Aim Five types of civil Toxoplasma gondii ELISA test kit were used to the detected specimen which proved to be the same, foreign-made test kit was used to examine the positive specimen given by these five types of test kit, as to make comparison about quality of test kit. Results IgG-ELISA A,B kits positive showing rates (sensitive)are all 60％ ,the corresponding rate between A&B is merely 50％ ,the specifics are 35％&90％. C, D,E kits' positive showing rates (sensitive)are 30 ％, 60 ％, 70 ％ the specifice are 75 ％, 54 ％, 87 ％. The result about examining the probes given by A, B, D, E kit :IgG corresponding rates are better, which are all more than 90％ ;IgM corresponding rates are all more than 60％. It turn out that, according to the civil conditions of ELISA test kit, we suggest that besides the quality being improved in, all kinds of test kit should be used combinatively, so as to avoid neglect and mistake on examination, and we should be cautious in examining.