OBJECTIVE: To study the hemostatic effects of Qishu gongxue granules. METHODS: Kunming mice and Wistar rats were randomly divided into five groups, i.e. normal control group, Fuke zhixuening group and Qishu gongxuening granules high-dose, medium-dose and low-dose groups. The change of coagulation time was compared among five groups. The value of PT and APTT were determined. Platelet aggregative function in rats was detected. RESULTS: Qishu gongxuening granules could reduce coagulation time of model mice and the level of PT and APTT in rats while it enhanced platelet aggregative function in rats. CONCLUSION: Qishu gongxuening granules can reduce coagulation time and it can be used for uterine bleeding. Hemostatic mechanism is associated with the production of plasmozyme and thrombokinase in the coagulation of blood and platelet aggregative function.

Objective To investigate the effects of 8Hz infrasound on spatial learning ability of rats and its the underlying mechanism. Methods Forty male SD rats were randomly divided into a control group and an experiment group (consisting of the 8Hz, 90dB, 100dB and 130dB subgroups). The experimental subgroups were exposed to 8Hz, 90dB, 100dB or 130dB infrasound for 2 hours daily, respectively, for a total of 6 days. All of the rats were taken out of the infrasonic depot once a day and given Morris maze training. And at last the brain of the rats were harvested and treated with immunohistochemical staining for detection of ryanodine receptor (RyR). The content of RyR in hippocampi and temporal cortex were detected under an optical microscope. Results The rats in the 8Hz, 90dB, 100dB and 130dB subgroups showed prolonged latency in Morris training ( P =0.010, P =0.001 and P = 0.000 ). The hippocampi and temporal cortex of rats in 8Hz, 90dB, 100dB and 130dB subgroups showed less positive neurons of RyR ( P =0.000). There was a negative correlation between the latency period of rats finding the hidden platform and the content of RyR in hippocampus and temporal cortex. Conclusion Infrasound of 8Hz, 90dB, 100dB and 130dB could decrease the spatial learning ability of rats, and this was possibly related to the reduce of RyR in hippocampus and temporal cortex.

Objective To study the effect of 8Hz infrasound on the expression of 5-HTR of hippocampus and temporal cortex in rats. Methods SD rats were exposed to 8Hz, 90dB,100dB or 130dB infrasound, respectively for 2 hours daily. Then the rats' brains were taken and given stained immunohistochemically for (5-hydroxy tryptamine receptor,5-HTR). The content of 5-HTR in hippocampus and temporal cortex were detected under an optical microscope. Results The animals in the infrasound groups had less expression of 5-HTR in hippocampus and temporal cortex, and the least were at the 35th day for 90dB group and 100dB group and the 28th day for 130dB group (P

OBJECTIVE:To investigate the utilization of antineoplastic drugs and adjuvant drugs in our hospital. METHODS: By a retrospective review, the consumption quantity, consumption sum, and constituent ratio of antineoplastic drugs and adjuvant drugs used in our hospital from 2005 to 2008 were analyzed statistically. RESULTS: The consumption of antineoplastic drugs and assistant drugs had dominated the front places from 2005 to 2008, representing 25.91%, 28.16%, 31.16% and 33.00%, respectively of the total western drugs in terms of consumption sum, assuming a year-on-year increase tendency over the 4 years. Injections took a higher proportion, making up for 75.40%, 79.80%, 80.50% and 78.12%, respectively over the 4 years. The application of antineoplastic drugs and assistant drugs from domestic manufacturers assumed a predominant place. CONCLUSION: It is still necessary to standardize the use of antineoplastic drugs and assistant drugs in our hospital so as to ensure their safety, effectiveness and economy in clinical medication.

Objective To invesitgate the relationship between 8-iseprostane (8-iso-PG) level in exhaled breath condensates (EBCs) and severity of asthma and explore the role of 8-iso-PG in asthma evaluation and monitoring.Methods Fifty-nine patients with asthma were enrolled.In which 15 eases were acute exacerbation, 13 eases were mild intermittent, 15 eases were mild persistent, and 16 eases were mederate-to-severe persistent.Thirteen healthy volunteers were recruited as control.EBCs were collected using EeoSereen system.The 8-iso-PG levels in EBCs were measured by a specific enzyme immunoassay.The patients with mild intermittent asthma were treated with inhaled corticosteroid (ICS) for one month and their EBCs were recollected for 8-iso-PG measurement.Results Exhaled 8-iso-PG levels were obviously increased in the patients with acute asthma compared with those chronic asthmatics [(47.2±6.8) pg/mL vs (24.5±12.0) pg/mL, P < 0.01].In the chronic persistent asthma, the levels were significantly higher in patients with mild persistent and moderate-to-severe asthma [(17.9±1.2) pg/mL and (39.7±4.0) pg/mL, P <0.01].While 8-iso-PG level did not differ significantly in intermittent asthma [(13.5±1.1) pg/mL] compared with the control subjects (P > 0.05).After one-month ICS treatment the 8-iso-PG level in the patients with mild intermittent asthma did not change significantly although the ACT score improved.Conclusions 8-iso-PG levels in EBC are associated with the severity of asthma,implicating 8-iso-PG may be useful in monitoring airway oxidative stress in asthma.ICS treatment is incapable of decreasing the 8-iso-PG,suggesting the ICS has minor impact on oxidative stress.

