Objective To investigate T cell-mediated anti-HBV effects of dendritic cells (DCs) sensitized by HBsAg in patients with HBV infection in vitro. Methods DCs isolated from peripheral blood mononuclear cells of HBV patients were cultured and proliferated in vitro, and then were stimulated with pure HBsAg before DCs maturation, and co-cultured with auto-T lymphocytes after DCs maturation. The T lymphocytes were then harvested 5 days later and added into the supernatant of 2.2.15 cell cultures. The supernatants were collected on 1st, 3rd, 5th and 7th day, respectively. ELISA was employed to detect HBeAg and the secretion of HBsAg, as well as to measure the concentration of IFN-? and IL-12 in culture supernatents. Results The proliferation and cytotoxicity of auto-T cells were markedly enhanced in the antigen stimulated DCs group compared with that of DCs without loaded antigen and only T lymphocyte group (P

Ojective To evaluate the effect of implatation of prosthesis under the periosteum via oral incision on mentoplasty. Methods The buccal morphology and structure of cadaver was studied with topographic methods. 43 cases of mentoplasty was treated by implatation of prosthesis under the periosteum via oral incision. Results The mandibular shape of all cases treated by mentoplasty were improved with successful and satisfactory effects. No complication occurred. Conclusions It suggested that the implatation of prosthesis under the periosteum via oral incision was a safe, effective procedure for mentoplasty.

Objective To study arterial distribution of rectus abdominis musculocutaneous flap and to evaluate whether it can be divided into several units for reconstruction. Methods The arteries of the rectus abdominis musculocutaneous flap were studied on 60 sides of cadavers by dissection and angiography. Results The superior epigastric artery (SEA) and the inferior epigastric artery (IEA) continued in a longitudinal direction. Most of their branches took on a typical spiral configuration and communicated with each other within muscle above the level of umbilicus. Many perforating arteries penetrated through the anterior rectus sheath to get to the overlying skin, but the highest concentration of major perforators were in the paraumbilical area. The inferior epigastric artery was more significant than superior epigastric artery in supplying the skin of the musculocutaneous flap. Based on thefstudies of the vascular anatomy of muscles, we could classify arterial distribution into 3 types: type Ⅰ (SEA 26.5 %, IEA 34.6%) revealed a single main intramuscular artery: type Ⅱ (SEA 64.7 %, IEA 48.1%) had two major intramuscular branches; type Ⅲ (SEA 8.8%, IEA 17.3 %) revealed three intramuscular branches. Our anatomic studies showed that the superior and inferior epigastric artery bifurcated or divided into more than two main branches in the majority of cases (SEA 73.5%, IEA 65.4%). Conclusion The rectus abdominis musculocutaneous flap could often be divided into several regions for breast construction which is based on the distribution of each branch of the artery.

Objective To evaluate the difference of PC values based on GBZ 97-2002 and on GBZ 97-2009 for lung cancer cases in Chinese uranium miners.Methods Using 19 lung cancer data ascertained in the past epidemiological study,PC values were calculated according to GBZ 97-2002 and GBZ 97-2009.Results In the 19 lung cancer cases,those cases that could not be judged as radiogenic cancers based on GBZ 97-2002,but may be judged as radiogenic cancers with GBZ 97-2009.The probability was enlarged by 1.1 times at least.The major reason was that the used value was the upper limit of 95％ in GBZ 2009 but the median in 2002.Conclusions Compared to GBZ 97-2002,the criteria value of PC in GBZ 97-2009 drops obviously.

The dermal sheath surrounding the outside of the hair follicle maintains and regenerates the dermal papilla, a necessary component for hair regeneration. Dermal sheath cells participate in skin wound healing and have some characteristics of adult stem cells such as immuneprivilege, multiple differentiation and plasticity. It is likely that dermal sheath cells will be the new cynosure in the research on wound healing and tissue engineering. Here we review the studies of hair follicle dermal sheath in present years.
Dermis ; cytology ; physiology ; Hair Follicle ; cytology ; physiology ; Humans ; Stem Cells ; physiology ; Tissue Engineering ; methods ; Wound Healing ; physiology

