Objective To explore the expression of THBS 2 and its clinical significance in gastric cancer . Methods The mRNA and protein expression levels of THBS2 were assessed in 14 paired of gastric cancer specimen and corre-sponding normal mucosa using quantitative real-time PCR and Western blot analysis. Immunohistochemistry of TH-BS2 on a population-based tissue microarray consisting of 129 gastric cancer cases was used for evaluating the TH-BS2 prognostic significance. Kaplan-Meier method and Cox′s proportional hazards model were used in survival anal-ysis. Results Both mRNA and protein expression of THBS2 in 12 gastric cancer tissues were remarkably lower than the corresponding normal tissues among total 14 pair tissues. Consistent with the results of our Western blot, THBS2 expression was significantly inhibited in gastric cancer tissues as that in the normal controls in TMA ( P=0. 031) . Overexpression of THBS2 had a significant correlation with favorable prognosis of gastric cancer patients ( P=0. 002 ) and decreasing THBS2 expression was associated with the poor histological grade of gastric cancer his-tological grade ( P=0. 005 ) . Conclusion This study suggests THBS2 is down-regulation in gastric cancer tissue versus normal gastric tissue, and it may play a critical role in gastric cancer carcinogenesis and may be a potential prognosis predictor of gastric cancer.

Objective To investigate the feasibility and safety of radical resection of colorectal carcinoma with laparoscopy.Methods Clinical data of 48 patients who underwent laparoscopic colorectal cancer resection and 131 patients who underwent traditional radical resectin were retrospectively analyzed.Results The incision length,first flatus time,intestinal intervention times,the first meal time,urinary catheterization time,gastric tube retaining time,drainage tube lien time,analgesia time of the laparoscopic group significantly decreased compared with those of conventional surgery ( P ＜ 0.05 ).There was no significant difference in complication occurrence,postoperative hospitalization time and overall hospitalization time between the two groups( P ＞ 0.05 ).The average operation time of laparoscopic surgery is longer than that of the traditional group (P ＜ 0.05 ).There was no significant difference between 2 groups in regarding of perioperative bleeding rate,volume of intraoperative and postoperative blood transfusions,mass maximal diameter,the distance between the distal and proximal margin and the mass in colon specimens,as well as the two variables in rectum specimens,the resected lymph node number and positive lymph node number (P ＞0.05).Conclusion Laparoscopic resection for colorectal cancer,a safe and feasible technology,is of the same short-term curative effect with traditional open surgery,but the follow-up radical curative effect is yet to be confirmed.

Objective　To investigate the role of splenomegaly and splenic macrophage(MΦ)in the occurrence of the hypersplenism of portal hypertension(PHT).Methods　The changes of the quantity of ACP in MΦ,the ulstrastructure of MΦ and the quantity of the blood cells phagocytized and destructed by MΦ in the spleen of PHT by the methods of immunohistochemistry and transmission electron microscope were observed and compared with the normal spleen.The correlation of spleen index,the quantity of ACP in MΦ and the peripheral blood cells counts were also studied.Results　Comparing with the normal spleen,the quantity of ACP in MΦ was increased,the surface folopodia of MΦ was increased and lengthened and the quantity of lysosome in MΦ and the quantity of erythrocytes and platelets phagocytized by MΦ were increased.Spleen index and the quantity of ACP in MΦ were negatively correlated with the peripheral blood cells counts,spleen index was positively correlated with the quantity of ACP in MΦ.Conclusion　The splenomegaly and splenic MΦ could play a vital role on the occurrence of the hypersplenism of PHT.

Objective To investigate the role of splenic macrophage(M) in the occurrence of hypersplenism in patients with portal hypertension(PHT). Methods The changes in the quantity, distribution, ultrastructure of M, the quantity acid phophatase in M ,and the quantity of M phagocytizing blood cells were determined by immunohistochemistry and transmission electron microscopy in the normal and PHT spleen. Results Compared with normal spleen, the cell density of the M in PHT spleen decreased;and the quantity of acid phophatase in M increased; the surface folopodia of M increased and lengthened;and the quantity of lysosome in M and the quantity of M phagocytizing blood cells increased. Conclusions The splenic M may play an important role in the occurrence of hypersplenism in PHT.

Objective To develop a method for the determination of acrylamide in flocculant by gas chromatography-mass spectrometry.Methods Acrylamide was derived through bromination and extracted by organic solvent.GC-MS method was used to identify the target substance and quantify acrylamide.Results The recoveries obtained from samples spiked with standards were in the range of 70.2%-109.2% and relative standard deviations (n=5) was less than 5.0%.Linearity of peak area was obtained over a wide range of concentration (4.3-2 000 ?g/ml),and the correlation coefficients was 0.999 4.Conclusion The method is reliable,selective and reproducible and it is applicable to the determination of the acrylamide in flocculant.