OBJECTIVE:To establish the method for simultaneous determination of 6 components in Lonicera japonica,and to study the content changes of them before and after before and after carbonized. METHODS:UPLC method was adopted. The deter-mination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisting of 0.1% phosphoric acid solu-tion-acetonitrile(gradient elution)at the flow rate of 0.2 mL /min. The detection wavelength was set at 350 nm,and column tem-perature was 25 ℃. The sample size was 1 μL. RESULTS:The linear ranges of chlorogenic acid,rutin,galuteolin,isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C were 21.2-424 μg(r=0.9993),1.17-23 μg(r=0.9995),2.18-43 μg(r=0.9998),5.10-102 μg(r=0.9993),2.60-52 μg(r=0.9991),4.95-99 μg(r=0.9998),respectively. RSDs of precision,stability and repeatability tests were all lower than 2.0%. Recoveries were 97.11%-99.76%(RSD=1.20%,n=6),95.20%-99.90%(RSD=2.20%,n=6),95.71%-100.30%(RSD=2.20%,n=6),95.00%-96.98%(RSD=0.88%,n=6),96.47%-103.00%(RSD=2.40%, n=6),95.78%-103.80%(RSD=3.20%,n=6). Compared with before processing,the contents of rutin,isochlorogenic acid B and isochlorogenic acid C in L. japonica were increased along with processing,the contents of chlorogenic acid and isochlorogenic acid A were decreased significantly,while the content of galuteolin had no significant change. CONCLUSIONS:The method is sim-ple,precise,stable and repeatable,and can be used for simultaneous determination of 6 components in L. japonica. Those chemi-cal components have certain changes before and after carbonized.

Objective To investigate the risk factors of cognitive impairment in post-intensive care syndrome patient (PICS-CI).Methods A retrospective study was conducted. The patient who transferred from post-intensive care unit (ICU) to the general ward for more than 7 days, and with the age ≥ 18 years old in the First Affiliated Hospital of Jinzhou Medical University from October 2015 to November 2016 were enrolled. The gender, age, marital status, education, occupation, salary, economic income, smoking, alcohol drinking, previous of history, mechanical ventilation, the length of ICU stay, sedative and delirium, and initial diagnosis were recorded. According to mini-mental state examination (MMSE), the patients were divided into cognitive impairment (CI) group and non-CI group. Univariate analysis was performed to identify the risk factors of PICS-CI, and variables with statistical difference were selected to do multivariate binary logistic regression analysis for the confirmable independence risk factors.Results 104 of the 290 patients developed CI, and the incidence was 35.86%. Univariate analysis showed that the gender, age, education, financial situation, smoking, alcohol drinking, previous of history, mechanical ventilation, the length of ICU stay, sedative and delirium, and initial diagnosis were recorded of ICU were main predictors of PICS-CI patients. It was shown by multivariate binary logistic regression analysis that the age > 60 years old [odds ratio (OR) = 7.523, 95% confidence interval (95%CI) = 2.572-37.851,P = 0.001], mechanical ventilation (OR = 8.773, 95%CI = 2.588-36.344,P < 0.001), sedation (OR = 9.376, 95%CI = 2.661-42.011,P = 0.002), and delirium (OR = 13.201, 95%CI = 2.502-41.433, P < 0.001) were PICS-CI independence risk factors.Conclusions Nurse staffs should strength care and attention on ICU transferred out patients. In order to minimize PICS impairment, special precaution should be implemented according to different aspects.

