Objective To compare the effect of dynamic hip screw(DHS) and anatomical locking plate for treatment of femoral intertrochanteric fractures.Methods 100 patients with intertrochanteric fractures were divided into observation group and the control group.The observation group was treated with DHS fixation,the control group was treated with anatomic locking plate.The therapeutic effect,incision length,blood loss,postoperative drainage,operative time,hospitalization time and complication rate were compared between two groups.Results The excellent,good,fair,poor,and the excellent and good rate in observation group were 35 cases,9 cases,5 cases,1 case and 86％,those in the control group were 37 cases,10 cases,3 cases,0 case and 94％.There was no significant difference in the excellent and good rate between two groups (x2 =1.77,P ＞ 0.05).Operation incision length,bleeding volume,postoperative drainage volume and operation time values of the observation group were better than those in the control group,the differences had statistical significance(t =4.44,10.91,9.52,9.73,all P ＜ 0.05).The hospitalization time and incidence rate of postoperative complications between two groups had no statistically significant differences (P ＞0.05).Conclusion In the treatment of intertrochanteric fractures,DHS fixation and anatomic locking plate fixation have good effects,anatomic locking plate fixation method has the advantages of small incision,less bleeding and shorter operative time.

The approach to the further development of organ transplantation in China was investigated. The analysis on the siuation of organ transplantation in China and the faced difficulties demonstrated that the people's traditional cultural background,ideas and the ethnics mental view were the factors responsible for the development of organ transplantation in China lt was proposed that changing opinions,implementation of brain death method and legislation lf organ transplantation were the fundamental way to promote the development of organ transplantation in China and get rid of the difficulty of the modern medicine

OBJECTIVE:To study the effects of mifepristone on the expression of inflammatory factors in endometriosis (Ems)model rats. METHODS:SD rats were randomly divided into normal group(normal saline),model group(normal saline), mifepristone low-dose,medium-dose and high-dose groups [0.65,1.30,2.60 mg/(kg·d)],with 10 rats in each group. Except for normal group,Ems model was induced in other groups,and they were given relevant medicine intragastrically for consecutive 4 weeks. The volume of the ectopic focus was compared before and after treatment. The serum contents of TNF-α,IL-6 and IL-8 were detected by ELISA method. The phosphorylation of NF-κB p65 and the expression of COX-2 and PGE2 were detected by West-ern blot. The expression of NF-κB p65 mRNA in ectopic focus was detected by RT-PCR. RESULTS:Compared with normal group,the volume of the ectopic focus,the serum contents of TNF-α,IL-6 and IL-8,the expression of COX-2 and PGE2 protein, the phosphorylation of NF-κB p65 and the expression of NF-κB p65 mRNA in ectopic focus were increased in model group(P<0.01). Compared with model group,the volume of the ectopic focus,the serum contents of TNF-α,IL-6 and IL-8,the expression of COX-2 and PGE2 protein,the phosphorylation of NF-κB p65 and the expression of NF-κB p65 mRNA in ectopic focus were de-creased in mifepristone groups(P<0.01),in dose-dependent manner. CONCLUSIONS:Mifepristone can reduce the volume of the Ems ectopic focus,via blocking NF-κB signaling pathway and reducing the expression of inflammatory factors.

Chimeric antigen receptor T cells (CAR-T), one of the most promising cancer immunotherapy, has attracted much attention in the 57th American Society of Hematology (ASH) annual meeting. CAR-T therapy has obtained significant effect on leukemia and lymphoma and the latest research results are also inspiring in the 57th ASH annual meeting. It is an important task that how to combine CAR-T therapy with the traditional methods of treatment and the immune checkpoint blocking antibodies and small-molecule-targeted drugs to achieve the best effect. This paper will review the progress of CAR-T in the treatment of hematologic malignancies.

With the further study on heterogeneity and molecular mechanism of diffuse large B-cell lymphoma (DLBCL),many novel targeted drugs have been developed,which also showed clinical benefit in early-stage studies.Therapeutic targets for DLBCL mainly include the surface antigens of B lymphocyte,B cell receptor signaling and the cellular microenvironment.Several new clinical trials about targeted therapy for DLBCL had been reported in the 55th ASH annual meeting,and the results were encouraging.The advances in this field will be summarized in this paper based on the new reports in the 55th ASH annual meeting.

Objective To investigate the preemptive analgesia therapy of dezocine on postoperative agitation in patients undergoing Esophagectomy under thoracoscopy.Methods Sixty cases of ASA Ⅰ,Ⅱ undergoing thoracoscopic resection of esophageal cancer patients were divided into two groups randomly:dezocine and control group,each group with 30 patients.Patients in dezocine group were intravenously injected with dezocine 0.1 mg/kg before intubation,and patients in control group just intubation as usual.The incidence of postoperative agitation in two groups were observed,and visual analog scale(VAS) score at 0 min,15 min,30 min and 45 min after extubation were registered.The time of wake up and extubatio,the change of hemodynamic were also recorded.Results The incidences of postoperative agitation in dezocine preemptive analgesia group were obviously lower than that in the control group (10％ (3/30) vs.60％ (19/30),P ＜0.01).The VAS score at 0 min,15 min,30 min and 45 min after extubation in dezocine preemptive analgesia group ((1.3 ± 1.2),(2.4 ± 1.7),(2.7 ± 1.3),(2.8 ± 1.2) points) were significantly lower than those in the control group ((3.4±1.5),(4.7±1.8),(5.0±1.6),(4.8±1.7) points) (F within group =18.6,P＜0.05; F between group =20.4,P ＜ 0.05 ; F interaction =20.0,P ＜ 0.05 ; compared different time between two groups,all P ＜ 0.01).Conclusion Preemptive analgesia with dezocine can obviously lessen postoperative agitation in patients undergoing Esophagectomy under thoracoscopy,and dezocine is also effective in postoperative analgesia.

