1.The effect of budesonide inhalation suspension via transnasal nebulization to the volume of bleeding during endoscopic sinus surgery.
Jinyan XING ; Xinguo LIU ; Yongyuan TIAN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(14):1077-1078
Administration, Inhalation
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Adolescent
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Adult
;
Aged
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Blood Loss, Surgical
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Budesonide
;
administration & dosage
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Child
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Endoscopy
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Humans
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Middle Aged
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Nose
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surgery
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Young Adult
2.Culture and identification of rat basilar artery smooth muscle cells
Qinying QIU ; Jiaguo ZHOU ; Yujie LIU ; Yongyuan GUAN
Chinese Pharmacological Bulletin 2003;0(11):-
Aim To investigate the method of cell culture for smooth muscle cells from rat cerebral basilar artery and understand cells growth and biological characteristics.Methods The explant attached method was applied for cell culture of rat basilar artery smooth muscle cells(BASMCs).The cultured BASMCs were identified by immunocytochemical staining.The activities of cells were indicated by the dynamic changes of intracellular calcium concentration observed by RF-5 000 fluorospectro-photometer.Results BASMCs grew out of tissue blocks by 5 days,reached confluency could be subcultured after 2 weeks.Cultured cells were identified by intensely positive immunocytochemical staining to smooth muscle actin-specific.Introduction of calcium channel agonists induced significant increase in Fura-2 fluorescence ratio(F340/F380)and cells were in good condiction.Conclusion Explant attached method is simple,efficient and economic.It provides an ideal cell model for the study of pathogenesis of the cerebral vascular diseases.
3.Evaluation of molluscicidal effect of screening snails in soil layer
Yongyuan LIU ; Haigen XU ; Yihe HU ; Zhenqiu ZHU
Chinese Journal of Schistosomiasis Control 2009;21(6):574-
Both the snail number and the detection rate of living snails by means of snail screening in soil layer were higher than those of snail survey on soil surface. It is indicated that snail screening in soil layer can make up the shortage of survey on soil surface and improve the efficiency of snail control.
4.Experiment of Oncomelania hupensis snail reproduction and susceptibility in schistosomiasis non-endemic area in Southern Jiangsu
Xuedong WANG ; Xinfeng CHEN ; Feng WU ; Fei YUAN ; Zhenqiu ZHU ; Yihe HU ; Yongyuan LIU
Chinese Journal of Schistosomiasis Control 2010;22(2):182-184
Objective To observe the survival and reproduction of Oncomelania hupensis snails and their susceptibility to schistosome in schistosomiasis non-endemic area of the Yangtze River estuary in Southern Jiangsu.Methods The soil and water from the Yangtze River estuary of Southern Jiangsu were used for the experiment.The snails reproduced in the same year were collected from the upper reaches of the Yangtze River and raised in the laboratory.The snail survival and reproduction rates and schistosome infection of the snails were observed.The soil collected from schistosomiasis endemic area was used in the control group.Results There was no significant difference between the experimental and control groups for the snail survival rates both in 6 and 12 months (X~2= 0.727 8,P > 0.05 and X~2 = 0.416 1,P > 0.05).Each female snail reproduced 67.69 eggs in average (95 % CI:24.026 0-110.097 4).The average hatchability rate of snail eggs was 83.60%,and there was no significant difference between the 2 groups (X~2= 9.131 8,P > 0.05).The schistosome infection rate of the second generation snails was 1.40% (5/356) 60 days after the infection in the laboratory.Conclusions The snails collected from the upper reaches of the Yangtze River marshland can survival and reproduce in the soil and water from schistosomiasis non-endemic area of the Yangtze River estuary of Southern Jiangsu,and the second generation of the snails can be infected with schistosome in the laboratory.
5.Relationship between echocardiographic epicardial adipose tissue thickness and coronary artery disease
Changchun LAI ; Yuefeng TONG ; Yongyuan XU ; Zhixing HU ; Yunxiang WANG ; Zhecheng LI ; Weijun Lü ; Zehua LIU
Chinese Journal of Ultrasonography 2010;19(9):757-760
Objective To evaluate the relationship between echocardiographic epicardial adipose tissue thickness(EAT) and the presence and severity of coronary artery disease(CAD). Methods One hundredand forty-seven patients (101 patients with CAD and 46 patients with normal coronary arteries by diagnostic coronary angiography) were enrolled. EAT thickness was measured using 2-D echocardiographic parasternal long-and short-axis views. EAT thickness measurements were compared with angiographic findings. Results EAT was significantly higher in CAD group comparison to control group [(7.41 ± 1.63)mm vs (4.41±1.60) mm, P <0.01 ]. Furthermore, EAT increased with the severity of CAD [(8.53 ± 1.00)mm vs (6.36 ±1.73)mm, P <0.01]. Gensini's score significantly correlated with EAT (r = 0.71, P <0.01 ). EAT thickness ≥5.35 mm had 87.13% sensitivity and 80.42% specificity (ROC area 0. 89, P = 0.01,95% CI [0.84 - 0.9;]) for predicting CAD. Conclusions EAT thickness, which is easily and non-invasively evaluated by transthoracic echocardiography, can be an adjunctive marker to classical risk factors for the prediction of CAD, it was significantly correlated with the severity of coronary artery disease.
