Purpose:To investigate hypoxia induced apoptosis and anti apoptosis in ovarian cancer cell line SKOV 3ipl cells and its correlation with cell cycle changes.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an Anaerobic system insulated with 85% N 2,5% CO 2,10% H 2 and very low O 2 concentration (100 ppm). Apoptosis and cell cycles were measured with FITC Annexin V/PI double stain and DNA prep stain Flow cytometry in cells cultured in anaerobic condition. Results:Apoptosis in cells cultured in hypoxia condition for 0h,8h,16h,24h. were (0.32?0.01)%,(8.29?0.87)%,(4.23?0.75)%,(0.59?0.08)% respectively. Between 8h or 16h and 0h, P

Purpose:To investigate the adaptation of ovarian cancer cell line SKOV 3ipl cells to hypoxia and its correlation with the expressions of VEGF and Bcl 2 protein.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an anaerobic system consisting of 85%N 2, 5%CO 2, 10%H 2 with very low oxygen concentration (

OBJECTIVETo study the influence of different technique process and prescription on hygroscopic property of pharmaceutical materials of traditional Chinese medecine (TCM) was studied.

METHODThe sample prepared with banlangen and different excipients had been put in certain suroundings for 24 hours, then the hygroscopic curves and their parameters of hydroscopicity were gotten; the influence of that on hygscopic property of banlangen was studied.

RESULTThe optimized damp-proof materials was polyoxylate II. The damp-proof effect enhanced with the increase of temperature and the excipient the decrease of and concentration of ethanol.

CONCLUSIONExcipients have different effect on hyproscopic property of banlangen, and the damp-proof technology has direct effect on it.

Desiccation ; Drug Compounding ; methods ; standards ; Drug Prescriptions ; Drugs, Chinese Herbal ; chemistry ; Ethanol ; chemistry ; Medicine, Chinese Traditional ; Quality Control ; Regression Analysis ; Temperature ; Time Factors ; Water ; chemistry ; Wettability

OBJECTIVETo apply Fuzzy mathematical methods to choose the best taste modifying prescription of oral solution of traditional Chinese drug.

METHODJin-Fukang oral solution was used as a model drug. The oral solution was prepared in different taste modifying prescriptions, whose tastes were evaluated by the fuzzy quality synthetic evaluation system.

RESULTCompound-sweeteners with Sucralose and Erythritol was the best choice.

CONCLUSIONFuzzy integrated evaluation can be used to evaluate the taste of traditional Chinese medicinal pharmaceuticals, which overcame the artificial factors and achieve more objective conclusion.

Administration, Oral ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Fuzzy Logic ; Humans ; Models, Biological ; Sweetening Agents ; pharmacology ; Taste ; drug effects

OBJECTIVETo study the changes of the in vitro and in vivo material base of Shaoyao Gancao decoction (SGD) based on HPLC-DAD-ESI-MS, and confirm the migrational constituents in plasma.

METHODKormasil C18, (4.6 mm x 250 mm, 5 microm with protection column) was employed; acetonitrile and 0.5% acetic acid/water was used as mobile phase for gradient elution, flow rate was set at 0.8 mL x m min(-1), column temperature was 25 degrees C, and methanol was used to remove the protein. A LCQdeca mass spectrometer system equipped with an electrospray ionization iontrap source was used as the detector and operated in the positive ion mode. Ions was scanned from the m/z 100 to m/z 1000, and characteristic ion were secondary schizolysised to obtain data of second order MS.

RESULTThe main constitutes of SGD were peoniflorin, Catechin-5-O-glucoside, albiflorin, liquiritin and glycyrrhizic acid etc, and glucosides were found in the plasma samples of rats administered with SGD, which were glucuronide conjunctions of liquiritigenin and isoliquiritigenin.

CONCLUSIONLiquid chromatography-mass spectrometry technology provides a convenient and high speed method to analyze the migrational constituents of plasma of rats administered with SGD, therefore, it has a good value in studies on the changes in the material base of traditional Chinese medicine (TCM).

Animals ; Blood Chemical Analysis ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; pharmacokinetics ; Male ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Electrospray Ionization ; methods

This paper was aimed to study the moisture adsorption of Chinese herbal medicine ingredients at different environment.The film mass transfer model and Fick's second law were applied to evaluate the moisture diffusion for Chinese herbal medicine ingredients.The results showed that under the temperature of 25℃ and 50％ relative humidity,the diffusion coefficient of 13 medicine ingredients reached the highest.The diffusivity was controlled by film mass transfer.However,both film mass transfer and Fick's second law can be existed at the same time under different temperature and humidity.It was concluded that the diffusion of water in the traditional Chinese medicine might have been driven by a variety of diffusion mechanism,which was obviously affected by environmental factors.

This paper reviews the history of traditional Chinese medicine powder from germination, birth, prosperity to the clinical application, which is gradually reduced. And it enumerates the clinical application of traditional Chinese medicine powder taken orally, external application. The powder preparation process are outlined, summarizes the preparation results including crushing, drying, mixing, taste masking and inhibition of volatilization, sterilization with combining innovation and advice of researchers in the process of powder research. It discussed the main problems of restricting large-scale production that running through preparation, quality standard, clinical application (such as dependence of patients) of powder. Then, it forecasted that more and more hospitals and families will use traditional Chinese medicine powder to relieve pain of patients, in order to enhance the level of preparation and quality control, boosting the normalization and standardization of powder.