Purpose:Our study is to find out the inhibitory action of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) on ovarian cancer cell line cultured in vitro. Methods:MTT was applied to assay the inhibiting action of various concentration of TRAIL on two ovarian cancer cell lines of 3AO and HO-8910.The apoptosis rates were measured by flow cytometry. Results:The growth of human ovarian cancer cells was effectively inhibited by TRAIL. A clear dose- and time-dependent correlation between TRAIL concentration and the degree of apoptosis induction was observed with up to 43.20% apoptotic cells after 24 h of incubation with 50 ng/ml TRAIL. The cells assumed typical cell apoptosis configuration. Conclusions:TRAIL can effectively inhibit the growth of ovarian cancer cells and induce apoptosis of the cells.

Epithelial ovarian cancer, which is one of the most lethal gynecological tumors, is also the biggest dififculty in the diagnosis and treatment of obstetrics and gynecology. In addition to the lack of early clinical symptoms and effective diagnostic methods, the reason includes the lack of accurate and comprehensive understanding of the development of epithelial ovarian cancer. Thus a lot of research aimed at better grasp of its pathogenesis, which is expected in the future progress of its diagnosis and treatment. Currently, three pathogenesis of epithelial ovarian cancer widely accepted are high gonadotropin theory, “dualism” hypothesis and stem cell hypothesis. This study discussed the theory above.

Objective To investigate the effects of sulindac metabolites on the proliferation and apoptosis of human umbilical vein endothelial cell line ECV304 in vitro Methods The proliferation of ECV304 was determined by methyl thiazolyl tetrazolium (MTT) method The cell cycle, apoptosis and the ultrastructure of ECV304 were detected by flow cytometry (FCM) and electron microscopy respectively Results MTT assay showed that sulfide inhibited the proliferation of ECV304, and the effects was dose dependent, the 50% inhibiting concentration (IC 50 ) was 200 ?mol/L FCM showed that sulfide changed cell cycle distribution, the cell cycle were: Go G 1 phase [control group (77 7?1 6)%, sulfone group (75 6?2 1)%, sulfide group (46 1?1 6)%] S phase [control group (13 6?1 2)%, sulfone group (16 4?2 3)%, sulfide group (27 3?2 1)%], G 2 M phase [control group (8 6?0 7)%, sulfone group (8 0?0 5)%, sulfide group (26 6?3 5)% ] The apoptosis rates in control group, sulfone group and sulfide group were (6 1?3 4)%, (4 8?2 1)% and (51 9?5 7)%, respectively Compared with the control group, sulfide can reduce the proportion of G 1 phase, increase the proportion of S phase and G 2 M phase significantly ( P

Objective To investigate the inhibitory effect of the mammalian target of rapamycin (mTOR) inhibitor, sirolimus on expression of hypoxia-inducible factor (HIF)1? protein and growth of ovarian carcinoma in an athymic mouse xenogeneic transplant model of ovarian cancer. Methods Four groups of female nude mice were inoculated subcutaneously with SKOV3 cells. After inoculation, mice were treated with saline, rapamycin alone, paclitaxel alone and sirolimus+paclitaxel. In each tumor protein expressions of HIF-1?,bcl-2 and apoptosis were determined by immunohistochemistry and RT-PCR. Results In sirolimus and sirolimus +paclitaxel groups protein expression of HIF-1? was inhibited. Tumor burden in rapamycin alone, sirolimus +paclitaxel, and paclitaxel alone was reduced by 47.9%( P 0.05) respectively compared with controls. Cell apoptosis inder in sirolimus alone(36), sirolimus +paclitaxel(40), paclitaxel alone(22),increased compared with control(15), while expression of bcl-2 decreased compared with control. Conclusion Sirolimus inhibited protein expression of HIF-1?, increased tumor apoptosis and decreased tumor growth.

Objective To observe whether endothelial cell (EC) progenitors (CD~+_(34)-positive mononuclear cells) participated in neovasculogenesis of ovarian epithelial carcinoma through in vitro and in vivo experiments, and to explore the mechanism of tumor neovasculogenesis. Methods CD~+_(34)-positive mononuclear cells were isolated from peripheral blood of ovarian epithelial carcinoma patients by means of magnetic beads coated with antibody to CD~+_(34), plated on culture dishes coated with human fibronectin in endothelium medium, and examined by using RT-PCR, fluorescence-activated cell sorting (FACS) and nitric oxide (NO) assay kit for the expression of EC lineage-markers. EC-like cells were labeled with DiI ex vivo, and injected into immunodeficiency mice model with transplanted hypodermic SKOV3 by caudal vein. After 4-6 weeks, the tumor was resected and examined by confocal microscopy, and immunohistochemistry. Results In vitro, CD~+_(34)-positive mononuclear cells differentiated into ECs. In animal models of SKOV3, EC progenitors (CD~+_(34)-positive mononuclear cells) incorporated into sites of neovasculogenesis in tumor, 4-6 weeks later DiI-labeled cells incorporated into capillaries and small arteries. Conclusions The neovasculogenesis in human ovarian epithelial carcinoma involves angiogenesis and vasculogenesis.