OBJECTIVE: To investigate the effects of Baobaole oral liquid on neuronal excitability in lateral hypothalamic area (LHA) and ventromedial hypothalamic nuclear (VMN) in anorectic rats. METHODS: The anorectic rat model was established by feeding with special prepared forage for a week, and then Baobaole oral liquid, a liquid extract of a compound traditional Chinese medicine for activating spleen, was administered once a day for 3 weeks. Finally, extracellular recording from LHA and VMN neurons in rats were made in order to characterize their responses to gastric vagal nerve stimulation and intravenous injection of glucose in the normal, untreated, and Baobaole-treated groups. RESULTS: There was no statistical difference in response characteristics of LHA neurons to gastric vagal stimulation among 3 groups. The duration of VMN neuron excitation response to gastric vagal nerve stimulation in the untreated group was significantly longer than that of the normal control group (P<0.01), while the required stimulation intensity was significantly decreased (P<0.01). Moreover, among the neurons responding to the gastric vagal stimulation in the untreated group, the number of glycemia-sensitive neurons decreased in LHA and increased in VMN (P<0.01). The gastric vagal stimulation induced neuron responses in LHA and VMN of the Baobaole-treated group were not significantly changed as compared with the normal control group (P<0.01), and neither were the intravenous injection of glucose induced responses. CONCLUSION: Baobaole oral liquid can modulate the sensitivity of LHA and VMN neurons to the peripheral signal and make the coordination between LHA and VMN neurons in order to improve the appetite of anorectic rats.

Objective To investigate the difference of gene expression in two human gastric adenocarcinoma cell lines, 5-fluorouracil resistant cell line SGC7901/R and its parental cell line SGC7901, and to screen related genes of JAK/STAT signaling pathway by gene chip. Methods The mRNAs of two cell lines were extracted and purified. The two cDNA probes were made from these two mRNAs which were labelled by (biotin-)16-dUTP, hybridized with human JAK/STAT signaling pathway gene array and scanned for intensity, respectively. The acquired images were analyzed by software. Different expression genes were then screened out. The mRNA expressions of Stat3, NF-?B1 and bcl-x were analyzed by semi-quantitative RT-PCR, (respectively.) Results A parallel comparison between the gene profiles of JAK/STAT signalling pathway in two cell lines showed that a total of 70 genes were screened out whose expressive level was more than 2 folds in 5-flurouracil resistant cell line SGC7901/R and its parental cell line SGC7901. Among these genes, 40 were upregulated and the other 30 were down-regulated. The results of RT-PCR were consistent with those of microarray scanning. Conclusions The knowledge of gene expression profile of JAK/STAT signaling pathway, which was changed in 5-fluorouracil resistant cell line of human gastric adenocarcinoma, has proven to be useful for illustrating the multi-drug resistance mechanisms of gastric cancer.

Objective To study the effects of matrine in combination with 5-fluorouracil (5-FU) on inhibiting transplanted gastric cancer (cell line SGC-7901) in the nude mice and their myelotoxicity effects. Methods The two dosages of matrine (50 mg/kg and 100 mg/kg) combined respectively with 50 mg/kg of 5-fluorouracil (5-FU) were injected intra-abdominally, and 50 mg/kg or 100 mg/kg matrine or 5-FU injection alone groups served as controls. The relative tumor volume (RTV) and tumor inhibition rate (IR) were calculated. The nude mice bone marrow was taken, the number of the nucleated cells were calculated, and bone marrow colony was cultured. Results The tumor-inhibiting effect in the combined group of 100 mg/kg of matrine +50 mg/kg of 5-FU was significantly increased as compared with those in all the control group ( P

Objective To investigate the effect of inhibiting the expression of Navl. 8 sodium channels by an antisense oligodeoxynudeotide(ODN) on visceral hypersensitivity. Methods The visceral hypersensitivity animal models induced by giving neonatal Sprague-Dawley rats colorectal distention(CRD) after postnatal days 8, 10 and 12. Animals were injected intrathecally either Navl. 8 antisense ODN (research group) or mismatch ODN (control group) twice a day for 3 days at the 8th week. After that, the expression of Navl. 8 in each group was examined by RT-PCR, and abdomiral withdrawal reflex(AWR) score and spinal c-Fos expression were evaluated to assess whether the intervention can relieve the visceral hypersensitivity. Results After intrathecal injection with the antisense ODN, the expression of Navl. 8 in each group decreased.AWR score and the number of c-Fos positive neurons in the spinal cord, especially in the area 1 and 3, decreased in the model group, while they did not change in the control group. Conclusions Interventing the expression of Nav1.8 may relieve the visceral hypersensitivity in this model of irritable bowel syndrome.