Objective To investigate the effect of electroacupuncture on expression of growth associated protein-43 (GAP-43) and Nogo-A in brain after focal cerebral ischemia/reperfusion in rats. Methods 48 male Sprague-Dawley rats were randomly divided into sham group, model group and electroacupuncture group, and the latter two were modeled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture. Electroacupuncture was performed 90 min after modeling in the electroacupucture group at acupoints of Neiguan (PC06), Shuigou (DU26), Sanyinjiao (SP06), Baihui (DU20), for 30 min, once a day. The sham group and the model group were conventionally fed in cages without any intervention. 8 rats of each group were assessed with Longa's score, and the expressions of GAP-43 and Nogo-A were detected with immunohistochemistry 7 d and 14 d after modeling respectively. Results The sham group presented no neurological symptoms. There was not different in Longa's score between the model group and the electroacupuncture group 7 d after modeling (P>0.05), but was different 14 d after modeling (P<0.05). GAP-43 positive cells was not found in the sham group, but could be found around cerebral ischemia 7 d after modeling, which decreased 14 d after modeling in the model group. GAP-43 positive cells increased significantly in the electroacupuncture group compared with the model group at each time (P<0.01). Nogo-A positive cells was few found in the sham group, and was more in the model group (P<0.01). The expression of Nogo-A decreased significantly in the electroacupuncture group compared with the model group at each time (P<0.01). Conclusion Electroacupuncture can improve neurological function of focal cerebral ischemia/reperfusion rats, which may be associated with the increase of GAP-43 and descrease of Nogo-A in peri-infarct regions

This paper introduced the approach and practice of merging the standardized resident training with the master degree candidates training in Chongqing.A comparison of the performance and pass rate of the completion examinations between the standardized training of specialist residents in 2014 and those of merged training for both residents and master degree candidates in 2015 in Chongqing,analyzed the patterns and differences of the two training programs.Based on such,the authors proposed to improve the combined training mechanism,by completing leadership and rules,enhancing quality control and completing the appraisal systems.

BACKGROUND:Angiotensin II receptor antagonists have been found to exerct a stronger protective effect on bone than angiotensin converting enzyme inhibitors. OBJECTIVE:To investigate the effect of different concentrations of irbesartan (angiotensin II receptor antagonist) on the differentiation and mineralization of mouse preosteoblasts. METHODS:Mouse preosteoblast cel lines MC3T3-E1 in logarithmic phase were selected and cultured in the osteogenic induction medium containing 0 (control group), 0.001, 0.01, 0.1 mmol/L irbesartan, respectively. Ten days later, the cel differentiation was observed by alkaline phosphatase staining. The mineralization was observed by alizarin red staining after 21 days of culture. mRNA expressions of osteocalcin, alkaline phosphatase and Runt-associated transcription factor 2 in osteoblasts were detected by real-time PCR at 1, 4, 7, 14 and 21 days of culture. RESULTS AND CONCLUSION:The activity of alkaline phosphatase in al the irbesartan groups (0, 0.001, 0.01, 0.1) was higher than that in the control group (P<0.05), which was the most obvious in 0.01 mmol/L. The number and area of calcium nodules in each irbesartan group were significantly higher than those in the control group (P<0.05), especial y in 0.01 mmol/L. Compared with the control group, 0.01 mmol/L irbesartan significantly upregulated the mRNA expressions of osteocalcin, alkaline phosphatase and Runt-associated transcription factor 2 (P<0.05). These results suggest that 0.01 mmol/L irbesartan significantly promotes the differentiation and mineralization of osteoblasts.

Objective To investigate the cognition and attitude of check-up system in Standardized Residents Training Program in Chongqing, so as to provide further suggestions to improve teachers' qualifi-cation and examination management system. Methods From September 2016 to December, a questionnaire survey was conducted among 265 teachers and 59 teachers in Chongqing, which includes basic information of teachers and their cognition, satisfaction and suggestion of residents training examination management system. SPSS 21.0 was used to describe the survey results and the Spearman rank correlation analysis was conducted. Results 265 valid questionnaires were recovered, and the effective recovery rate was 100％. The survey showed that the teachers' cognition of resident training examination management system is good. Only 21 people (8％) completely had no knowledge of the management system of resident related assessment. A total of 90.6％ of the clinical mentors considered that a check-up system of clinical mentors on training should be implemented strictly during the period of clinical mentors on training. Only 167 (63％) of the hos-pitals had developed detailed assessment system for resident physicians. Conclusion Most of the teachers in Chongqing have a higher awareness of living and training management system, and it is universally recog-nized for strict process assessment. However, in terms of the standardized training examination system for residency, it needs improvement. It is suggested that the examination system should be perfected, the ex-amination management be strengthened, and the teachers' team be standardized.

