1.A Analysis of 273 Strains Clinical Isolated Enterococci
Youlin LI ; Quansheng LI ; Yougao WU ; Qiuxiang HU ; Kaisen CHEN
Chinese Journal of Nosocomiology 2009;0(22):-
OBJECTIVE To explore the drug-resistant situation and patients′ basis condition of clinical isolated Enterococcus from Jan to Dec in 2008 to offer evidence for drug-resistant monitoring and clinical antibiotics usage.METHODS All clinical specimens were isolated and cultured and identification conform to Standard′ Operation.The bacteria were identified by using the automatic microorganism analyzer VITEK-2,and bacteria′s drug susceptibility tests were performed using counterparts panel.RESULTS 273 strains Enterococci were isolated,of which E.faecalis were 158 strains,E.faecium were 109 strains,the others were 6 strains.The isolated HLAR E.faecalis were 80 strains and HLAR E.faecium were 10 strains,the isolated.ratio of HLAR was different(P
2.Analysis of Polarizing Microscopic Characteristics and X-ray Diffraction Fingerprint of Mineral Medicine Maifanitum
Sicheng WU ; Yulu MA ; Wenguo YANG ; Fang FANG ; Ying WANG ; Wei YANG ; Shilin DAI ; Baofei YAN ; Jin ZHAO ; Xiuxiu WANG ; Qian ZHAO ; Xiaohua BAO ; Jianping ZHANG ; Chenyu XU ; Shengjin LIU
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(13):166-172
ObjectiveTo analyze the polarized light microscopic characteristics, the composition of physical phases and their relative contents of Maifanitum from different origins, and to establish the Fourier characteristic fingerprint of Maifanitum powder crystals by X-ray diffraction(XRD). MethodA total of 26 batches of Maifanitum samples were selected, and the microscopic characteristics of the sample powders and grinding flakes were observed by polarized light microscopy under single polarized light and orthogonal polarized light, and the main phase compositions and their relative contents were analyzed by powder crystal XRD technique, and the XRD Fourier characteristic fingerprint of Maifanitum was established. The incident light source of XRD was Cu target Kβ radiation, the light tube voltage and light tube current were 40 kV and 40 mA, respectively, the divergence slit was 1°, the scattering slit was 1°, the receiving slit was 0.2 mm, the scanning speed was 5°·min-1 with continuous scanning and scanning range of 5-90°(2θ), and the step length was 0.02°. ResultThe polarized light micrographs of powders and grinding flakes of Maifanitum were obtained, and the main phases were plagioclase, potassium feldspar and quartz, and a few samples also contained illite, pyrite, iron dolomite, calcite, iron amphibole and chlorite, etc. The relative total content of feldspar phases was 61.9%-82.4%, and the relative content of quartz was 12.6%-33.6%. The XRD Fourier fingerprint analysis method of Maifanitum with 13 common peaks as the characteristic fingerprint information was established, and the similarity calculated by the mean correlation coefficient method was 0.920 9-0.997 7, the similarity calculated by the mean angle cosine method was 0.940 5-0.998 4, the similarity calculated by the median correlation coefficient method was 0.921 1-0.997 5, and the similarity calculated by the median angle cosine method was 0.947 5-0.998 2. ConclusionThe polarized light microscopic identification characteristics of Maifanitum are mainly plagioclase, quartz and potassium feldspar, and the technique of powder crystal XRD Fourier fingerprint analysis can be used for the identification of Maifanitum.