Objective To study transesophageal three dimensional echocardiography in the postoperative effect evaluation of the mitral valve apparatus.Methods Thirty three patients with mitral valve prolapse were examined by three dimensional echocardiography preoperatively or intraoperatively.Among them 16 patients underwent mitral vavle reconstruction and 17 patients underwent mitral vavle replacement,the location and size of mitral valve prolapse(MVP) were observed in two groups.Postoperative three dimensional echocardiography was performed to show the mechanism of residual mitral regurgitation(MR).Results In the group of mitral valve reconstruction,three dimensional echocardiography showed that all of the patients had posterior mitral leaflet prolapse,the lesion involved two segments in 4 cases,the average area of mitral leaflet prolapse was ( 0.80 ? 1.59 ) cm 2.While in the group of mitral valve replacement,only 3 patients with isolated posterior mitral leaflet prolapse,the lesion involved two segments in 13 cases, the average area of mitral leaflet prolapse was ( 1.39 ? 0.91 ) cm 2 (P

Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.

Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.

Collagen/bone morphogenetic protein composites were prepared with collagen type I sponge and bone morphogenetic protein-2 (BMP-2). The composites were implanted into Latissimus dorsi muscles pouches of rabbits. Samples were studied with ALP staining, Von Kossa staining, HE staining, toluidine blue staining and CD31 histochemical labeling of microvessel. Bony samples were then used to repair mandibular defect. The effects were evaluated by X-ray, compressive strength, economycin fluorescence labeling, HE staining, toluidine blue staining and bone quantity analysis. Bone formation induced by collagen/BMP composites was found as woven bone between 4 and 6 weeks; cartilaginous osteogenesis was the main type of bone formation; microvessels could be seen in the bony tissues; and the bone defects were healed completely 6 weeks after operation. Bone formation induced by collagen/BMP composites in the muscles can be used as a donor to repair the bone defect.
Animals ; Bone Morphogenetic Protein 2 ; chemistry ; therapeutic use ; Bone Regeneration ; drug effects ; Bone Transplantation ; methods ; Collagen Type I ; chemistry ; therapeutic use ; Female ; Implants, Experimental ; Male ; Mandibular Injuries ; surgery ; Muscle, Skeletal ; surgery ; Rabbits ; Random Allocation