Germination of the seeds of Bupleurum chinense and B. falcatum activated by exogenous hormones of dif ferent concentrations was studied. The hormones used were GA3, 6-BA and IAA. The optimum concentration of the 3 hormones that activated the seed germination of B. chinense was 50?g/ml,while that for B. falcatum was 200?g/ml, 100?g/ml and 25?g/ml respectively. Among the 3 hormones, 6-BA showed the greatest effect on the 2 species of Bupleurum L. 50?g/ml 6-BA raised the germination rate of the seeds of B. chinese from 57.5 % to 84. 5 % and 100?g/ml 6-BA raised the germination rate of the seeds of B.falcatum from 28. 5 % to 76.0%

Unclear clinical orientation in practice has become one of the common problems with the effective application of famous and excellent Chinese herbal drugs, consequently restricting their development. The authors suggest that there are three aspects to be considered in dealing with this problem. Clinical evaluation and orientation of the famous and excellent Chinese herbal drugs is an important task, and it is also a requirement for the internationalization of traditional Chinese medicine. Literature evaluation-pharmacology research-clinical expertise analysis method will establish a theoretical basis and method for post-marketing development of famous and excellent Chinese patent drugs.

Objective Vertebral benign and malignant tumors were intractable in the treatment. Previous treatment methods had their own deficiency. The introduction of percutaneous vertebroplasty brought a breakthrough in the treatment. The initial experience of the treatment of vertebral malignant and benign tumors with percutaneous vertebraplasty was analyzed in this clinical research.Methods and materials The treatment of seven cases of vertebral hemangiomas and 11 cases of vertebral malignant tumors were reported. Unipedicular or bipedicular approaches were used in 17 cases, and in one case of cervical hemangioma, the cervical anterior-lateral approach was adopted. 15-20% of bone cement was mixed and injected into the vertebral lesions and made to distribute and cast in the lesions. Results Good results were got in all the 18 cases. After 0.5-7ml of cement was injected into the lesions. The complete relief was got in 10 cases and sub-complete relief was achieved in 6 cases and medial relief was got in two cases. One to nine months of postoperative follow-ups found no recurrences.Conclusions The treatment of vertebral benign and malignant tumors with percutaneous vertebraplasty was mini-invasive, safe, and effective.

OBJECTIVETo observe the differences in timely effectiveness of immediate analgesia on acute migraine between electroacupuncture (EA) at shaoyang meridian points and non-meridian points.

METHODSThe randomized controlled trial method was adopted. One hundred and ten cases of acute migraine were randomized into two groups. 55 cases in each one. In the observation group, EA was applied to Fengchi (GB 20), Waiguan (TE 5), etc. In the control group. EA was applied to non-meridian points. Only one treatment was given, and the nee dies were retained for 30 min in the two groups. Separately. at 10 time points, named before acupuncture, in 5 min, 10 min. 20 min and 30 min (at the moment of needle removal), 1 h, 2 h, 4 h, 6 h and 8 h after acupuncture, the pain intensity numerical rating scale (PI-NRS) was adopted to evaluate the pain severity and the adverse reac tion was recorded.

RESULTS(1) The effective rate of imnmediate analgesia was 87. 3% (48/55) in the observation group. significantly higher than 52.7% (29/55) in the control group (P<0.01). (2) NRS of each group was re duced at each time point, from 5 min to 8 h after acupuncture (all P<0.01). (3) NRS score at 30 min after acu puncture in the control group was obviously lower than that in the observation group (P<0.05), but the scores in 2 h, 4 h, 6 h and 8 h after acupuncture in the observation group were lower apparently than those in the control group (all P<0. 01). The differences in the scores in 5 min, 10 min, 20 min and 1 h after acupuncture were not significant (all P>0. 05). (4) For the reducing amplitude of NRS score at the same time point after acupuncture, the results in 20 min, 30 min and 1 h in the control group were higher apparently than those in the observation group (all P<0. 05). But, in 2 h, 4 h, 6 h and 8 h, the results in the observation group were higher apparently than those in the control group (all F<0.05). The differences in 5 min and 10 min after acupuncture were not significant (both P>0. 05). (5) In 24 h after acupuncture, the recurrence rate of migraine was 12. 7% (7/55) in the observation group, which was lower obviously than 34. 5% (19/55) in the control group (P<0. 05). The adverse reaction was not found in the two groups.

CONCLUSIONEA at the shaoyang meridian points achieves the definite immediate analgesia effect on acute migraine and presents the time effectiveness of analgesia.

