Objective To investigate the effect of combined traditional chinese medicine and western medicine in treatment old men with femoral trochanteric fracture. Methods120 patients with femoral trochanteric fracture were separated into two groups.Patients in the experiment group were managed by traditional Chinese treatment besides the operations and patients in the control group received only operation treatment.We observed the patients' time of fracture healing and the life quality of them. ResultsThe cure effect of observe group was higher than that of control group(P＜0.05)and the fracture healing time in the observe group was shorter than that of control group(P＜0.01). ConclusionCombined treatment of traditional Chinese medicine and western medicine was more effective in promoting the physical reconstruction of patients with femoral trochanteric fracture.

Fungal infection of lung has been a common disease.The complicated clinical manifestations make its diagnosis and treatment difficult.The following should be paid attention to.First,different pulmonary mycosis may have very different clinical characteristics,so the diagnostic standard and therapy should be established accordingly.Second,the proven cases of candida pneumonia are decreasing,probably because of the decreasing needs for biopsies.So candida pneumonia seems still common.Third,different types of endemic pulmonary mycosis have distinguishing characteristics.Some cases could be misdiagnosed as cancers or other diseases,which we should be aware of.

OBJECTIVE To study the synergism of imipenem(IMP) combined with azithromyicn(AZM) in the treatment of infection associated with Pseudomonas aeruginosa biofilm.METHODS The inhibitory activity of AZM to glycocalyx(GLX) production was detected with the L-tryptophan method.Viable counts in biofilms treated with AZM combined with IMP were detected with the methyl thiazolyl diphenyltetrazolium(MTT) method in vitro.The tissue cage method was used to establish the animal model of local P.aeruginosa biofilm infection and the synergism of IMP combined with AZM was also studied in vivo.RESULTS AZM could inhibit the production of GLX.When IMP was combined with 1/4MIC or 1/16MIC of AZM viable counts in biofilms were less than they were when IMP was given alone in vitro.When AZM and IMP were administered at the same time viable counts in tissue cage fluids were also less than they were when IMP was administered alone in vivo.CONCLUSIONS AZM could enhance the antibacterial activity of IMP in the treatment of infection associated with biofilm.

To investigate the possibility to enhance resistance of human bone marrow cells to anticancer agents by transfection of mdrl gene. We transferred mdrl gene into human bone marrow cells with plasmid pHaMDR1/A containing human mdrl cDNA by Lipofectin.Pgp,mdrl gene expression product was detected by both flow cytometry and immunohistochemistry.Also,Pgp function was tested by efflux study using rhodamine.The resistance of human bone marrow cells to anticancer agents was assayed by color forming unit culture after exposure to Vincristine,Daunomycin, and Vp16.The results showed that mdrl gene was successfully transferred into human bone marrow cells, and it expressed.Biological activity of Pgp was confirmed by efflux study using rhodamine.Transferred human bone marrow cells possessed resistance to anticancer agents.It suggested that transfection of mdrl gene can increase resistance of human bone marrow cells to anticancer agents.

OBJECTIVE To study the prevalence of atypical pathogens in patients with community acquired pneumonia(CAP) and the distribution of serotypes of Legionella pneumophilia.METHODS A prospective study was performed on 257 consecutive adult patients with CAP between Dec 2003 and Nov 2004.Antibodies of the paired serum samples to Mycoplasma pneumoniae,Chlamydia pneumoniae and Legionella pneumophilia serotypes 1 to 14 were detected.RESULTS The etiology of CAP was identified in 82(31.9%) patients.The distribution of causal agents was as follows:M.pneumoniae 63(24.5%),C.pneumoniae 17(6.6%),and L.pneumophilia 11(4.28%).Serotype 12 of L.pneumophilia was the predominant one,responsible for 21.8%(56/257).CONCLUSIONS Atypical pathogens especially M.pneumoniae have an important role in CAP;serotype 12 of L.pneumophilia is the predominant one in northern area of China.

