AIM: In order to understand the role of chlamydia pneumoniae in the course of macrophages transformation into foam cells experiments with chlamydia pneumoniae standard strain AR-39 wese made. METHOD: C57 BL/6J Mouse peritoneal macrophages C57 BL/6J wese incubated 24 h, and they were divided into 6 groups to be incubated continually: A 1～DMEM; A 2～DMEM+10 6 IFUs/L AR-39; B 1～DMEM+10 mg/L LDL; B 2～DMEM+10 mg/L LDL+10 6 IFUs/L AR-39; C 1～DMEM+10 mg/L OxLDL ;C 2～DMEM+10 mg/L OxLDL+10 6 IFUs/L AR-39. 72 h later, morphological changes of the cells were observed and of intracellular cholesterol of content was detected. RESULTS: 1、Morphological studies: there were no lipid particles in A 1,A 2 and B 1 groups, but the lipid particles could be found in B 2、C 1 and C 2 group, Among six groups, the most lipid particles were seen in C 2 groups. 2、Biochemical detection:The ratio of cholesterol ester to total cholesterol was much higher than 50% in B 2、C 1 and C 2 groups, but was less than 50% in A 1、A 2 and B 1 groups. CONCLUSION: Chlamydia pneumoniae may have played a role in promoting C57 BL/6J mouse peritoneal macrophages into foam cells.

AIM: To study the relationship between Chlamydia pneumoniae (Cpn) and spontaneous brain bleeding. METHODS: The IgG and IgM antibody titers to Cpn in peripheral blood from 9 patients of spontaneous brain bleeding were detected by microimmunofluorescence antibody technique (MIF). The Cpn DNA of arteries in brain bleeding region from these cases was detected by polymerase chain reaction (PCR). The Cpn major outer membrane protein (MOMP) expression of arteries in brain bleeding region from these cases were tested by Western blot. RESULTS: All the geometric average titer to IgG antibody (190 3?3 2) and the positive rate of IgG antibody (85 2%) and IgM antibody (30 8%) were significantly higher in spontaneous brain bleeding patients than those (48 1?2 0, 48 1% and 4 8%) in control ( P