1. Effect of mebt/mebo on expression of mmp-2 and mmp-9 in chronic refractory wounds
Chinese Pharmacological Bulletin 2020;36(2):288-294
Aim To investigate the mechanism of MEBT/MEBO in promoting chronic wound healing by MMP-2 and MMP-9. Methods Ninety Wistar rats were randomly divided into five groups: MEBO group, rb-bFGF group, chronic wound group, acute wound group and blank group. Eighteen rats in each group were modeled as chronic refractory wounds. The ex-pression of MMP-2 and MMP-9 in wound tissues, on 3rd and 14th day, was detected by ELISA, Western blot and qRT-PCR. Results ( 1 ) Compared with chronic wound group, the time of wound healing was obviously shorter and the rate of wound healing was higher in MEBO group and rb-bFGF group ( P < 0. 05) ; meanwhile, the growth of wounds and the pathological changes of wounds were better in MEBO group and rb-bFGF group. (2) On 3rd day, the ex pression of MMP-2 and MMP-9 in MEBO group and rb-bFGF group was higher than that in chronic wound group (P <0. 05) ; however, on 14th day, the expression of MMP-2 and MMP-9 in MEBO group and rb-bF-GF group also decreased (P < 0. 05). (3) Compared with chronic wound group, the expression of MMP-2 and MMP-9 in MEBO group and rb-bFGF group increased on 3rd day (P <0. 05) , while the expression of MMP-2 and MMP-9 decreased in MEBO group and rb-bFGF group on 14th day (P <0.05). Conclusions MEBT/MEBO promoting chronic wound healing may be associated with regulating MMP-2 and MMP-9 to participate in the degradation and remodeling of ECM.
2.Study on in vitro Antioxidant Activities of Different Polar Parts of Ethanol Extract from Amomum tsao-ko
Shimei CHEN ; Biyi HUANG ; Suoyi HUANG
China Pharmacy 2020;31(8):953-956
OBJECTIVE:To evaluate in vitro antioxidant activities of 4 different polar parts of ethanol extract from Amomum tsao-ko,and to lay a foundation for the research and development of antioxidant chemical components in the plant. METHODS : The dried fruits of A. tsao-ko were crushed ,then were hearted and reflux extracted with 95% ethanol. The extraction fluid was concentrated by rotary evaporation and evaporated in water bath to obtain the ethanol extract. The extract was dispersed in water , and then extracted with petroleum ether ,ethyl acetate and n-butanol organic solvents one by one. Each solvent extract was combined and the lower water phase were collected. Finally ,the petroleum ether part ,ethyl acetate part ,n-butanol part and water part were obtained,after rotary evaporation concentration and water bath evaporation. Through in vitro antioxidant activity tests ,using 2, 6-di-tert-butyl-4-methylphenol(BHT)as positive control ,DPPH radical ,superoxide anion radical scavenging ability and Fe 3+ reducing ability of different polar parts of ethanol extract from A. tsao-ko were investigated. RESULTS :The scavenging rates of 4 polar parts of ethanol extract from A. tsao-ko on DPPH radical were all over 80%;the order of scavenging ability was ethyl acetate part>BHT>n-butanol part >petroleum ether part >water part. Those of the 4 polar parts to superoxide anion radical were between about 30%-40% mostly;the order of scavenging ability was n-butanol part >petroleum ether part >water part >ethyl acetate part > BHT;but those were weaker than their scavenging ability to DPPH r adical. The polar parts of ethanol extract also had a certain reduction ability to Fe 3+;the order of the reduction ability was n-butanol part >BHT>ethyl acetate part >petroleum ether part > water part on the whole ,but that of water part rose to the stron- gest when its concentration was 4.0 μg/mL. CONCLUSIONS: The different polar parts of ethanol extract from A. tsao-kom have certain in vitro antioxidant capacity ,but the order of antioxidant activity of different polar parts was not the same in different antioxidant activity tests ;ethyl acetate part has the 163.com strongest scave nging effect on DPPH radical ,n-butanol part has the strongest scavenging ability on superoxide anion radical and reducing ability on Fe 3+.
3.Effect of enbryonal pacreatic tissue transplantation before ranal transplantion on the treatment of type I diabetes patients complicated with ranal disorder
Yunyang WU ; Youjiang CHEN ; Mingbo WEN ; Xiangfei DING ; Huisheng ZHOU ; Huaizhou CHEN
Chinese Journal of General Surgery 2001;0(10):-
0.5ug/ml in 34 patients(79.1%).Postoperative survival rate and recover of the work ability in group T were significantly higher than those in group C.Conclusions EPTT before RT for the type I diabetes patients with renal disorder can improve the results of RT.
