Objective To evaluate the therapeutic effect of plasmid encoding interleukin 4 (pIL-4) administered via gene-gun delivery and intradermal injection on collagen-induced arthritis (CIA). Methods IL-4 plasmid was administered by gene-gun delivery and intradermal injection to DBA/1 mice immunized with type Ⅱ collagen (CⅡ). Its effect on the development of CIA was evaluated clinically with the visual analogue scoring method for arthritis and serologically by enzyme linked immunosorbent assays (ELISA) and polymerase chain reaction. Results Treatment with IL-4 plasmid could significantly reduce the incidence and severity of CIA including a reduction in the anti-CⅡ antibody level. Gene-gun delivery had a stronger immunosuppressive effect on CIA compared with intradermal injection. As shown by in vitro stimulation assay, the speen cells from mice immunized with CⅡ and treated with IL-4 plasmid via gene-gun exhibited higher Th2 cytokine responses compared with cells treated with control plasmid after in vitro stimulation with CⅡ. Conclusion The results of this study suggest that treatment with IL-4 plasmid may constitute a new approach of cytokine gene therapy for rheumatoid arthritis.

Objective To study the potential effects of telomerase inhibitor on the apoptosis of bladder cancer cells. Methods A phosphorothioate oligonucleotide (PS ODN) with sequence 5′ d(TTAGGG) 3′, as the telomerase inhibitor, was incubated with a bladder cancer cell line EJ. Such treated cells were studied with many approaches for the telomerase activity, the growth status and the morphological changes. Results The PS ODN inhibited the telomerase activity in the cells, arrested the cell growth, and induced the apoptosis of the cells. Conclusions The apoptosis of bladder cancer cells could be induced by PS ODN with 5′ d(TTAGGG) 3′ sequence as a telomerase inhibitor.

Objective To study the telomerase activity in the tissue adjacent to bladder cancer and to investigate its clinical significance. Methods Telomerase activity was detected with telomeric repeat amplification PCR (TRAP) assay. The telomerase activity in the tissue adjacent to bladder cancer was evaluated. Results Telomerase activity was positive in the tissue samples adjacent to bladder cancer in 10 of the 24 cases(42%). Telomerase activity in the adjacent tissue has been related with the tumour grades and stages. The tumour recurrence was also related with the telomerase activity in the adjacent tissue. Conclusions The detection of telomerase activity in the tissue adjacent to bladder cancer could be a prognostic marker for bladder tumor recurrence.

Objective To evaluate the manifestations of glenohumeral instability on CT arthrography.Methods 16 cases of glenohumeral instability were examined both by CT arthrography and arthroscopy and to analyze the manifestations on CT arthrography.Results Intraarticular injury included labral tear, capsular stripping or laxity, anterior glenoid fracture and lateral posterior humeral head impacted fracture.Conclusion Shoulder arthrography has good contrast, and because the imaging no overlaps, can clearly show the intraarticular injures. It is a good examine method of glenohumeral instability.

Objective To explore the curative effects, side effects and complications of arterial embolization for the treatment of uterine leiomyoma.Methods Bilateral uterine arterial embolization with silk particles of 350～500 ?m was performed in 16 cases of uterine leiomyomas with uterine hemorrhage as the main complaint.Results 15 patients underwent technically successful embolization. No serious complications occurred. The patients were followed up for 3～12 months. Menses returned to regular cycle. In the patients with anemia, the hemoglobin concentration rose up to normal level. The sizes of uterine leiomyoma decreased by 52% after 6 months of the treatment.Conclusions Uterine arterial embolization is an effective and less invasive therapy with no serious complications for patients with uterine leiomyoma, especially for presentation of the uterus.