6.Atorvastatin Inhibits The Development of Macrophages to Foam Cells
Peng WU ; Ying-Feng LIU ; Yun-Qin CHEN ; Donghui LIANG ; Xiu-Li ZHANG ; Yongyuan CHEN ; Wen-Zhong CHEN ;
Chinese Journal of Hypertension 2007;0(04):-
Objective To investigate effects of atorvastatin on the development from macrophages (HMDM) to foam cells.Methods Monocytes were isolated from human peripheral blood by Ficoll-Hypaque density gradient centrifugation and plastic adsorptive process.The isolated cells were stimulated by phorbol 12-myristate 13-acetate (PMA) (50 nmol/L) for 48 h and transformed to macrophages.Macrophages were co-incubated with 80 mg/L ox- idized low density lipoprotein (ox-LDL) and atorvastatin (0-100 ?mol/L),respectively for 0,6,12 and 24 h. Total cholesterol (TC),free cholesterol (FC) and protein (Pro) in cultured cells were quantitatively analyzed by high performance chromatography (HPLC) analysis and modified Lowry protein assay.Results When macropha- ges were incubated with 80 mg/L ox-LDL,the ratio of TC/Pro was greater than 20,and large amount of lipid drop- lets were displayed indicating the formation of foam cells.Atorvastatin decreased TC/Pro ratio in foam cells in a concentration and time dependent manner (0-100 ?mol/L)(P
7.CT diagnosis and surgical treatment of adhesive ileus
Hongjie LIU ; Haoran SUN ; Yongyuan LI ; Weiguang HE ; Pei YANG ; Ying ZHOU ; Zonghui GAO ; Chunzhong MA
Chinese Journal of General Surgery 2018;33(1):57-60
Objective To analyze CT findings in adhesive intestinal obstruction caused by different adhesion pattern.Methods Clinical data of 83 adhesive intestinal obstruction cases proved by laparotomy were reviewed.Before surgery two experienced abdominal radiologists randomly double blindly inspected and evaluated the CT findings,as to whether there was intestinal obstruction,the severity of obstruction,the site of obstruction,the adhesion type of obstruction and the diagnosis of strangulated intestinal obstruction,the consensuses were accomplished by discussion.Results The accuracy of diagnosis was 100% for the existence of obstruction,the severity of obstruction and the site of obstruction.There were intra-abdominal hernia caused by intestinal adhesion (44 cases),a cluster of loop (12 cases),bowel twisted angle (8 cases),the intestine and abdominal adhesions (6 cases),intestinal adhesion contracture stenosis (5 cases),adhesive band compression (4 cases),intestinal volvulus caused by adhesion (1 case).The accuracy,sensitivity,specificity,positive predictive value and negative predictive value of CT in diagnosing strangulated intestinal obstruction were 78%,47%,100%,100%,73%,respectively.Conclusion CT can clearly diagnose different adhesion pattern leading to ileus and ensuing strangulation.
8.Contrast enhanced ultrasonography vs.contrast enhanced computed tomography for the diagnosis of focal lesions of the pancreas
Zimei LIN ; Minqiang PAN ; Yongyuan XU ; Qing WEN ; Chunmei LIU ; Yao WANG ; Caoxin YAN ; Qinghai LI ; Pintong HUANG
Chinese Journal of General Surgery 2018;33(10):849-852
Objective To assess the characterization and usefulness of contrast enhanced ultrasound (CEUS) to diagnose focal lesions of pancreas in comparison to contrast enhanced computed tomography (CECT).Methods 177 cases of focal solid lesions of the pancreas confirmed by pathology were collected.The enhanced patterns and diagnostic capability of CEUS were analyzed,and these results were compared with those of CECT.Results The diagnostic accuracies of ultrasound,CEUS and CECT were 61.6% (109/177),89.3% (158/177),and 92.1% (163/177),respectively.There was no significant difference between the diagnostic accuracies of CEUS and CECT (P =0.071).The sensitivity,specificity,positive predictive value (PPV),negative predictive value (NPV),and accurate rate for CEUS were 90.9%,63.6%,73.8%,86.2%,82.5% (the areas under the curve,AUC =0.820),and 89.8%,69.3%,76.7%,85.9%,84.7% for CECT (AUC =0.847),respectively,when the hypo-enhanced was regarded as the cut value.No significant difference was found between these results from CEUS and CECT (P =0.071).Conclusions The diagnostic ability of CEUS in focal lesions of the pancreas is similar to that of CECT.
9. Application of contrast enhanced ultrasound in TN staging of pancreas cancer: comparison with contrast enhanced computed tomography
Zimei LIN ; Qing WEN ; Yongyuan XU ; Chao ZHANG ; Caoxin YAN ; Guoqiang MO ; Minqiang PAN ; Chunmei LIU ; Pintong HUANG
Chinese Journal of Ultrasonography 2018;27(7):614-617
Objective:
To assess value of contrast enhanced ultrasound (CEUS) in TN staging of pancreatic cancer and compared with contrast enhanced computed tomography(CECT).
Methods:
Seventy-eight cases with pancreatic cancer confirmed by pathology were enrolled in this study. All patients were examined using CEUS and CECT and staged according to the 8th guideline of pancreas tumors of AJCC. The diagnostic accuracies of CEUS in TN staging of pancreas tumors were compared with CECT.
Results:
The diagnostic accuracies of CEUS in T staging and N staging of pancreatic cancer were 80.8%, and 78.2%, respectively. For CECT, the diagnostic accuracies in T staging and N staging were 88.5%, and 88.5%, respectively. There was no significant difference in the diagnostic accuracies between CEUS and CECT in T staging(χ2=1.56,