Parsitological surveys were conducted to study the recent trend of hookworm infection in remote rural areas of Miyazaki Prefecture, south Japan. Kate's thich smear and test-tube filter paper culture techniques were performed on 837 fecal samples collected from four villages. Prevalence rate of hookworm infection was 13.0% average and that of other intestinal helminths was less than 2.0%. In these districts Ancylostoma duodenale was predominant and species ratio with Necator americanus was 81: 27. Mixed infecttion was few as compared with past record. Prevalence rate and age distribution of N. americanus infection showed a considerable difference between sexes and especially the infection rate was higher in women than man, also the carriers ratio with man was 8: 1. As to the geographical distribution of both species in a village there was a certain tendency of single species infection in a unit of small community.
However, prevalence of hookworm infection in these communities showed a considerable distinction with one another.