Objective To investigate the correlation of hypoxia-inducible factor (HIF)-1? and va scular endothelial growth factor (VEGF) or micro-vessel density ( MVD). Methods The ovarian cancer cell line SKOV3 was transplanted into nude mice to form xenog eneic tumor. Mice were treated with rapamycin 4mg/kg,sulindac 100 mg/(kg.d) a nd saline 200 ?l respectively. Expression of HIF-1? and VEGF proteins and MV D were determined by immunohistochemistry. The mRNA of Glut1 and VEGF was studi ed by RT-PCR. Results The positive expression of HIF-1? and VEGF was moderate to strong, and MVD was high (31?8) in control group. In rapamycin treated group, the expression of H IF-1? was inhibited to weak positive or negative (P

Objective To investigate the inhibitory effects of Snail—— a zinc finger transcription factor, anti-sense plasmid on the invasion of ovary carcinoma cell lines in vitro. Methods Four human ovarian carcinoma cell lines ES-2,OVCAR3,HO8910,HO8910PM were analyzed for the expression of Snail and E-cadherin mRNA by RT-PCR.Then anti-Snail plasmid transfection was introduced into HO8910 cells using lipofectin 2000 reagent to investigate the inverse correlation between E-cadherin and Snail expression, and the inhibitory effects of anti-sense Snail were also detected by Transwell motility assay and Matrigel invasion assay. Results The results demonstrated the presence of Snail mRNA in ES-2,HO8910,HO8910PM detected by RT-PCR. By contrast, E-cadherin mRNA was only detected in OVCAR3 by RT-PCR.The inverse relationships were further observed by transient transfection of anti-sense Snail into HO8910 cells, which showed the down-regulated expression of Snail and the re-expression of E-cadherin. The Snail mRNA levels were 0.897?0.005,0.865?0.010,0.338?0.014 after 0,24,48 h of transfection and that of E-cadherin were 0,0.130?0.001,0.217?0.005 respectively, the difference was significant between different time points (P

With the emergence of new concepts, methods and techniques in modern medicine, the basic research and clinical treatment for gynecological oncology has rapidly developed in the past couple of years. Evidence-based medicine, individualized therapy and microinvasive therapy give us more opportunities to treat the patient. Nowadays, it is possible for the clinic to implement new approaches to improve the outcome and the quality of life for the patients with minimal side effects at the same time. There has been a lot of reports from in vitro studies suggesting that gonadotrophin may play an important role in tumorigenesis, estrogen may promote tumor cell proliferation through PAX2 gene. Survivin, a protein serving as an apoptosis inhibitor, may be crucial for the regulation of tumor cell proliferation and apoptosis.

Objective To investigate the chemosensitivity of ovarian cancer SKOV3ip1 multicellular aggregates to cisplatin and taxol and to explore the possible mechanisms accounting for the effect Methods Liquid overlay system was employed to obtain multicellular aggregates (MCA) We detected the resistance with trypan blue exclusion testing, clonogenic assay, cell cycle profiles and apoptosis with flow cytometry Results MCA cells showed higher cell viability than monolayer cells ( P =0 045 and P =0 003, respectively). After 40 ?mol/L cisplatin exposure for 12 hours, no clone (≥50 cells) was formed After 10 ?mol/L taxol exposure for 12 hours, the clone formation showed significant difference in 100 cell group between multicellular aggregates and monolayer cells ( P

Objective To assess the efficacy of ultrasound contrast agent Levovist in evaluating vascularization of ovarian tumors.Methods Nineteen ovarian lesions (seven benign ovarian tumors,twelve malignant ovarian tumors) were submitted to color Doppler flow imaging (CDFI) before and after i.v.Levovist for examining their vascularization,including the number of blood vessel,vessel torsion and Doppler signal enhancement. And then receiver operator characteristic(ROC) curves were established.Results Compared to benign tumors',after contrast color Doppler signals in malignant tumors enhanced obviously and character characteristic vessel morphologies were observed.Vessel numbers and tortuosity increased obviously.Doppler signal enhancement appeared earlier,arrival to peak enhancement was quicker,and duration longer.ROC showed time to commencement for the enhancement ≤50 s,time to peak ≤100 s and enhancement duration ≥400 s,at which the sensitivity and specificity were the highest.Conclusions Levovist,as a contrast agent, increases the intensity of color Doppler signals obviously,and allows a more complete display of the vascular patterns of ovarian tumor.It improves markedly the role of CDFI in the diagnosis and differentiation of ovarian tumors.