Objective To compare the difference of IL-8 and MMP-9 levels in the serum and sputum before and after treatment in patients with acute exacerbations of COPD,and to analyze the correlation between IL-8 and MMP-9 levels and lung function.Method A total of 20 cases of AECOPD patients were randomly selected in the observation group and 15 cases of healthy people were selected in the control group.Pulmonary function was measured and the specimens of sputum and blood were collected during the admission and discharge for AECOPD patients.FEV1 and FEV1/FVC％ were recorded,and the concentration of IL-8 and MMP-9 in the sputum supernatant and serum were tested with double antibody sandwich enzyme linked immunosorbent assay before and after treatment.All the data and correlation were analyzed with t test and SPSS software.Results (1) Before the treatment,serum IL-8 concentration in COPD patients was 28.30 ± 14.39 pg/mL,higher than that in the control group [17.46 ± 3.48) pg/mL,P=0.008].After treatment,the serum IL-8 concentration was significantly decreased [(13.30 ± 6.31) pg/mL,P=0.000].IL-8 concentration of sputum significantly decreased after treatment [(501.60 ± 508.61) pg/mL,P=0.042] than one before treatment (639.40 ± 703.12) pg/mL.(2) Before treatment,serum MMP-9 concentration in patients with AECOPD was 1.12 ± 0.36 ng/mL,lower than control group [(17.89 ± 11.00) ng/mL,P=0.000)].After treatment,the serum MMP-9 concentration was significantly decreased [(0.87 ± 0.31) ng/mL,P=0.000].MMP-9 concentration of sputum before treatment (12.04 ± 12.22) ng/mL decreased significantly after treatment [(8.02 ± 7.13) ng/mL,P=0.005].(3) Before treatment,no correlation was seen between the concentration of serum IL-8 and FEV1,FEV1/FVC.Negative correlation was observed between the sputum IL-8 concentration and FEV1/FVC and no correlation was found with FEV1.After treatment,we found that no correlation showed between the concentration of IL-8 and FEV1,FEV1/FVC,and negative correlation was seen between the IL-8 concentration in sputum and FEV1,FEV1/FVC.(4) Before treatment,no correlation between the concentration of serum MMP-9 and FEV1,FEV1/FVC was observed and no correlation between the sputum MMP-9 concentration with FEV1 was seen.Negatively correlation was found between the sputum MMP-9 level wtih FEV1/FVC.After treatment,we observed no correlation between the serum concentration of MMP-9 and FEV1,FEV1/FVC,and negative correlation between the concentration of MMP-9 in sputum and FEV1,FEV1/FVC.Conclusion The concentration of IL-8 and MMP-9 in serum and sputum in patients with acute exacerbation of COPD is higher than those in the stable phase.IL-8 and MMP-9 levels in serum show no correlation with lung function.Negative correlation was observed between IL-8 and MMP-9 levels in sputum and lung function.We can test the levels of IL-8 and MMP-9 in sputum to evaluate the severity of the disease.

Objective To study the changes of Toll like receptor 4,7 (TLR4,TLR7) protein in cervical squamous cell carcinoma ( CSCC) tissues and the relationship between expression and clinical pathological characteristics of the patients. Methods A total of 67 pa-tients with CSCC carcinoma( CSCC group) ,30 patients with cervical intraepithelial neoplasia( CIN group) and 30 patients with normal cervi-cal tissues(normal group) were detected by immunohistochemical method. The expression of TLR4,TLR7 protein and its clinical pathological characteristics of patients with CSCC in the samples were analyzed. Results TLR4,TLR7 protein positive rate of CSCC group were respec-tively 80. 60% and 76. 12%,which were significantly higher than those of CIN group (46. 67%,40. 00%) and the normal group(13. 33%, 10. 00%),the difference was statistically significant (P<0. 05). TLR4,TLR7 protein positive expression rate in CIN group was significantly higher than that in the normal group (P<0. 05). The positive expression of TLR4 protein and CSCC in patients with FIGO stage,pathological grade,depth of invasion had a significant relationship(P<0. 05),pathological grade,the positive expression of TLR7 protein and CSCC in pa-tients with invasive depth had a significant relationship(P<0. 05). Conclusion There is increased expression of TLR4,TLR7 protein in cervical tissues of CSCC patients,and a certain relationship between the factors and clinical staging,pathological grading.

Objective To investigate the different distribution and expression of mammalian target of rapamycin complex (mTORC) in the kidney of diabetic nephropathy (DN) mice.Methods Fourteen eight-week-old male C57BL/6 mice were assigned to 2 groups: the control group ( n=7 ) and the streptozotocin ( STZ )-induced DN group ( n=7 ) . Blood and urinary variables including glucose , albumin, creatinine and albumin/creatinine ratio were assessed 2 weeks after STZ injection.Hematoxylin-eosin staining was performed for renal pathological analyses .The distributions of mTOR , phosph-ser2448-mTOR(p-mTOR), mTORC1(Raptor), mTORC2(Rictor) and phosph-ser240/244-S6K1 (p-S6K1) were determined by immunofluorescence.The expression levels of mTOR, p-mTOR, mTORC1(Raptor), mTORC2(Rictor), S6K1 and p-S6K1 were detected by Western blotting .Results Two weeks after STZ injection , the diabetic mice developed albuminuria (P<0.01) and renal hypertrophy (P<0.05).The immunofluorescence positive staining for mTOR , Raptor, and Rictor was distributed in the epithelial cells of proximal tubules , glomerular mesangium and capillary loops as well as the medullary collecting ducts of the control mouse kidney .These positive signals increased in the DN mouse kidney ( P<0.05).However, pS6K1 was not detected in the inner medulla of control mouse and p-mTOR was not found in the glomeruli of both control and DN mice .Conclusion mTORC is widely expessed in the mouse kidney and participates in the development of DN , whereas the 2448 serine phosphorylation of mTOR may be not implicated in the hyperglycemia mediated glomerular injury .