1.Simultaneous Determination of Bavachin and Bakuchiol in Psorslea corylifolia by HPLC
Xiaojing HUANG ; Xiaoying ZHOU ; Yongsheng WEN
China Pharmacy 2017;28(24):3400-3402
OBJECTIVE:To establish a method for content determination of bavachin and bakuchiol in Psoralea corylifolia.METHODS:HPLC method was adopted.The determination was performed on Shim-Pack VP-ODS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 246 nm,the column temperature was 30 ℃,and the sample size was 10 μL.RESULTS:The linear ranges of bavachin and bakuchiol were 48.9-489 ng(r=0.999 8),784-7 840 ng(r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 2.0%.Average recoveries ranged 95.30%-99.63% (RSD=1.45%,n=9),96.89%-100.82% (RSD=1.36%,n=9).CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for the simultaneous determination of bavachin and bakuchiol in P corylifolia.
2.Investigation on the Effect of Jieduquyuziyin Methods on Decreasing the Dosage of Glucocorticoid and Protecting the Hypothalamic-pituitary-adrenal Axis in Systemic Lupus Erythematosus
Yongsheng FANG ; Chengping WEN ; Yongwei LI
Journal of Zhejiang Chinese Medical University 2006;0(02):-
0.05),while with significant difference in the second therapy course(P
3.Determination of Ursodesoxycholic Acid in Compound Capsules of Bear Gall Ethylic
Yongsheng WEN ; Zhiming XU ; Ling LI ;
Chinese Traditional Patent Medicine 1992;0(03):-
Objective: To determine the content ursodesoxycholic acid of Compound Capsule of Bear Gall thylic. Methods: TLCS method was used. Results: Owing to improving the method on the base of original Criterion, the determination result is more similar to the true value.
4.Biomechanical quantitative study in hallux valgus deformity associated with the second metatarsalgia
Jianmin WEN ; Haiwei HU ; Yongsheng SUN
Chinese Journal of Orthopaedics 2000;0(02):-
Objective To study the relation between the pressure in forefoot and the second metatarsalgia in hallux valgus deformity, finally to quantitatively analyze the pressure under the second metatarsal head which is a dangerous factor leading to second metatarsalgia. Methods Three groups volunteered for this study by a self-devised foot pressure measurement system: fifty-eight normal subjects (116 feet) without pain and deformity in foot, thirty-two patients (45 feet) with hallux valgus and pain only under the second metatarsal head, fifty-six patients (102 feet) with hallux valgus without symptoms of metatarsalgia. The percentage of pressure to weight was the criteria for data analysis. First the pressures under second metatarsal head among three groups were compared with each other. Secondly we used case-control study to identify whether it was high pressure that was likely to result in metatarsalgia. Finally we compared the pressure interval under second metatarsal head among three groups to define the pressure interval which might be a dangerous factor to second metatarsalgia. Results 1)The pressure beneath the second meatatarsal head in patients with metatarsalgia was the highest in the three groups (P
5.Influence of Treating Principles of Detoxication - Stasis Removing - Yin - Nourishing on Hemorheology in Cases of Lupus Erythematosus
Yongsheng FAN ; Chengping WEN ; Huanpeng GU
Journal of Traditional Chinese Medicine 1993;0(06):-
By combining clinical observation and animal experiments and using FASCO - 3010 hemorheological devices to examine the relevant indices, it was found that no obvious changes can be found in the group treated with hormone before and after the treatment, whereas in the group with traditional drugs added, the RBC deformation, and specific viscosity of plasma were all markedly lowered. Comparison of rheological changes in SD rat showed that traditional Chinese drugs can markedly improve the hemorheology in such patients, indicating that these TCM treating principles can improve the concentrated, viscous, coagulated, agglutinated pathological blood status in SLE patients.
6.Effects of Jiedu Quyu Ziyin Recipe on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr mice
Lingyong CAO ; Zhijun XIE ; Xinchang WANG ; Chengping WEN ; Yongsheng FAN
Chinese Journal of Primary Medicine and Pharmacy 2010;17(6):733-735
Objective To explore the effects of Jiedu Quyu Ziyin Recipe (JQZR) on the apoptosis and expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte in MRL/lpr mice. Methods 80 MRL/lpr mice were randomly divided into model group,TCM group, Western medicine group and TCM and Western medicine group,20 mice in each group, meanwhile,20 Kunming mice were selected as normal group, then intragastrically administered normal sodium, JQZR apozem, prednisone suspension and JQZR apozem and prednisone suspension, 0. 5ml every time,once daily for 12 weeks respectively. At the end of the 12th week, peripheral-blood lymphocytes of every mice purified by gradient centrifugation were cultivated for 48 hours ,then the apoptosis was detected by flow cytometry. Furthermore,the expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte were detected by RT-PCR. Results At Oh or 48h,the apoptosis ratios of PBLC in normal group, TCM group,Western medicine group and TCM and Western medicine group are higher than model group and the differences are significant(P <0. 05 ,P <0. 01 ) ,while,at Oh,which are not significant within Western medicine group, normal group and TCM and Western medicine group ( P > 0. 05) ,even if which are significant between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group( P < 0. 05 ). At 48h, the differences of apoptosis ratios of PBLC are significant between Western medicine group and TCM and Western medicine group( P < 0. 05 ), and between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group which are significant, too( P < 0. 01 ). The ratios of bcl-2/bax mRNA of normal group,TCM group and TCM and Western medicine group are significantly lower than which of model group or Western medicine group ( P < 0. 05, P < 0. 05, P <0. 01 ,respectively) ,but there is no significant difference between which of model group and Western medicine group.Conclusion JQZR has adjustable effects on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr Mice.
