Objective To evaluate the effects of long-term high-fat diet on skin wound repair in mice, and to explore its related mechanism. Methods A total of 16 ten-week-old C57BL/6J wild-type mice were randomly and equally divided into two groups to be fed a high-fat diet(HFD group)and a standard diet(SD group)respectively for 8 weeks. Then, an 8-mm full-thickness skin wound was created on the back of each mouse by using a biopsy punch. The degree of wound healing was observed, wound healing rate and epithelialization level were evaluated every day. The weight of mice was measured before feeding, after 8-week feeding and on day 14 after the operation. Peripheral blood samples were obtained from these mice for the determination of total cholesterol(TC)and triglyceride(TG)levels after a 12-hour fast on day 14 after the operation. Then, all the mice were sacrificed, and wound tissues were resected from the dorsal skin of mice for a histological study. The two-sample t test was used to compare the thickness of new epidermis in the wound surface, collagen deposition rate in the wound bed, count of new vessels, levels of cell proliferation and degree of inflammatory cell infiltration between the two groups. Results The average weight of mice was significantly higher in the HFD group than in the SD group after 8-week feeding(27.3 ± 0.7 g vs. 21.2 ± 0.6 g, t = 21.98, P < 0.001)and on day 14 after the operation (28.8 ± 0.7 g vs. 23.1 ± 1.1 g, t = 25.22, P < 0.001). Similarly, there was a significant increase in the levels of TC(1.35 ± 0.32 mmol/L vs. 0.99 ± 0.28 mmol/L, t = 2.24, P < 0.05)and TG(4.21 ± 0.41 mmol/L vs. 2.71 ± 0.31 mmol/L, t = 6.49, P < 0.05)in the HFD group compared with the SD group 14 days after the operation. Compared with the SD group, the HFD group showed shorter healing time (13.5 ± 0.5 days vs. 12.6 ± 1.1 days, t = 1.99, P < 0.05), lower thickness of newborn epidermis on the wound surface (47.8 ± 13.8 μm vs. 95.7 ± 13.7 μm, t = 5.68, P < 0.001), decreased number of CD31-positive vessels(8 ± 1 vs. 13 ± 3, t = 4.1, P < 0.001)and count of ki-67-positive cells(21 ± 4 vs. 49 ± 10, t = 3.33, P < 0.001), but increased count of infiltrating macrophages and mast cells (both P < 0.05). No significant difference was found in collagen deposition rate in the wound bed between the two groups (P > 0.05). Conclusion Long-term high-fat diet can affect wound healing and delay skin wound repair in mice.

Objective:To establish the reference range of red blood cell parameters within 24 hours after birth of premature infants with different gestational ages and genders.Methods:According to the inclusion criteria and exclusion criteria, a retrospective analysis was performed in premature infants who were admitted to the neonatal intensive care unit from January 1, 2018 to December 31, 2019. These newborns were delivered in the obstetrics department of our hospital or came from other parts of Jilin Province. All of their radial artery blood were collected within 24 hours after birth. According to the blood examination results, we analyzed reference range of red blood cell parameters of these premature infants.Results:With the increase of gestational age, the number of RBC, HGB, HCT, MCHC gradually increases and the number of MCV, MCH gradually decreases. There are differences in some red blood cell parameters of premature infants with 34 week≤gestational age<37 week between different genders. Compared with boys, the number of RBC, HGB, HCT and MCV in girls were higher. The number of RBC in premature infants with 23 week≤gestational age<28 week and 28 week ≤ gestion age<34 week are 2.58×10 12-5.45×10 12/L and 2.97×10 12-5.86×10 12/L respectively. In the group of premature infants with 34 week ≤gestion age<37 week, the number of RBC in boys is 3.38×10 12-5.83×10 12/L, while the number of RBC in girls is 3.18-5.89×10 12/L. There're no difference in RDW among preterm infants with different gestational ages and genders, which is 14.8%-20.6%. Conclusions:The study established the reference range of red blood cell parameters of 23 w≤gestational age<37 w premature infants within 24 hours after the birth and explored the differences in red blood cell parameters of premature infants with different gestational ages and genders.

In the 21st century, one of the focuses in the field of organ transplantation is the prevention of chronic kidney graft dysfunction (CKGD) and the furtherance of the long-term survival rate. Researches on the mechanism and prevention of CKGD have made progress in the important immune and nonimmune factors of CKGD. Researchers' endeavors to establish the model of CKGD in the animals such as inbreeding pigs; to develop new drugs which are characterized by high efficacy, low toxicity, low cost, and synergy when used together with immune depressants available from natural medicine; and to probe deeply into the mechanism and prevention of CKGD, will be the important aspects in the field of organ transplantation in the 21st century.
Graft Survival ; immunology ; Humans ; Kidney ; blood supply ; physiopathology ; Kidney Failure, Chronic ; prevention & control ; Kidney Transplantation ; adverse effects ; Reperfusion Injury ; prevention & control ; Time Factors

