Object To study the chemical constituents of Bidens bipinnata L Methods Isolation and purification were carried out on silica gel, ODS column, and Sephadex LH 20, HPLC, identified by physicochemical properties and structurally elucidated by spectral analysis Results From n BuOH extract of B bipinnata, 12 compounds were obtained and identified as: 6 O ? D glucopyranosyl 6, 7, 3′, 4′ tetrahydroxyaurone (Ⅰ), 6 O (6″ acetyl ? D glucopyranosyl) 6, 7, 3′, 4′ tetrahydroxyaurone (Ⅱ), quercetin 3 O ? D glucopyranoside (Ⅲ), quercetin 3 O ? L rhamnoside (Ⅳ), iso okanin 7 O ? D glucopyranoside (Ⅴ), esculin (Ⅵ), (E) 2 hexenyl O ? D glucopyranoside (Ⅶ), n hexyl O ? D glucopyranoside (Ⅷ), isopentyl O ? D glucopyranoside (Ⅸ), n butyl O ? D fructofuranoside (Ⅹ), n butyl O ? D fructofuranoside (Ⅺ), n butyl O ? D fructopyranoside ( ⅩⅡ ) Conclusion All of these compounds, except Ⅰ and Ⅴ, are obtained from the plant for the first time

Object To study the chemical constituents in Bidens bipinnata L Methods Isolation and purifica tion were carried out on silica gel, ODS column and HPLC; the chemical constitue nts were identified by physico-chemical properties and structurally elucidated by spectral analysis Results From EtOAc extract of B bipinnata, eight compounds were obtained and identified as: salicyl i c acid (Ⅰ), 9, 12, 13-trihydroxy-10, 15-octadecadienoic acid (Ⅱ), 9, 12, 13-trihydroxy-10-octadecaenoic acid (Ⅲ), benzyl O-?-D-glucopyranoside (Ⅳ), benzen ethyl O-?-D-glucopyranoside (Ⅴ), (Z)-3-hexenyl O-?-D-glucopyranoside (Ⅵ), eugenyl O-? -D-glucopyranoside (Ⅶ), 3-methyl-2-(2-pentenyl)-4-O-?-D-glucopyranosyl-△ 2-cyclopenten-1-one (Ⅷ) Conclusion All of these compounds, with the exception of Ⅰ, are obtained from the plant for the first time

Upper ministernotomy is frequently selected in aortic valve replacement by minimary invasive cardiac surgery. However, retrograde cardioplegia cannulae cannot be inserted to some sites. CT examinations in our department revealed that lower ministernotomy can be used for surgery of the aortic valve in many Japanese cases. The usefulness of 2 approaches was examined in 68 cases with aortic valve disease who received aortic valve replacement by minimary invasive cardiac surgery from January 1997 to March 2002: Those who received upper ministernotomy (U group) and those who received lower ministernotomy (L group). Retrograde cardioplegia is frequently used in aortic valve replacement for myocardial protection. Those in the L group showed effectiveness in myocardial protection and in securing the operation field except in cases who were switched to full sternotomy. In the L group, the MAZE operation was performed and no significant differences were observed in aortic cross-clamping time, artificial cardiorespiratory time, operation time, bleeding amount and other factors. Lower ministernotomy was more effective than upper ministernotomy in myocardial protection by retrograde cardioplegia and securing the operation field in aortic valve replacement by minimally invasive cardiac surgery.

A conventional reoperation via full sternotomy approach is associated with a higher risk of heart injury compared with first time operations. We employ a minimally invasive cardiac surgery (MICS) for valve reoperations in order to minimize dissection of sternal adhesions. We evaluated MICS for mitral reoperation in this report. We retrospectively analyzed 20 patients (group P) who underwent mitral reoperation via partial lower hemisternotomy (PLH) from July 1997 through March 2002, and 13 patients (group F) who underwent mitral reoperation via full sternotomy from April 1990 through June 1997. All patients received mitral valve replacement in both groups. Concomitant Maze procedures were significantly more frequent in group P (group P: n=8, group F: n=1). Aortic cross clamp times were significantly longer in group P (group P: 110±5min, group F:87±11min). The blood loss during operations was significantly less in group P (group P: 666±100ml, group F: 2, 405±947ml). Postoperative ventilation time and the length of intensive care unit stay were significantly shorter in group P. In group P and F the occurrence of a heart injury associated with sternotomy was 0/20 (0%), 2/13 (15%) respectively. Hospital mortality was 0/20 (0%), 2/13 (15%) respectively. There were neither any hospital deaths nor any postoperative major complications in group P. We conclude that PLH for mitral reoperations could be performed safely and is an alternative approach for mitral reoperations.

Oral administration of red ginseng extracts (1% in diet for 40 weeks) resulted in the significant suppression of spontaneous liver tumor formation in C3H/He male mice. Average number of tumors per mouse in control group was 1.06, while that in red ginseng extracts-treated group was 0.33 (p<0.05). Incidence of liver tumor development was also lower in red ginseng extracts-treated group, although the difference from control group was not statistically significant. Anti-carcinogenic activity of white ginseng extracts, besides red ginseng extracts, was also investigated. In the present study, the administration of white ginseng extracts was proven to suppress tumor promoter-induced phenomena in vitro and in vivo. It is of interest that oral administration of the extracts of Ren-Shen-Yang- Rong-Tang, a white ginseng-containing Chinese medicinal prescription, resulted in the suppression of skin tumor promotion by 12-o-tetradecanoylphorbol-13-acetate in 7,12-dimethylbenz[a]anthracene-initiated CD-1 mice. These results suggest the usefulness of ginseng in the field of cancer prevention.
Animal ; Anticarcinogenic Agents/*pharmacology ; Female ; Liver Neoplasms, Experimental/*prevention & control ; Male ; Mice ; Mice, Inbred C3H ; *Panax ; Plant Extracts/pharmacology ; Plant Roots ; Skin Neoplasms/*prevention & control