Objective To study the correlation between mutant selection window (MSW) and selective enrichment of Staphylococcus aureus resistant mutants in vivo. Methods Tuberculosis patients colonized with S. aureus in anterior nares were selected as experimental group. The susceptibility of S. aureus to rifampicin was determined at the beginning of and 2,4 and 5 weeks after the anti-tuberculosis therapy with rifampicin-containing regimens. S. aureus isolates developing acquired resistance were examined by molecular strain typing. Diabetes patients colonized with S. aureus served as an untreated control. Results The S. aureus isolated from 5 patients acquired rifampicin resistance in 58 tuberculosis patients. It was determined by pulsed-field gel electrophoresis and protein A repeat sequence the strains of S. aureus were different, but the isolates obtained from the same patient before and after acquisition of resistance were the same strains. No resistance was acquired in 39 untreated control patients, which differed statistically from treated patients. Conclusion The selective enrichment of rifampicin-resistant S. aureus mutants occurred when rifampicin concentration was in the mutant selection window.

Objective To establish a rat model of chronic pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley (SD) rats, and to evaluate the animal model with bacteriological and pathological stndies. Methods 240 healthy, clean SD rats were randomly divided into 2 groups: the infected group and the control group. Pseudomonas aeruginosa were embedded in minute seaweed alginate beads using an ejection set with an acuminate hole. Then the beads were inoculated into the rats′ lung through tracheal intubation. The bacteria number in the lung and pathological scores were determined 3, 7, 14 and 35 days after inoculation. The control group was treated with the same method using the sterile normal saline instead of the bacteria suspension. Results Bacteriological values: no bacterium was detected in the control group. Pseudomonas aeruginosa was detected from the rats of infected group in which the bacterial number was up over 105cfu/g at 3 and 7 days after infection, and up over 103cfu/g at 14 and 35 days after infection. Pathological changes: at 3 and 7 days after infection, lung edema, consolidation and hemorrhage could be seen in rat lungs of the infected group. Under the optical microscope, alginate-Pseudomonas aeruginosa caused a pronounced inflammatory reaction with polymorphonuclear cells surrounding beads, and microcolonies formed at the periphery of beads were also seen. Atelectasis and fibrosis and granalation were the major pathological changes at 14 and 35 days after infection. While in the control group, there were little changes after the inoculation except mild congestion and inflammatory reaction in the lungs 3 days after inoculation. Conclusion The rat model of chronic pulmonary infection can be reproduced by inoculating Pseudomonas aeruginosa embedded in minute seaweed alginate beads.

AIM: To evaluate the response of obstructive sleep apnea hypopnea syndrome (OSAHS) to fluoxetine. METHODS: After a baseline polysomnography (PSG) and Epworth Sleepiness Scale (ESS) documented OSAHS, 10 patients were treated with fluoxetine (20 mg?d -1 ) for four weeks, a repeating PSG and ESS was performed to evaluate the action of fluoxetine. RESULTS: After 4 weeks fluoxetine treatment, the proportion of REM sleep time was decreased by 9.2 %, and the assessment of ESS was significantly decreased (P 0.05 ). The apnea/hypopnea index (AHI) fell in average 24.84 per hour,the mean SaO 2 and minimum SaO 2 significantly increased by 3.2 % and 16.6 %, respectively, and desaturation event index significantly decreased 18.24 per hour (P 0.05 ). The treatment efficiency of fluoxetine to OSAHS was 50%. CONCLUSION: Short term use of fluoxetine in patients with OSAHS is associated with better sleep quality, improvement in oxygenation,and reduction of episodic apneas or hypopneas.

OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro.METHODS Formation of bacterial biofilm was examined by crystal violet assay,viable cells counting in biofilms was also carried out.Alterations of biofilms were measured under varying concentrations of moxifloxacin.RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin.The similar results were obtained for viable cell counting.Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L.CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.

Objective Determination of the specific antibodies of IgG and IgM against SARS virus in serum specimens from convalescent SARS patients. Methods The contents of IgG and IgM antibodies against coronavirus were determined with ELISA in the blood of 22 convalescent SARS patients. At the same time, the sera from 22 healthy people were used as negative control. Results The positive rate of specific IgG was 100.0% in convalescent SARS patients (the minimal OD value was 0.477, the maximal OD value was 1.851, the mean value was 1.163), within 71 days of recovery. The positive rate of specific IgM was 22.73% in convalescent patients (5 in 22 samples), and it became negative in all patients after 65 days. On the contrary, the IgG and IgM antibodies were all negative in all 22 healthy people. Conclusion The results indicated that all patients convalescent from SARS could generate specific IgG antibody, which might last for a long time.