Objective To investigate the effect of combined traditional chinese medicine and western medicine in treatment old men with femoral trochanteric fracture. Methods120 patients with femoral trochanteric fracture were separated into two groups.Patients in the experiment group were managed by traditional Chinese treatment besides the operations and patients in the control group received only operation treatment.We observed the patients' time of fracture healing and the life quality of them. ResultsThe cure effect of observe group was higher than that of control group(P＜0.05)and the fracture healing time in the observe group was shorter than that of control group(P＜0.01). ConclusionCombined treatment of traditional Chinese medicine and western medicine was more effective in promoting the physical reconstruction of patients with femoral trochanteric fracture.

Fungal infection of lung has been a common disease.The complicated clinical manifestations make its diagnosis and treatment difficult.The following should be paid attention to.First,different pulmonary mycosis may have very different clinical characteristics,so the diagnostic standard and therapy should be established accordingly.Second,the proven cases of candida pneumonia are decreasing,probably because of the decreasing needs for biopsies.So candida pneumonia seems still common.Third,different types of endemic pulmonary mycosis have distinguishing characteristics.Some cases could be misdiagnosed as cancers or other diseases,which we should be aware of.

OBJECTIVE To study the synergism of imipenem(IMP) combined with azithromyicn(AZM) in the treatment of infection associated with Pseudomonas aeruginosa biofilm.METHODS The inhibitory activity of AZM to glycocalyx(GLX) production was detected with the L-tryptophan method.Viable counts in biofilms treated with AZM combined with IMP were detected with the methyl thiazolyl diphenyltetrazolium(MTT) method in vitro.The tissue cage method was used to establish the animal model of local P.aeruginosa biofilm infection and the synergism of IMP combined with AZM was also studied in vivo.RESULTS AZM could inhibit the production of GLX.When IMP was combined with 1/4MIC or 1/16MIC of AZM viable counts in biofilms were less than they were when IMP was given alone in vitro.When AZM and IMP were administered at the same time viable counts in tissue cage fluids were also less than they were when IMP was administered alone in vivo.CONCLUSIONS AZM could enhance the antibacterial activity of IMP in the treatment of infection associated with biofilm.

To investigate the possibility to enhance resistance of human bone marrow cells to anticancer agents by transfection of mdrl gene. We transferred mdrl gene into human bone marrow cells with plasmid pHaMDR1/A containing human mdrl cDNA by Lipofectin.Pgp,mdrl gene expression product was detected by both flow cytometry and immunohistochemistry.Also,Pgp function was tested by efflux study using rhodamine.The resistance of human bone marrow cells to anticancer agents was assayed by color forming unit culture after exposure to Vincristine,Daunomycin, and Vp16.The results showed that mdrl gene was successfully transferred into human bone marrow cells, and it expressed.Biological activity of Pgp was confirmed by efflux study using rhodamine.Transferred human bone marrow cells possessed resistance to anticancer agents.It suggested that transfection of mdrl gene can increase resistance of human bone marrow cells to anticancer agents.

OBJECTIVE To study the prevalence of atypical pathogens in patients with community acquired pneumonia(CAP) and the distribution of serotypes of Legionella pneumophilia.METHODS A prospective study was performed on 257 consecutive adult patients with CAP between Dec 2003 and Nov 2004.Antibodies of the paired serum samples to Mycoplasma pneumoniae,Chlamydia pneumoniae and Legionella pneumophilia serotypes 1 to 14 were detected.RESULTS The etiology of CAP was identified in 82(31.9%) patients.The distribution of causal agents was as follows:M.pneumoniae 63(24.5%),C.pneumoniae 17(6.6%),and L.pneumophilia 11(4.28%).Serotype 12 of L.pneumophilia was the predominant one,responsible for 21.8%(56/257).CONCLUSIONS Atypical pathogens especially M.pneumoniae have an important role in CAP;serotype 12 of L.pneumophilia is the predominant one in northern area of China.

