1.Research in correlations between occupational pressure, social support and subjective well-being
Xuedan YU ; Jing LI ; Yongyu GUO
Chinese Journal of Practical Nursing 2012;28(33):64-66
Objective To research in correlations between occupational pressure,social support and subjective well-being,in order to explore effective ways to reduce the occupational pressure.Methods Nurses from a certain grade 2A hospital in Wuhan were selected as research objects.Questionnaires were used to investigate the correlations between occupational pressure,social support and subjective well-being.Results Nurses endured a high level burden.The subjective well-being of nurses was negatively correlated with occupational pressure,the higher the occupational pressure,the lower the subjective well-being.The subjective well-being of nurses was positively correlated with social support.The occupational pressure and social support could predict the subjective well-being of nurses.Conclusions There exists potential relationships between occupational pressure,social support and subjective well-being of nurses.This results supports evidence for nursing managers to cultivation and management of nurses,which is helpful to establish effective nursing manage-ment measures.
2.Superparamagnetic iron oxide and chlormethylbenzamido-1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine labeling and tracing bone marrow mesenchymal stem cells
Xingkuii DOU ; Tao GUO ; Yongyu SI ; Linjun WAN ; Qingqing HUANG ; Bo KANG
Chinese Journal of Tissue Engineering Research 2010;14(14):2513-2517
BACKGROUND:The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo.Superparamagnetic iron oxide(SPIO)labeling is an ideal magnetic resonance contrast medium,and it offers the potential for non-invasive tracking of implanted cells.The chlormethylbenzamido-1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (CM-Dil)labeling does not have cytotoxicity,so it cannot influence cell growth.OBJECTIVE:To investigate the effects of SPIO and CM-Dil labeling and tracking on bone marrow mesenchymal stem cells(BMSCs).METHODS:Porcine BMSCs were isolated and cultured by the whole bone marrow method.BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-Dil.The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion.The frozen sections of the cardiac tissues were obtained after 4 weeks.RESULTS AND CONCLUSION:Efficiency of SPIO and CM-Dil labeling BMSCs was nearly 100%.The SPIO and CM-Dil labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation.SPIO and CM-Dil labeling BMSCs were efficiently tracked in vivo.