Objective To study the antigen presenting function of keratinocytes to CD8 + T cells with specific TCR.Methods PAM212transfectants,which express H-2K b /ovalbumin complex,aa257-264,were used as stimulating cells,and naive or primed CD8 + T cells with ovalbumin specific TCR were used as respon-ders.3 H-thymidin uptake values were used as indicators of T cell proliferation.Results With prestimulation of IFN-?,the proliferation of primed T cells but not naive T cells could be stimulated by PAM-OK cells.Conclusion Keratinocytes have the potential to stimulate the proliferation of TCR specific CD8 + T cells,which might explain the persistence of local immune reaction in some involved skin.
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