Objective To evaluate the therapeutic effect of plasmid encoding interleukin 4 (pIL-4) administered via gene-gun delivery and intradermal injection on collagen-induced arthritis (CIA). Methods IL-4 plasmid was administered by gene-gun delivery and intradermal injection to DBA/1 mice immunized with type Ⅱ collagen (CⅡ). Its effect on the development of CIA was evaluated clinically with the visual analogue scoring method for arthritis and serologically by enzyme linked immunosorbent assays (ELISA) and polymerase chain reaction. Results Treatment with IL-4 plasmid could significantly reduce the incidence and severity of CIA including a reduction in the anti-CⅡ antibody level. Gene-gun delivery had a stronger immunosuppressive effect on CIA compared with intradermal injection. As shown by in vitro stimulation assay, the speen cells from mice immunized with CⅡ and treated with IL-4 plasmid via gene-gun exhibited higher Th2 cytokine responses compared with cells treated with control plasmid after in vitro stimulation with CⅡ. Conclusion The results of this study suggest that treatment with IL-4 plasmid may constitute a new approach of cytokine gene therapy for rheumatoid arthritis.

Objective To investigate the influential factors of cognitive impairment after lacunar brain infarction(LBI).Methods The neuropsychological tests including mini-mental state examination(MMSE) scale,clock-drawing test(CDT),execute function(EF)in ADAS-cog were applied in 136 LBI patients(LBI group) and 60 gender,age and concomitant chronic diseases matched controls(control group).Those results were compared between the two groups.The influence of the LBI patients's age,the number of cerebral lesion and concomitant disease(hypertension,diabetes,hyperlipidemia) on the scores of the above tests were analyzed.Results(1)The incidence of cognitive impairment in LBI group(55.8%) was distinctly higher than that in control group(15%);the scores of total MMSE and CDT in LBI group were significantly lower than those in control group;while the EF score was higher(all P

Objective To study the potential effects of telomerase inhibitor on the apoptosis of bladder cancer cells. Methods A phosphorothioate oligonucleotide (PS ODN) with sequence 5′ d(TTAGGG) 3′, as the telomerase inhibitor, was incubated with a bladder cancer cell line EJ. Such treated cells were studied with many approaches for the telomerase activity, the growth status and the morphological changes. Results The PS ODN inhibited the telomerase activity in the cells, arrested the cell growth, and induced the apoptosis of the cells. Conclusions The apoptosis of bladder cancer cells could be induced by PS ODN with 5′ d(TTAGGG) 3′ sequence as a telomerase inhibitor.

Objective To study the telomerase activity in the tissue adjacent to bladder cancer and to investigate its clinical significance. Methods Telomerase activity was detected with telomeric repeat amplification PCR (TRAP) assay. The telomerase activity in the tissue adjacent to bladder cancer was evaluated. Results Telomerase activity was positive in the tissue samples adjacent to bladder cancer in 10 of the 24 cases(42%). Telomerase activity in the adjacent tissue has been related with the tumour grades and stages. The tumour recurrence was also related with the telomerase activity in the adjacent tissue. Conclusions The detection of telomerase activity in the tissue adjacent to bladder cancer could be a prognostic marker for bladder tumor recurrence.

Objective To evaluate the manifestations of glenohumeral instability on CT arthrography.Methods 16 cases of glenohumeral instability were examined both by CT arthrography and arthroscopy and to analyze the manifestations on CT arthrography.Results Intraarticular injury included labral tear, capsular stripping or laxity, anterior glenoid fracture and lateral posterior humeral head impacted fracture.Conclusion Shoulder arthrography has good contrast, and because the imaging no overlaps, can clearly show the intraarticular injures. It is a good examine method of glenohumeral instability.

