, the hepatic functions, and the localization of liver LBP mRNA were determined by light microscopy and electron microscopy, quantitative analysis, and in situ hybridization(ISH). Results More serious damages in BCEG were found than those in SBG and SEG. ALT activity in BCEG increased significantly at 0 5 h ( P

Objective To explore the transcription pattern of ND1, ND2 and mtTFA gene in the myocardial cells and intestinal epithelial cells of rats after hemorrhagic shock. Methods Total RNA of myocardial cells and intestinal epithelial cells in rats were extracted after hemorrhagic shock. ND1, ND2, and mtTFA gene transcription levels were measured by reverse transcription and polymerase chain reaction (RT PCR). Results During the period from 1 to 2 h after hemorrhagic shock, the ND1 gene transcription levels in myocardial cells in the hemorrhagic shock groups were higher than that in the normal control group, but the levels in intestinal epithelial cells were lower than that in the normal control group. The pattern for the changes of ND2 gene transcription in myocardial cells and intestinal epithelial cells was basically similar. Conclusion There might exist certain tissue differences in the changes of ND1 gene transcripts in myocardial cells and intestinal epithelial cells of rats with hypoxic and ischemic damage.

Objective To explore the role of Tbx3 gene in the occurrence and development of breast cancer. Methods 44 cases of the fresh breast carcinoma tissues and peritumor tissues were collected individually, the gene expression of Tbx3 was detected by RT PCR and the relationship between the changes of Tbx3 gene expression and the related clinicopathological factors of breast carcinoma was investigated. Results Obviously, the expression level of Tbx3 gene in breast carcinoma tissues was higher than that in the peritumor tissues. Furthermore, Tbx3 gene expression level in breast carcinoma tissues was not associated with the tumor size and ages of patients. However, the histological grading of breast carcinoma, tumor infiltrating degree, TNM stagings, and axillary lymph node metastasis were positively correlated with the expression level of Tbx3 gene. Conclusion Tbx3 gene with high expression may promote the occurrence and development of breast cancer and it is also likely a symbol of crucial changes in the development of breast cancer

Objective To construct the recombinant adenovirus vector which contained human survivin, then being transfected into dendritic cells. Methods Full length survivin cDNA was obtained from recombinant plasmid pCITE survivin by PCR, the PCR product was double digested with restrictive endonucleases Kpn Ⅰ and Xhol Ⅰ, then being inserted directionally into pAdTrack CMV. The plasmid of pAdTrack survivin was lined with PmeⅠ, the fragment containing survivin was reclaimed and transfected into E coli BJ5183. After having been screened by selected media, the extracted plasmid of positive bacteria was transfected into HEK293 cells with liposome and was identified by observing the green fluorescence protein (GFP) and by PCR method. At the time, the culture media containing virus was transfected into fibroblast, and the expression of survivin was proved by observing the GFP and the Western blot method. Results The recombined adenovirus survivin was constructed successfully and the titer was about 1 65?10 8 pfu/ml. A band was observed by Western bolt and its relative molecular mass was about 16 5kD. Conclusion A recombinant adenovirus vector containing human survivin was constructed successfully, and the survivin protein was expressed by fibroblast. It lays the foundation for further study on survivin as a target antigen for gene therapy