Objective To investigate the influential factors of cognitive impairment after lacunar brain infarction(LBI).Methods The neuropsychological tests including mini-mental state examination(MMSE) scale,clock-drawing test(CDT),execute function(EF)in ADAS-cog were applied in 136 LBI patients(LBI group) and 60 gender,age and concomitant chronic diseases matched controls(control group).Those results were compared between the two groups.The influence of the LBI patients's age,the number of cerebral lesion and concomitant disease(hypertension,diabetes,hyperlipidemia) on the scores of the above tests were analyzed.Results(1)The incidence of cognitive impairment in LBI group(55.8%) was distinctly higher than that in control group(15%);the scores of total MMSE and CDT in LBI group were significantly lower than those in control group;while the EF score was higher(all P

Objective To investigate the effect of neoadjuvent chomotherapy of paclitexel plus eisplatin for improving the surgical resection rate of stage ⅢA non-small cell lung cancer(NSCLC).Methods 46 patients with stage ⅢA NSCLC were randomly divided into neoadjuvant chemotherapy group(25 caeses) and simple operative group(25 cases).Neoedjuvant chemotherapy group(NCG) received two-cycle chemotherapy and then underwent operation.Simple operative group(SOG) was only treated by operation.Results The operative resection rate of NCG was 99.0%,radical resection rate was 64.0%.It was higher obviously than that simple operative group(SOG)(P

Objective To study the long-term results of 90 cases with inoperable stage Ⅲ non-small cell lung cancer.Methods All patients received CAP regimen or MVP regimen chemotherapy.80 patients received radiotherapy.Results The results showed that 1-,3-,5-year survival rates were 53.4%,19.5% and 8.5% respectively.The 3-,5-year survival rate for squamous cell carcinoma were higher than those adenocarcinoma(P

Objective To report secondary paroxysmal sympathetic hyperactivity in a patient with tuberculous meningitis and to review the diagnostic criteria, clinical features, possible pathogenesis and management of this condition. Methods The clinical data of a case with paroxysmal sympathetic hyperactivity secondary to tuberculous meningitis was retrospectively analyzed and related literature was reviewed. Results A 1-year-old boy was admitted to our institute with a history of lethargy and vomiting for 3 days. Neurological examination revealed abnormalities. A lumbar puncture revealed the evidence of meningitis. PPD test, T-SPOT.TB and radiological examination revealed tuberculous meningitis. Later, when stayed in the intensive care unit, he developed paroxysmal hypertension, sinus tachycardia, tachypnea, dystonia, and high fever. These episodes improved after administration of propranolol, benzodiazepines and artane. Conclusions Paroxysmal sympathetic hyperactivity is a rare manifestation of tuberculous meningitis, early recognition is very important for avoid misdiagnosis and overtreatment.

Objective To investigate clinical biologic characteristics and factors that impact prognosis of patients with follicular thyroid carcinoma(FTC).Method Clinical data of 105 FTC patients treated surgically in the Department of Head& Neck,Tianjin Cancer Hospital from 1970 to 1990 was analyzed retrospectively.Results The overall 5-year,10-year and 15-year survival rates of these patients were 85.3％,76.7％ and 72.9％ respectively.The overall 15-year survival rates of patients ≥45 years and those ＜ 45 years were 45.9％,89.8％ respectively.The overall 15-year survival rates of patients with unilateral and bilateral carcinoma was 50.0％,76.2％ respectively.The overall 15-year survival rates of patients with neck lymph node metastasis and without were 54.2％,79.2％ respectively.The overall 15-year survival rates of patients at stage Ⅰ,stage Ⅱ,stageⅢ and stageⅣ were 89.3％,70.0％,45.5％ and 35.3％respectively.During the follow-up period,11 patients were diagnosed with distant metastasis from 1 year to 33 years after surgical treatment and 9 died of cancer within 5 years after diagnosis.Seventeen patients had local recurrence from 3 months to 34 years after surgery and 10 of these patients died of local recurrence.Conclusions The factors influencing prognosis of patients with FTC were age,clinical stage,bilateral carcinoma and neck lymph node metastasis,therefore early treatment and close following-up are essential to improve the prognosis of patients with FTC.