4.Diagnosis and treatment of aberrant thyroid cancer
Mingbo WEN ; Yunyang WU ; Youjiang CHEN ; Xiangfei DING ; Binhua TANG ; Kuiping ZHOU
Chinese Journal of General Surgery 2000;0(11):-
Objective To study the pathogenic features, diagnosis and treatment of aberrant thyroid cancer.Methods A retrospective analysis of clinical and pathological data of 29 cases of aberrant thyroid cancer was made.Results All of the 29 patients underwent operative treatment and postoperative adjuvant radiation therapy and chemotherapy. On postoperative follow up, the 5-year survival rate was 52.0%. The longest survivor patient was alive 24 years after operation.Conclusions The key to increase the survival rate of patients is early detection and timely surgical treatment. Postoperative adjuvant radiation therapy and chemotherapy are conducive to increase survival rate.
5.Detection of Chemical Warfare Agent Simulants by UV Photoionization High-Field Asymmetric Ion Mobility Spectrometry
Youjiang LIU ; Chilai CHEN ; Lehua ZHANG ; Xiaotian ZHANG ; Hongwei WANG ; Deyi KONG
Chinese Journal of Analytical Chemistry 2014;(9):1259-1263
Accurate diffusion was used to get low concentrations samples, and then the samples were detected by UV photoionization high-field asymmetric ion Mobility spectrometry ( UV-FAIMS ) . The samples were chemical warfare agent simulants ( CWAS) vapor:dimethyl methylphosphonate ( DMMP ) , dimethyl sulfoxide ( DMSO) , tributyl phosphate ( TBP ) and dimethyl sulfoxide ( DMF) . The results of FAIMS spectra data were analyzed by separation of spectra at different dispersion voltage ( DV ) and compensation voltage ( CV ) . A two-dimensional spectrum of α2 and α4 of CWAS was established. It was shown that FAIMS could identify CWAS well and have a good sensitivity. Take DMMP as a example, the detection limit was better than 0. 55 μg/L.
6.Asthenozoospermia is not correlated with 3' UTR polymorphisms of the GRP78 gene.
Hai-Mei QIN ; Rong WANG ; Xiao-Xia PANG ; Yu-Xia WEI ; Zheng-Bao LING ; Xing-Hong CHEN ; Jing-Xi WEI ; Jun-Li WANG
National Journal of Andrology 2018;24(7):596-601
ObjectiveTo investigate the correlation of the single nucleotide polymorphisms (SNPs) rs12009, rs1140763 and rs16927997 in the 3'-untranslated region (3'UTR) of the glucose-regulated protein 78 (GRP78) gene with the risk of male asthenozoospermia (AZS).
METHODSWe included 400 AZS patients in the AZS group and another 400 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs12009, rs1140763 and rs16927997 polymorphisms in the 3'UTR of the GRP78 gene in all the male subjects and analyzed the association of the three SNPs with AZS.
RESULTSThe percentage of progressively motile sperm was significantly lower in the AZS group than in the normal controls ([20.09 ± 8.18] % vs [57.16 ± 13.45] %, P <0.01). Three genotypes of CC, CT and TT and 2 alleles of C and T were found in rs12009 and rs1140763 of the GRP78 gene, and another three genotypes of GG, GA and AA and two alleles of G and A were observed in rs16927997. There were no statistically significant differences between the control and AZS groups in the frequencies of the C and T alleles in rs12009 (44.3% vs 47.3% and 55.7% vs 52.7%, P >0.05) or rs1140763 (50.0% vs 52.0% and 50.0% vs 48.0%, P >0.05) or those of the G and A alleles in rs16927997 (6.0% vs 4.4% and 94.0% vs 95.6%, P >0.05), nor in the genotypes and allele frequencies of the 3 polymorphisms (P >0.05). Furthermore, three haplotypes of C-C-A, T-C-G and T-T-A were observed in the male subjects but showed no evident correlation between the AZS and normal control groups.
CONCLUSIONSThe polymorphisms in the 3'UTR of the GRP78 gene are not correlated with the risk of male asthenozoospermia.
3' Untranslated Regions ; genetics ; Alleles ; Asthenozoospermia ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Heat-Shock Proteins ; genetics ; Humans ; Male ; Polymorphism, Single Nucleotide ; Risk
7. A feasibility study of parameter-optimized MRI as the first choice for imaging examination in patients with acute ischemic stroke
Peng CHEN ; Ruixiong LI ; Weijuan LU ; Yanling ZHONG ; Haoqiang QIN ; Qiao XIE ; Shengji WANG ; Weizhen YANG
Chinese Journal of Emergency Medicine 2019;28(9):1118-1122
Objective:
To investigate the feasibility of parameter-optimized magnetic resonance imaging (MRI) as the first choice for imaging examination in patients with acute ischemic stroke (AIS), and to assess the effects of quality improvement (QI) measures on shortening the door-to-needle time (DNT).
