This study was aimed to establish quality standards of fruit of Hippophae tibetana Schlechtend.. The medical material was identified by microscopy and thin layer chromatography (TLC). According to methods recorded in the Chinese Pharmacopoeia (2010 edition), the contents of moisture, total ash, acid-insoluble ash and alcohol-sol-uble extract were determined. The HPLC method was applied in the determination of quercetin, kaempferol and isorhamnetin content. The results showed that the microscopic characteristics of Tibetan medicine H. tibetana were identified. The TLC and content determination methods of quercetin, kaempferol and isorhamnetin were estab-lished. It was concluded that the established qualitative and quantitative methods can be used for quality control of the fruit of H . tib e tana.

This study was aimed to investigate the comprehensive ecological factors of Hippophae rhamnoides L. and their regional suitability in China. Based on field survey, specimen examination and literature investigation, ecologi-cal factors and appropriate production areas were analyzed by Traditional Chinese Medicine Geographic Information System (TCMGIS-II). The results showed that the proper region (with similarity of 95%~100%) of H. rhamnoides L. accounts for 737 994.71 km2, including 15 provinces/municipalities and 387 counties/cities. The largest area among them is Tibet autonomous region with area of 313 857.73 km2 (42.53%), followed by Sichuan province (223 987.02 km2, 30.35%), Gansu province (66 314.43 km2, 8.99%) and Shanxi province (4 237.79 km2, 0.57%). There are also certain appropriate production areas distributed in Liaoning province, Beijing, Chongqing and Hubei province. It was concluded that this system is much valuable to the recognition of the formation of the producing area, the division of adaptive area, introduction and acclimatization of medicinal materials. It also provided a scientific reference for the introduction and cultivation of H. rhamnoides L. Through further field study and experiments, these new areas have the potential to be developed into suitable production region of H. rhamnoides L. in the future.

OBJECTIVETo explore material basis of the pharmacological differences between the roasting and pro-roasting Raphani Semen.

METHODThe two new sulfur-containing compounds (A209 and B221) were found changed after processing in the water decoction. The common precursor-C3 of A209 and B211 and the precursor of C3 were seperated and purified. Their transforming relationship was proved.

RESULTThe result showed that glucosinolates could decompose into sulforaphane and transform into A209 and B221 further in the boiling process.

CONCLUSIONThis study provides some experimental evidences for revealing the mechanism of Raphani Semen processing.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Glucosinolates ; chemistry ; isolation & purification ; metabolism ; Isothiocyanates ; Seeds ; chemistry ; enzymology ; Sulfur Compounds ; chemistry ; Thiocyanates ; chemistry

BACKGROUND: Lung cancer is the main cause of cancer-related death globally. Single nucleotide polymorphism (SNP) is one of the important factors leading to the occurrence of lung cancer, but its mechanism has not been elucidated. This study intends to investigate the relationship between SNPs of CDH1, FANCB, APC genes and lung cancer genetic susceptibility. METHODS: The case-control study design was used. We collected blood samples from 270 lung cancer cases in the Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, as well as blood samples from 445 healthy volunteers as controls, and extracted genomic DNA for genotyping using the Taqman® SNP genotyping kit. The distribution of three SNP loci of CDH1 gene rs201141645, FANCB gene rs754552650 and APC gene rs149353082 in Chinese population was analyzed. Chi-square test and Logistic regression were used to analyze the relationship between different genotypes and the risk of lung cancer. RESULTS: The distribution frequencies of AA, A/G and GG genotypes at rs754552650 of FANCB gene in the control group were 27.2%, 52.6% and 20.2%, respectively. The distribution frequencies of AA and A/G genotypes were 93.7% and 6.3% in the case group, respectively, and no GG genotype was detected. The A/G genotype of the rs754552650 locus of the FANCB gene was significantly different between the case group and the control group. Compared with the carriers of AA genotype, the individuals with FANCB rs754552650 A/G genotype had a lower risk of lung cancer (OR=0.035, 95%CI: 0.020-0.062, P<0.001). CDH1 gene rs201141645 A/C and CC genotypes only existed in the control group. In addition, only 1 sample was found to have APC rs149353082 genotype in the case group. CONCLUSIONS In the Chinese population, the lung cancer risk of the individuals with FANCB rs754552650 A/G genotype was significantly decreased.
Antigens, CD/genetics* ; Cadherins/genetics* ; Case-Control Studies ; China ; Fanconi Anemia Complementation Group Proteins/genetics* ; Gene Frequency ; Genes, APC ; Genetic Predisposition to Disease ; Genotype ; Humans ; Lung Neoplasms/genetics* ; Polymorphism, Single Nucleotide