Acupuncture Analgesia ; Acupuncture Points ; Acute Disease ; therapy ; Adolescent ; Adult ; Aged ; Electroacupuncture ; Female ; Humans ; Male ; Meridians ; Middle Aged ; Migraine Disorders ; therapy ; Treatment Outcome ; Young Adult

OBJECTIVE To make clear the germicidal efficacy of a large-sized pressure-stream disinfector for(bedclothes) used in the hospital.METHODS The carrier-ration sterilization experiment and local disinfection(experiment) were used to test the germicidal efficacy of pressure-stream disinfector for microorganism being on the contaminated bedclothes.RESULTS Operating the disinfector at the 105℃ for 5 minutes,its killing rate of Staphylococcus aureus,Escherichia coli and Candida albicans on bedclothes was 100%,the natural killing rate of(surface) of bedclothes exceeded 99.28%.CONCLUSIONS The pressure-stream disinfection is an excellently(effective),economical and environment protective method,it deserves to be used widely.

OBJECTIVE:To establish the quality creteria of Dendrobium denneanum cultivated in Sichuan.METHODS: The reference substance had been isolated and identified.HPLC and TLC were applied for determination and identification respectively.RESULTS: The coumarin control had been successfully isolated and the TLC spots were clear.The linear range of coumarin was 0.096～0.480 ?g with an average recovery of 99.1%(RSD=1.44%,n=6).CONCLUSION: The results can overall reflect the internal quality of D.denneanum cultivated in Sichuan and provide basis for quality evaluation and development of D.denneanum from Sichuan.

OBJECTIVE:To study the chemical constitutes of Ligusticum chuanxiong and to establish the method for the content determination of ligustilide. METHODS:The compounds were extracted and percolated by ethanol. Then the samples were separated using silica gel and identified by 1H-NMR,13C-NMR data.HPLC was used to assay the contents of ligustilide. RESULTS:Three compounds were isolated and their structures were identified as Z-ligustilide,Z-6,8',7,3'-diligustilide and Z,Z'-6,6',7,3'a-diligustilide. The contents of ligustilide were no less than 0.70%.CONCLUSION:The separation method can be used to prepare high-purity ligustilide reference substance. And the determination method can be used for quality control of L. chuanxiong.

0.05).(4) Free pressure of portal vein(FPP) significantly declined at post-operation in the two groups(P

Chinese medicinal materials are likely to be polluted by various kinds of fungal toxins during the plantation,collection,transportation and storage,which results in the possibility that quality,safety and treatment effect fails to meet the requirement of safe medicine.This paper reviewed the domestic and overseas studies on the pollution of fungus and fungal toxins in herbs,and made an introduction to the limit standard of fungus and fungal toxins.This paper discussed the importance to establish the inspection standard of various fungal toxins in Chinese medicinal materials and their limit standards to provide references to medication safety and improvement of quality control standard system of Chinese medicinal materials.

Objective To explore the effects of general anesthesia combined thoracic paraverte-bral block on postoperative pain and fast track single-port video-assisted thoracoscopic surgery (VATS).Methods Thirty patients,including male 20 and female 10,received single-port VATS were randomly and equally divided into two groups:group C received general anesthesia only,and group T received ultrasound-guided thoracic paravertebral nerve block combined with general anesthe-sia.Both groups did not use the patient-controlled analgesia,if insufficient analgesia happened (rest-ing VAS scores>4),than used dezocine intravenously as additional analgesia (a single-dose 5-20 mg, no more than 120 mg per day).The Ramsay scores at 1,4,8,12 h after the surgery and the mechani-cal withdrawal threshold on the day before the surgery,at 4,8,12,24 h after the surgery were recor-ded.The first time of post-operation pain feedback,the consumption of dezocine in the first 24 h after surgery,the incidence rates of side effects,the first time off-bed and the hospital stays were also re-corded.Results Compared with group C,the Ramsay scores at 8,12 h postoperatively in group T significantly decreased (P <0.05),and the mechanical withdrawal threshold at 4,8 h postoperatively significantly increased (P <0.05).The first time of post-operation pain feedback in group T was sig-nificantly longer than group C (P <0.05).The consumption of dezocine in the first 24 h after surgery significantly decreased in group T (P <0.05).The first time off-bed and the hospital stays in group T were shorter than group C (P <0.05).Also,the incidence rates of nausea,vomiting in the first 24 h postoperatively were lower in group T (P < 0.05 ).Conclusion General anesthesia combined with single-injected thoracic paravertebral nerve block can effectively relieve the postoperative pain in pa-tients undergoing single-port VATS,reduce the consumption of opioids in the first 24 h postopera-tively,cutting down the occurring rates of adverse reactions,which was beneficial to early ambulate and shortened the hospital stays.