Objective:To understand the current situation and influencing factors of depression among residents in Chongqing, and to explore the relationship between depression and administration satisfaction of the standardized residency training.Methods:Stratified random cluster sampling was used to select 372 residents currently participating the standardized residency training in Chongqing. The patient health questionnaire (PHQ-9) and administration satisfaction of residency training questionnaire were used to conduct a cross-sectional survey on the depression status and satisfaction of residents in Chongqing. SPSS 21.0 software package was used for partial correlation analysis and binary logistic regression analysis to explore the relationship between depression and administration satisfaction of the standardized residency training.Results:Among the 372 residents, 218 had mild depression and above (58.60%). There was a significant difference in the incidence of depression among residents of different ages, educational background, marital status, working hours and administration satisfaction of residency training ( χ2 = 23.02, 17.35, 12.24, 21.70, 95.20; P = 0.028, 0.027, 0.016, 0.041, <0.001 respectively). Partial correlation analysis showed that there was a negative correlation between the total score of depression and the five aspects of the administration satisfaction (total score, instructors, training base, managers and training income) ( r=-0.52, -0.45, -0.47, -0.45, -0.40, all P<0.01). In the binary logistic regression analysis of depression and satisfaction,the satisfaction had a negative impact on depression, and its B value was -1.71, and OR value was 0.18, P<0.001. Conclusion:The incidence of depression among residents in Chongqing is high. Age, educational background, marital status, working hours and administration satisfaction are related to the degree of depression. And the higher the degree of depression, the lower the administration satisfaction of residents. Relevant departments should pay attention to the mental health of residents and improve the quality of standardized residency training.

Objective:To understand the current situation of medical humanistic quality of standardized trainees for hospitalizations in Chongqing, and put forward the corresponding educational countermeasures and suggestions. Methods: According to the method of convenient sampling, 5 resident doctors standardized training base were selected with half a random, then 110 trainees were randomly selected to conduct a cognition investigation about the medical humanistic quality. EpiData 3. 0 was adopted for double entry data and SPSS22. 0 software was used for analyzing data. Results:There were 98. 06％ of standardized trainees for hospitalizations paid more attention to medical humanistic quality. In the selection of medical humanistic quality courses, 81. 55％ of the trainees chose The Doctor-patient Relationship, 77. 67％ of the trainees chose The Medical Psychology. In the evaluation of the importance of medical humanistic quality to the clinical work, 96. 12％ of the standardized trainees for hospitalizations believed that it was important. Conclusion: In order to improve the medical humanistic quality of the standardized trainees for hospitalizations, all aspects should be taken seriously:it should make efforts from the trai-nees' self-awareness, the teaching teachers' attention to the medical humanistic quality and the medical humanis-tic quality environment provided by the training base.

【Objective】 To investigate the current situation concerning volume control of red blood cells in additive solution produced by blood service in Chongqing, and to lay a foundation for promoting the homogenization of preparation process of red blood cells in additive solution. 【Methods】 A questionnaire was designed to investigate the factors related to the preparation of red blood cells in additive solution. The questionnaire was sent by Chongqing Association of Blood Transfusion via E-mail to 18 blood services in the city, and the collected data was sorted, revised and analyzed by research team. 【Results】 A total of 18 blood services(including 1 blood center + 1 sub-center, 6 central blood stations and 11 central blood banks) returned the questionnaires. The results showed that there were differences among blood services across Chongqing, regarding the centrifugal parameters during preparation, the operation mode and monitoring situation of the capacity control during preparation, and the formulation of the capacity standard of red blood cells in additive solution etc. 【Conclusion】 The preparation process of red blood cells in additive solution, produced by Chongqing blood services, should be further standardized, and the capacity control method of this product in Chongqing should be gradually unified to achieve regional homogeneity and to ensure blood safety.

【Objective】 This study endeavors to introduce the statistical process control (SPC) method to analyze the quality control index concerning red blood cells in additive solution with leukocytes reduced, with the aspiration to advance the effective utilization of blood quality control data, thereby providing empirical foundations for the continual enhancement of blood quality. 【Methods】 Between 2020 and 2022, test data pertaining to the quality control index of red blood cells in additive solution with leukocytes reduced were amassed from six blood stations in Chongqing area. Utilizing Minitab software, the SPC analysis was carried out, p-control charts were delineated, the non-conformance rates of each quality control index along with their 95% confidence intervals were computed, as well as the Process Capability Index (Z value). 【Results】 In accordance with the Whole Blood and Blood Components Quality Requirements, the appraisal of the quality control indexes for red blood cells in additive solution with leukocytes reduced manifested a conformity rate of 100% for appearance, end-of-storage hemolysis rate and sterility test. Nonetheless, the conformity rates for volume, hemoglobin, hematocrit and residual leukocytes did not attain 100%, albeit all were ≥75%. Through the employment of binomial distribution-based p-control charts, the controlled state of the production process was discerned. Although the overarching conformity rate satisfied the national standard stipulations, it was discerned that there were out-of-control points concerning volume, hemoglobin, hematocrit, and residual leukocytes across different institutions, exhibiting palpable trends. The non-conformance rates of all quality control indexes were less than 25%, yet at a 95% confidence level, the residual leukocyte counts from institutions B, C, E, and F did not adhere to the stipulations (exceeding 25%). By architecting the ability evaluation index Z value for count data process capability analysis, it was unveiled that the volume of institution E, the hematocrit of institutions B, C, and F, and the residual leukocytes Z values of all six blood collection and supply institutions were below 2, hinting at avenues for amelioration. 【Conclusion】 The SPC method anchored in binomial distribution exhibits substantial application merit in blood component quality management, facilitating real-time surveillance of blood collection, preparation, and storage procedures.