OBJECTIVE To establish a rat model of pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley(SD) rats and evaluate it.METHODS Two hundred SD rats were divided into 2 groups: the P.aeruginosa group and the control group,P.aeruginosa was embedded in minute seaweed alginate beads by an ejection set with an acuminate hole.Then the beads were inoculated into the rats′ lung through tracheal intubation.RESULTS The bacteriological values: P.aeruginosa was detected from rats of infected groups.Bacterial number was higher than 105CFU/g 3 and 7 days after infection and higher than 103CFU/g 14 and 28 days after infection.The pathological changes showed: 3 and 7 days after infection,lung abscess,edema,and consolidation could be seen from lungs of infected groups.At optical microscopy,alginateP.aeruginosa caused a pronounced inflammatory reaction with polymorphonuclear cells surrounding a bead.Fourteen and 28 days after infection,fibrinous adhesions and granulomas became the major pathological changes.CONCLUSIONS The animal model of pulmonary infection can be established by inoculating P.aeruginosa embedded in minute seaweed alginate beads made by an ejection set with an acuminate hole to SD rats.

Objective　To investigate the possibility of reversion of multidrug resistance in lung adenocarcinoma-resistant cell line A549／R by antisense strategy including mdr1 antisense oligodeoxynucleotide and Ribozyme. Methods　The target points, -9 to ＋6 in mdr1 cDNA sequence and GUC at 880 site of mdr1 mRNA were selected. Phosphorothioate antisense oligodeoxynucleotide and DNA of plasmid expressing anti-mdr1 Ribozyme（pHβApr-1 neo／mdr1-Rb ）corresponding to above target points were transfered into A549／R cell by lipofectin. The expression of mdr1 mRNA and Pgp, cellular rhodamine accumulation and sensitivity to doxorubicin were detected respectively in transfered cells and control cells by RT-PCR, flow cytometry, rhodamine test and MTT. Results　Treatment of A549／R cell with antisense oligodeoxynucleotide and Ribozyme led to decrease of mdr1 mRNA and Pgp expression, increase of rhodamine cellular accumulation, and 20-180 fold increase of sensitivity to doxorubicin compared to control cell. Conclusion　The mdr1 antisense oligodeoxynucleotide and Ribozyme are possessed with powerful effect on reversion of MDR in lung adenocarcinoma-resistant cell A549／R by inhibiting mdr1 transcription, cleaving mdr1 mRNA and decreasing Pgp expression. Antisense strategy is a promising method of reversion of multidrug resistance in lung cancer.

AIM: To evaluate the response of obstructive sleep apnea hypopnea syndrome (OSAHS) to fluoxetine. METHODS: After a baseline polysomnography (PSG) and Epworth Sleepiness Scale (ESS) documented OSAHS, 10 patients were treated with fluoxetine (20 mg?d -1 ) for four weeks, a repeating PSG and ESS was performed to evaluate the action of fluoxetine. RESULTS: After 4 weeks fluoxetine treatment, the proportion of REM sleep time was decreased by 9.2 %, and the assessment of ESS was significantly decreased (P 0.05 ). The apnea/hypopnea index (AHI) fell in average 24.84 per hour,the mean SaO 2 and minimum SaO 2 significantly increased by 3.2 % and 16.6 %, respectively, and desaturation event index significantly decreased 18.24 per hour (P 0.05 ). The treatment efficiency of fluoxetine to OSAHS was 50%. CONCLUSION: Short term use of fluoxetine in patients with OSAHS is associated with better sleep quality, improvement in oxygenation,and reduction of episodic apneas or hypopneas.

OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro.METHODS Formation of bacterial biofilm was examined by crystal violet assay,viable cells counting in biofilms was also carried out.Alterations of biofilms were measured under varying concentrations of moxifloxacin.RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin.The similar results were obtained for viable cell counting.Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L.CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.

0.01), no significant differences were observed. The peak SBA of CPZ/SBT and CPZ/TZBT against ESBLs positive K. pneumoniae and E. coli were shown to be slightly stronger than that of CPZ by 2-4 times (P