Objective To explore the curative effects, side effects and complications of arterial embolization for the treatment of uterine leiomyoma.Methods Bilateral uterine arterial embolization with silk particles of 350～500 ?m was performed in 16 cases of uterine leiomyomas with uterine hemorrhage as the main complaint.Results 15 patients underwent technically successful embolization. No serious complications occurred. The patients were followed up for 3～12 months. Menses returned to regular cycle. In the patients with anemia, the hemoglobin concentration rose up to normal level. The sizes of uterine leiomyoma decreased by 52% after 6 months of the treatment.Conclusions Uterine arterial embolization is an effective and less invasive therapy with no serious complications for patients with uterine leiomyoma, especially for presentation of the uterus.

Objective To investigate the effect of neoadjuvent chomotherapy of paclitexel plus eisplatin for improving the surgical resection rate of stage ⅢA non-small cell lung cancer(NSCLC).Methods 46 patients with stage ⅢA NSCLC were randomly divided into neoadjuvant chemotherapy group(25 caeses) and simple operative group(25 cases).Neoedjuvant chemotherapy group(NCG) received two-cycle chemotherapy and then underwent operation.Simple operative group(SOG) was only treated by operation.Results The operative resection rate of NCG was 99.0%,radical resection rate was 64.0%.It was higher obviously than that simple operative group(SOG)(P

Objective To study the long-term results of 90 cases with inoperable stage Ⅲ non-small cell lung cancer.Methods All patients received CAP regimen or MVP regimen chemotherapy.80 patients received radiotherapy.Results The results showed that 1-,3-,5-year survival rates were 53.4%,19.5% and 8.5% respectively.The 3-,5-year survival rate for squamous cell carcinoma were higher than those adenocarcinoma(P

Objective To evaluate the efficacy and safety of dorsal root entry zone (DREZ) incision for pain induced by brachial plexus injury. Methods A cohort study was performed in 41 consecutive patients with pain induced by brachial plexus injury treated with DREZ incision from July 2005 to December 2007. Of all, 19 patients were amputated and had phantom limb sensation. Oral pain relief (0%-100%) and complications were followed up by one professional doctor. Oral pain relief rate ≥50% was considered satisfactory outcome. Results The proportion of patients with satisfactory pain relief was 90% (37/41), 81% (30/37), 77% (24/31) and 70% (16/23) at 2 weeks, 3 months, 6 months and last follow-up (over one year) after surgery. At last follow-up, 15 out of 16 patients with over 10 years of pain duration got satisfactory pain relief; while only 16 out of 25 patients with less than 10 years of pain duration got satisfactory pain relief (x2 =4.682, P =0.030). In 16 amputees who were followed up for more than three months, seven patients with alteration of phantom limb sensation got satisfactory pain relief, while only four out of nine patients without alteration of phantom limb sensation got satisfactory pain relief. Among 37 patients followed up for more than three months, severe neurological defects were found in three patients including one with mild motor disturbance in the ipsilateral lower limb and two with severe sensory disturbance in the ipsilateral lower limb. Conclusions DREZ incisions are effective and safe neurosurgical procedure for relieving pain induced by brachial plexus injury. The possible predictive factors of better outcome are the long preoperative pain duration and postoperative phantom limb sensation among amputees.

Objective To explore treatment methods and prognosis of external ventricular drainage .Methods A retrospective analysis of 66 cases with severe intraventricular hemorrhage patients were selected ,36 cases underwent bilateral ventricle drainage and urokinase ( UK ) intraventricular fibrinolysis therapy +terminal cistern drainages , 30 cases with bilateral ventricle drainage and urokinase for intraventricular fibrinolysis .The prognosis of the patients with modified Rankin Scale score was assessed .After multiple cerebral CT examination hematoma imaging changes were observed.Results A group of intraventricular hemorrhage clearing time (5.3 ±1.5)d,B group of intraventricu-lar hemorrhage clearing time (8.2 ±2.5)d,there was significant difference (t=-5.820,P=0.000).Rate in group A was 83.3%(30/36),group B was 66.7%(20/30), there was no significant difference (χ2 =2.475,P=0.116). Conclusion This study suggests that intraventricular hemorrhage were given bilateral ventricle drainage and uroki -nase for intraventricular fibrinolysis therapy +terminal cistern drainages effect is better .