Objective To investigate the safety and validity of Neptune 3D-RTPS-A treatment planning system compared to Prowess TPS.Methods A total of 30 clinical tumor cases with radiotherapy planning on Prowess TPS from September 2009 to May 2010 were used.The contours, organs at risk and target volumes in Prowess TPS were transported into Neptune TPS, the same parameters setted in the two treatment planning systems.The results of comparison of the two TPS were calculated.Results All cases of clinical treatment planning were completed successfully by Neptune TPS, and the various functions of the design were achieved for fitting tumor conformal radiation therapy.The key parameters on radiation treatment were compared.The results are as follows:the differences of source skin distance ( SSD ) ＜0.5% , differences of Monitor Unites ＜0.5%, the differences of dose at isocenter ＜2%, the differences of five isodose lines surrounding area ＜ 3%, and the mean difference of distances of five isodose lines was 0.43 mm, the differences of the volume of PTV on 90% isodose line ＜ 2%, and the differences in V30of organs at risk ＜ 3%.Conclusions Neptune TPS could be qualified for clinical validity and safety by clinical verification.

Objective　To obtain the normal ranges of different parameters in static posturography.Methods　Different parameters' values of 117 normal persons (10～19 years old) were detected by static posturography while they were in opening and closing eyes situations. Results　The mean values of different parameters in opening eyes situation are significantly lower that in closing eyes situation(P＜0.01). The values from the male and the female demonstrate no significant difference, values from 10～19 years old group and 70～79 years old group are significantly higher than from 20～69 years old group(P＜0.01). Conclusion　The static posturography is a perfect measure to analyze and evaluate the balance ability of human body in exact quantification, and could be one of the routine clinic tests, supplying quantificational, intellective and facile method for diagnosis and evaluation of dizziness and balance ability.

Objective To explore significance and impact of hypoxia inducible factor 1α,metastasis associated with colon cancer 1 and transcription factor FOXO4 in gastric carcinoma and clinical pathological characteristics and prognosis .Methods 110 samples diagnosed as gastric carcinoma and 46 samples diagnosed as pericarcinomatous tissue which was 5 cm far away from cancer were collected in first affiliated hospital of Anhui medical university from December 2006 to May 2008 .Immunohistochemical stai-ning was performed in these samples to detect the expressions of HIF-1α,MACC1 and FOXO4 .And the correlation between devel-opment of gastric carcinoma and expressions of HIF-1α,MACC1 and FOXO4 was analyzed with the help of patients′messages .Ka-plan-Meier method and Cox regression were used to analyze patients′5 years survival rate and the factors .Results The expression levels of HIF-1α(P<0 .001) and MACC1(P<0 .001) in gastric carcinoma tissues were significantly higher than pericarcinomatous tissues ,however the expression levels of FOXO4(P<0 .001) was significantly decreased in gastric carcinoma tissues .Statistical a-nalysis revealed that the expressions of HIF-1αand MACC1 were apparently increased with the lower degree of cell differentiation , the greater degree of tumor infiltration ,accompanied with lymph node metastasis and higher TNM scores ,but lowered the expres-sion of FOXO4 factor .There were significant correlations between expressions of the three factors in gastric carcinoma .The expres-sions of HIF-1α,MACC1 and FOXO4 had an apparent influence on the patients′5 years survival rate .And they were independent factors on the prognosis of gastric carcinoma .Conclusion HIF-1α,M ACC1and FOXO4 are abnormal expression in gastric cancer tissue ,and the clinical pathological features of gastric cancer and the prognosis of patients with gastric cancer are closely related .

Objective To investigate the effects of laparoscopic and open resection with pelvic autonomic nerve preservation (PANP) on sexual function of male patients with lower rectal cancer. Methods Total 177 male patients with lower rectal cancer received surgery from September 2008 to December 2013 were enrolled into two groups: the laparoscopic PANP group (n = 105) and the open PANP group (n = 72). The classifications of erectile and ejaculatory functions were used to evaluate the sexual functions of patients at 6 months and 12 months post-operation, respectively. The effect of different operation on the sexual function of the male patients was compared between the two groups. Results The incidence rates of erectile dysfunction at 6 months and 12 months post-operation in the laparoscopic group were lower than those in the laparotomy group (P < 0.05). The incidence rates of ejaculatory dysfunction at 6 months and 12 months post-operation in the laparoscopic group were also lower than those in the laparotomy group (P < 0.05). Conclusion The laparoscopic resection with PANP in patients with lower rectal cancer can not only clearly reveal pelvic autonomic nerve and effectively protect them, but also reduce the incidence of postoperative sexual dysfunction.