7.Clinical analysis of tracheotomy of severe craniocerbral trauma in 42 cases
Guanmin HUANG ; Yongsheng ZHOU ; Wen LIU ; Yebin ZHANG ; Fei LU
Chinese Journal of Primary Medicine and Pharmacy 2008;15(7):1081-1082
Objective To explore the significance, indicatinos and complications of emergency tracheotomy for patients with severe craniocerbral trauma. Methods The clinical data of 42 patients with severe craniocerbral trauma were retrospectively analyzed. Results Of them, 20 were basically cured (48%), 12 had improvement (29%) ,3 were released from the hospital(7 % ), 1 survived(2 % ) in a vegetative state,and 6 died(14%). Conclu- sion Emergent tracheotomy should be performed on patients with severe craniocerbral trauma to resolve respiratory tract obstruction, improve air current, prevent cerebral anoxia, prevent and treat pulmonary infection, increase the therapeutic efficacy.
8.Simultaneous Determination of 3 Components in Xiaoer Huadu Powder by HPLC
Zhen ZENG ; Guangli AI ; Tingting LI ; Yongsheng WEN ; Shiyu ZHOU
China Pharmacy 2017;28(24):3405-3408
OBJECTIVE:To establish a method for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.METHODS:HPLC method was adopted.The separation was performed on Waters SunFireTM-C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 238 nm,and column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate were 8.808-88.08 μg/mL(r=0.999 8),1.778-17.78 μg/mL(r=0.999 6),2.533-25.33 μg/mL(r=0.999 9),respectively.LOQ were 4.404,0.889,2.533 μg/mL;LOD were 1.101,0.445,1.267 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2%.The recoveries were 95.08%-99.61% (RSD=1.77%,n =9),96.93%-99.94% (RSD=0.92%,n=9),98.33%-102.05% (RSD=1.27%,n=9).CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for simultaneous determination of paeoniflorin,berberine hydrochloride and ammonium glycyrrhizinate in Xiaoer huadu powder.
9.Study on quality standard for Qiankun Liquid
Yongsheng WEN ; Shiyu ZHOU ; Ling LI ; Yuxin HE ;
Chinese Traditional Patent Medicine 1992;0(06):-
Objective: To establish the quality standard for Qiankun Liquid.Methods: Fructus Lycii、Fructus Cnidii、Radix Ginseng in Qiankun Liquid were identified by TLC, and the content of icariin was determined by HPLC.Results: Fructus Lycii、Fructus Cnidii、Radix Ginseng could be identified by TLC. Icariin showed a good linear relationship at a range of 0.126~1.008?g, r =0.9999. Conclusion: The method is accurate and can be used for the quality control of Qiankun Liquid.
10.Investigation into Preventive and Therapeutic Effects of Jieduquyuziyin Methods in Treating Corticosteroid Osteoporosis in Systemic Lupus Erythematosus
Yongsheng FAN ; Chengping WEN ; Guolin WU ; Al ET ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(11):-
; Objective; To observe the preventive and therapeutic effects of Jieduquyuziyin methods in treating corticosteroid osteoporosis in systemic lupus erythematosus (SLE) and investigate into its mechanism. Method; 147 cases of SLE were randomly divided into the integrated traditional Chinese and western medicine group (79 cases) and the western medicine group (68 cases). There 21 cases which were diagnosed primarily among all 147 cases. 30 healthy cases were taken as the normal control group. The western medicine group (68 cases) was treated mainly with prednisone only, while the integrated traditional Chinese and western medicine group (79 cases) with Jieduquyuyiyin methods on the basis of normal western medicine. Both groups were treated for 3 months as a course. 16 cases in the western medicine group and 25 cases in the integrated traditional Chinese and western medicine group were kept on being treated and observed for 2 years. The lever of serum Ca, P, ALP, BGP, IPTH, 25-(OH) D3 and urinary Ca, P and BMD were checked and observed. Result; The levers of serum P, 25-(OH) D3, BGP and BMD in the normal group, primary diagnosis group, integrated traditional Chinese and western medicine group were higher than those in the western medicine group significantly ( P