Methods:
A retrospective case-control study was conducted. A total of 69 AIS patients hospitalized at the Department of Neurology of the People's Hospital of Wuzhou from August 2015 to July 2018 were enrolled in the study, and the head MRI was used as the first choice for imaging examination. All patients received the intravenous thrombolytic therapy with recombinant tissue plasminogen activator (rt-PA). Patients with AIS undergoing intravenous thrombolysis from August 2015 to March 2017 were included in the control group, and those receiving intravenous thrombolysis after QI measures from April 2017 to July 2018 were included in the experimental group. QI included informing the stroke team in advance by emergency physicians, treatment process changing from serial procedure to the parallel one, optimization of MRI scanning parameters, and use of rapid test instruments. The MRI scanning time was compared between the two groups. The DNT of the two groups was compared, and paired-samples
8.Establishment and preliminary evaluation of a fluorescent recombinase-aided amplification/CRISPR-Cas12a system for rapid detection of Plasmodium falciparum
Weiyi HUANG ; Huagui WEI ; Chunfang WANG ; Junli WANG ; Liying CHEN ; Weizhong CHEN ; Yaqun LIU ; Yuzhong ZHENG ; Min LIN
Chinese Journal of Schistosomiasis Control 2023;35(1):38-43
Objective To establish a fluorescent assay for rapid detection of Plasmodium falciparum based on recombinaseaided amplification (RAA) and CRISPR-Cas12a system,and to preliminarily evaluate the diagnostic efficiency of this system.. Methods The 18S ribosomal RNA (rRNA) gene of P. falciparum was selected as the target sequence, and three pairs of RAA primers and CRISPR-derived RNA (crRNA) were designed and synthesized. The optimal combination of RAA primers and crRNA was screened and the reaction conditions of the system were optimized to create a fluorescent RAA/CRISPR-Cas12a system. The plasmid containing 18S rRNA gene of the P. falciparum strain 3D7 was generated, and diluted into concentrations of 1 000, 100, 10, 1 copy/μL for the fluorescent RAA/CRISPR-Cas12a assay, and its sensitivity was evaluated. The genomic DNA from P. vivax, P. malariae, P. ovum, hepatitis B virus, human immunodeficiency virus and Treponema pallidum was employed as templates for the fluorescent RAA/CRISPR-Cas12a assay, and its specificity was evaluated. Fifty malaria clinical samples were subjected to the fluorescent RAA/CRISPR-Cas12a assay and nested PCR assay, and the consistency between two assays was compared. In addition, P. falciparum strain 3D7 was cultured in vitro. Then, the culture was diluted into blood samples with parasite densities of 1 000, 500, 200, 50, 10 parasites/μL with healthy volunteers’ O-positive red blood cells for the RAA/CRISPR-Cas12a assay, and the detection efficiency was tested. Results The Pf-F3/Pf-R3/crRNA2 combination, 2.5 μL as the addition amount of B buffer, 40 min as the RAA reaction time, 37 °C as the reaction temperature of the CRISPR-Cas12a system were employed to establish the fluorescent RAA/CRISPR-Cas12a system. Such a system was effective to detect the plasmid containing 18S rRNA gene of the P. falciparum strain 3D7 at a concentration of 1 copy/μL, and presented fluorescent signals for detection of P. falciparum, but failed to detect P. ovum, P. malariae, P. vivax, T. pallidum, hepatitis B virus or human immunodeficiency virus. The fluorescent RAA/CRISPR-Cas12a system and nested PCR assay showed completely consistent results for detection of 50 malaria clinical samples (kappa = 1.0, P < 0.001). Following 6-day in vitro culture of the P. falciparum strain 3D7, 10 mL cultures were generated and the fluorescent RAA/CRISPR-Cas12a system showed the minimal detection limit of 50 parasites/μL. Conclusion The fluorescent RAA/CRISPR-Cas12a system is rapid, sensitive and specific for detection of P. falciparum, which shows promising value for rapid detection and risk monitoring of P. falciparum.
9.Association of SLC6A4 gene c.*670T>G polymorphism with the risk for asthma and peripheral blood cytological characteristics among ethnic Zhuang Chinese population.
Gao CHEN ; Jianguo XU ; Shuai WEI ; Minlv MENG ; Chen LAN ; Chunru ZHAO ; Yingjiao MA
Chinese Journal of Medical Genetics 2023;40(10):1228-1235
OBJECTIVE:
To assess the association of SLC6A4 gene c.*670T>G polymorphism with the risk for asthma and peripheral blood cytological characteristics among ethnic Zhuang Chinese from Guangxi, China.
