1.Purification of a New Antibacterial Protein from Housefly(Musca domestica)Larvae
Hui QU ; Youjin HAO ; Yingjun JING ; Desen LI ; Rongqian DU
China Biotechnology 2006;0(09):-
Insects exhibit a particular resistance to infections.The activation of the innate immune response of insects is involved in the recognition of the infectious nonself and subsequent activation of cellular and humoral reactions.In humoral reactions,insect antibacterial peptides and lysozyme are very important in resistance to infections.Housefly(Musca domestica) is one of the most important kinds of insects and it has strong ability to adapt to the adverse circumstances.It is of momentous theoretical and practical significance to research the immunity system of housefly.The methods of inducement of housefly larvae were firstly studied.Then an antibacterial protein,whose molecular weight is 28kDa,was purified from housefly larvae,induced by 30% H2O2,through saltout,Sephadex G25 column,Sephadex G75 column and CMSepharose Fast Flow column.This antibacterial peptide had activities against most of Gram positive and Gram negative bacteria.
2.The value of the fiber laryngoscope in the diagnosis of congenital laryngeal stridor.
Jie CHEN ; Xiaowen MA ; Youjin LI ; Hao WU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(24):1105-1111
OBJECTIVE:
To explore the value of fiber laryngoscope in the diagnosis of congenital laryngeal stridor (CLS).
METHOD:
The clinical data of One hundred and thirty children who had been examen by laryngofiberscopy and had laryngeal stridor were analyzed retrospectively.
RESULT:
We found 73 children having CLS, 2 children having laryngeal web, 5 children having congenital epiglottic cyst, 20 children having mass and cyst of tongue root, 1 child having laryngocele, 3 children having laryngeal papilloma, unilateral or bilateral cricoarytenoid joint of 9 children having poor activities, 17 children were normal.
CONCLUSION
CLS is misdiagnosed easily. Laryngeal strio caused by other diseases soule be excluded before CLS is diagnosed. Our proposal is that fiber laryngoscope should be used as a routine method in the diagnosis of CLS. CT and MRI could also be used if it is necessary.
Child, Preschool
;
Female
;
Humans
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Infant
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Infant, Newborn
;
Laryngeal Diseases
;
congenital
;
diagnosis
;
Laryngoscopy
;
methods
;
Male
;
Respiratory Sounds
;
Retrospective Studies