Objective:To summarize the effect of Ilizarov technique in the treatment of limb contracture secondary to venous malformation.Methods:From January 2015 to June 2019, nine cases of limb contracture secondary to venous malformation, from Peking University Third Hospital and Rehabilitation Hospital of the National Research Center for Rehabilitation Technical Aids, were treated with Ilizarov technique, including four males and five females, aged 9 to 35 years, with an average age of 19.5 years. There were two cases of flexion of the hip and knee, four cases of flexion of the knee, two cases of flexion of the knee with talipes equinovarus, and one case of flexion of the wrist. Ilizarov technique was used for treatment after the evaluation of the multi-disciplinary treatment team. According to the safe zone of wire fixation and the limbs deformities character, the preassembled external fixator was connected to the relevant deformed limbs, and the tendons were released at the same time. Limbs deformities were corrected gradually by adjusting the external fixator. For patients with severe pain or rapid development of the affected area, it is appropriate to perform ultrasound-guided injection of lauromacrogol and physical therapy to promote the function of joints.Results:During the treatment, there were no neurovascular injury, peripheral blood circulation disorder, infection event of injection channel and incision, or deep vein thrombosis. The follow-up were 6 to 40 months, with an average of 30.5 months. All the contractures of the hip and knee joints, most of the deformities of the wrist and ankle joints were corrected and the pain disappeared.Conclusions:Ilizarov technique is a minimally invasive, safe and effective method for the treatment of limb contracture secondary to venous malformation.

Objective:To summarize the effect of Ilizarov technique in the treatment of limb contracture secondary to venous malformation.Methods:From January 2015 to June 2019, nine cases of limb contracture secondary to venous malformation, from Peking University Third Hospital and Rehabilitation Hospital of the National Research Center for Rehabilitation Technical Aids, were treated with Ilizarov technique, including four males and five females, aged 9 to 35 years, with an average age of 19.5 years. There were two cases of flexion of the hip and knee, four cases of flexion of the knee, two cases of flexion of the knee with talipes equinovarus, and one case of flexion of the wrist. Ilizarov technique was used for treatment after the evaluation of the multi-disciplinary treatment team. According to the safe zone of wire fixation and the limbs deformities character, the preassembled external fixator was connected to the relevant deformed limbs, and the tendons were released at the same time. Limbs deformities were corrected gradually by adjusting the external fixator. For patients with severe pain or rapid development of the affected area, it is appropriate to perform ultrasound-guided injection of lauromacrogol and physical therapy to promote the function of joints.Results:During the treatment, there were no neurovascular injury, peripheral blood circulation disorder, infection event of injection channel and incision, or deep vein thrombosis. The follow-up were 6 to 40 months, with an average of 30.5 months. All the contractures of the hip and knee joints, most of the deformities of the wrist and ankle joints were corrected and the pain disappeared.Conclusions:Ilizarov technique is a minimally invasive, safe and effective method for the treatment of limb contracture secondary to venous malformation.

Objective To explore the influence factors for femoral head necrosis after treatment of femoral neck fractures with dynamic hip screw (DHS) and anti-rotation screw.Methods A retrospective case series analysis was made on the clinical data of 106 cases of femoral neck fractures who had undergone fixation with DHS and anti-rotation screw between May 2010 and May 2015.There were 59 males and 47 females,with an average age of 57.0 years (range,27-76 years).By Garden classification,there were 27 cases of type Ⅱ,51 cases of type Ⅲ and 28 cases of type Ⅳ.All cases were divided into femoral head necrosis group (18 cases) and none-necrosis group (88 cases) according to the radiographs of the fractured hip at the follow-up.Univariate analyses and a multivariate logistic regression analysis were made to test whether the following factors were significantly associated with femoral neck necrosis:sex,age,Garden classification,Pauwels classification,Singh index,injury-to-surgery time interval,reduction methods,reduction quality,complete weight-bearing time,implant removal and the time cost of implant removal surgery.Results All the 106 patients obtained a mean follow-up of 49 months (range,26-76 months).Femoral head necrosis occurred in 18 cases (17.0％).In univariate analyses,Garden classification,reduction quality,implant removal and long time of the implant removal surgery were significantly associated with femoral head necrosis (P ＜ 0.01).In multivariate logistic regression analysis,high level of Garden classification(95％ CI 0.008,0.998,P ＜ 0.05),implant removal and long time of the implant removal surgery (95％ CI 0.000,0.143,P ＜ 0.01) were found to have a significant effect on femoral head necrosis development.Conclusions Fracture displacement,removal of internal fixation and broadening the screw canal,which cand hinder the blood supply of femoral head,will enhance the rate of femoral head necrosis.As a result,it needs prudent consideration to remove internal fixation after internal fixation with DHS combined with anti-rotation screw for femoral neck fractures.When it is difficult to remove the anti-rotation screw,it is better to give up,rather than to force a removal.