METHODS:
From May 2017 to March 2020, 258 patients diagnosed with asthma and 244 healthy controls were recruited from the Affiliated Hospital of Youjiang Minzhu Medical College and the People's Hospital of Hechi. Genotypes of the c.*670T>G polymorphism were determined by Sanger sequencing. Flow cytometry was used in combination with an electrical impedance method for the counting and classification of peripheral blood cells.
RESULTS:
Compared with the T allele, the G allele of the c.*670T>G polymorphism was associated with the risk for asthma in the population (OR = 1.54, 95%CI = 1.15-2.06; P = 0.004). Compared with the GT and TT genotypes, homozygous GG genotype also comprised a risk factor (OR = 1.66, 95%CI = 1.16-2.38; P = 0.005). Stratification of the risk factors showed that the homozygous GG genotype has increased the risk of asthma in males and urban residents (P < 0.01). The erythrocyte, hemoglobin and platelet counts of the asthma group were significantly higher than the control group (P < 0.001). The GG, GT and TT genotypes have respectively accounted for 82.35%, 17.65% and 0% of the samples with platelets exceeding the normal value. The overall platelet level of GG genotype was higher than GT+TT genotype (P < 0.05). The significant association was verified by the false positive report probability, and at a prior probability level of 0.1, G vs. T false positive probability was 0.071, and GG vs. GT+TT false positive probability was 0.153.
CONCLUSION
The GG genotype of the c.*670T>G polymorphism is associated with the risk for asthma among ethnic Zhuang Chinese from northwest Guangxi. Above finding has also enriched the genotypic data and peripheral blood phenotype for this polymorphism.
Male
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Humans
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East Asian People
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China
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Genotype
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Alleles
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Asthma/genetics*
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Serotonin Plasma Membrane Transport Proteins
10.Association of SNP of leukocyte differentiation antigen-CD40 gene and its serum level with ischemic stroke.
Jianming CHEN ; Huatuo HUANG ; Qiuran YUAN ; Hongcheng LUO ; Yang XIANG ; Chunfang WANG ; Lanqing MENG ; Yesheng WEI
Chinese Journal of Preventive Medicine 2016;50(3):239-243
OBJECTIVETo investigate the association of SNP of CD40 gene and its serum levels with ischemic stroke (IS).
METHODSA total of 202 IS patients from a hospital of Baise city were enrolled in case group from May 2013 to November 2014. At the same time, 109 healthy people who had physical check-ups in the outpatient department at the same hospital were enrolled in the control group. All participants were from Guangxi Zhuang Autonomous Region and unrelated to each other. 3 ml venous blood were collected on the premise of informed consent. The single nucleotide polymorphisms of CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A were analyzed using a Snapshot SNP genotyping assays, and the serum levels of CD40 were tested by ELISA. t-test was used to compare the serum levels of CD40 between the case and control group, and the genotypes at different locuses in case group; χ(2) test was used to compare the distribution differences of the CD40 gene locuses in different genotypes and allele between the case group and the control group; alleles was established as independent variables, the occurrence of the IS as dependent variable, and expressed relative risk with OR (95%CI) value.
RESULTSIn the case group, the frequency of CC, CT and TT genotypes in CD40 gene rs1883832 C/T were 21.78% (44/202), 49.51% (100/202) and 28.71% (58/202), respectively, and 33.17% (66/199), 48.74% (97/199), 18.09% (36/199) in the control group, respectively, the differences between the two groups was significant (χ(2)=9.57, P=0.008). The CD40 serum levels were (62.7 ± 24.5) pg/ml in the case group, which was higher than that in the control group (45.3 ± 17.2) pg/ml (t=8.97, P<0.001). The serum levels of TT and CT genotypes in CD40 gene were (65.9 ± 26.3) and (64.3 ± 25.9) pg/ml, respectively, and the differences were significant when comparing with CC genotype (t equaled 5.34 and 5.03, respectively, P<0.001). The risk of developing IS was 1.56 times higher in 1883832 T allele carriers than that in rs1883832 C allele carriers (OR=1.56, 95% CI: 1.18-2.06); Combined genotype analysis displayed that CD40 gene rs1883832 C/T, rs13040307 C/T, rs752118 C/T and rs3765459 G/A polymorphisms showed strong linkage disequilibrium, the case group TCCA haplotype was tested to be associated with a significantly increased risk of IS as compared with that in the control group(OR=2.49; 95%CI: 1.13-5.48).
CONCLUSIONCD40 gene rs1883832 C/T polymorphism and its TCCA haplotype were possibly associated with ischemic stroke, and the susceptibility gene for ischemic stroke may be rs1883832 T allele.
Alleles ; CD40 Antigens ; blood ; genetics ; Case-Control Studies ; Cell Differentiation ; China ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Humans ; Polymorphism, Single Nucleotide ; Stroke ; blood ; genetics