Objective To investigate the role of the p38 MAPK pathway in the formation of cytoplasmic vacuoles .Methods Af-ter treated with Anisomycin ,SB203580 or SP600125 ,images of HepG2 ,LM3 ,QBC939 ,Hela and A549 cells were recorded by light microscopy and taken at a magnification of 400 × .The effects of anisomycin ,SB203580 and SP600125 on the activity of p38 and JNK were measured by Western blot .LM3 and A549 cells were stained with the ER-tracker red and the lyso-tracker red and subjec-ted to confocal microscopy analysis .Results (1)Anisomycin could abolish cytoplasmic vacuolization of HepG2 cells .(2)p38 MAPK activation was responsible for anisomycin-induced cytoplasmic vacuolization abolishment .(3)p38 MAPK blocking initiated cytoplas-mic vacuoles formation in various cancer cell lines .(4)p38 MAPK blocking-induced cytoplasmic vacuoles disrupted the integrity of endoplasmic reticulum .(5)p38 MAPK blocking reversibly induced cytoplasmic vacuoles formation .Conclusion These observations provide direct evidence for a role of p38 MAPK signaling in regulating the formation of cytoplasmic vacuoles .

Mature dendritic cells are potent antigen-presenting cells that initiate primary immune responses, while immature dendritic cells have quite different properties from mature dendritic cells and are tolerance inducer actually. Here we describe the method of using monocyte condition medium to generate dendritic cells of different maturation phases from nonproliferating progenitors in human peripheral blood. The procedure involves two steps. The first step(or priming phase) is to work on a 6-7-day culture of plastic-adherent blood monocyte in medium supplement with GM-CSF and IL-4. The second step (or differentiation phase) requires the exposure to monocyte conditioned medium. Only the dendritic cells generated by the first step are actually immature, with strong immature dendritic cell features such as active endocytosis, the same expression of monocyte marker CD14, and much of the MHC class II still lies within intracellular compartments (MIIC). The second stage dendritic cells have all the features of mature dendritic cell, including a stellate shape, nonadherence to plastic, the expression of dendritic cells restricted marker CD83, and very strong T cell stimulatory function. All of these dendritic cell properties are retained for at least 3 days when the cytokines are removed, suggesting that these populations are stable and terminally differentiated. Since progression from immature to mature dendritic cell is entirely dependent on exogenously added growth factor such as monocyte condition medium, the peripheral blood monocyte may help to harness synchronized population of mature and immature dendritic cells for studies or therapies.
Cell Culture Techniques ; Cell Differentiation ; Culture Media, Conditioned ; chemistry ; Dendritic Cells ; cytology ; Granulocyte-Macrophage Colony-Stimulating Factor ; chemistry ; Humans ; Interleukin-4 ; chemistry ; Monocytes ; cytology

Objective To study the association between H cy,hs-CRP,IMA and transient ischemic attack (TIA).Methods One hundred and twenty-six TIA patients were divided into low risk group (n=42),moderate risk group (n=43) and high risk group (n=43) according to their AB-CD2 score with 20 healthy subjects undergoing physical examinarion served as control group.Their clinical data were recorded and their serum Hcy,IMA and hs-CRP levels were compared.Results The serum levels of TC,TG,LDL,Hcy,IMA and hs-CRP were significantly higher while those of HDL were significantly lower in low risk group,moderate risk group and high risk group than in control group (P＜0.05),in moderate risk group and high risk group than in low risk group (P＜0.05),and in high risk group than in moderate risk group (P＜0.05).The serumlevels of Hcy,hs-CRP and IMA were positively associated with ABCD2 score in TIA patients (r=0.36,r =0.31,r =0.24,P＜0.05) but not associated with each other (P＞0.05).Multivariate logistic regression analysis showed that hyperlidemia and Hcy were the risk factors for TIA (P＜0.05,P＜0.01).Conclusion Serum Hcy,hs-CRP,IMA levels are positively associated with AB-CD2 score.Hyperlipidemia and Hcy are the risk factor for TIA.Measurement of serum Hcy,hsCRP,IMA levels is beneficial to the assessment of TIA.

The clinical features, examination findings and gene results of the newborn diagnosed with congenital dysplasia by the ANK3 gene heterozygous mutation in the First Hospital of Jilin University were retrospectively analyzed.A female newborn at 10 minutes presented for postnatal asphyxia and 10 minutes after resuscitation.She had a special appearance, with little spontaneous breathing, no swallowing, extremely low muscular tension, and no primal reflexes.Amplitude integrated electroencephalogram(aEEG) suggested the burst suppression (BS) background activity, BS (+ ), lower boundary at 2 μV, upper boundary at 50 μV, no sleep awakening cycle, no convulsive seizure, and mechanical brush seen in the original electroencephalogram burst.Severe abnormal aEEG was detected.Gene results suggested 2 heterozygous mutations in the ANK3 gene [c.4183(exon33) C >G and c. 8239(exon37) C >T], which have not been previously reported.This case report for the first time reported the clinical phenotype of the ANK3 gene mutation in the newborn with congenital dysplasia.