Objective:To clone and analyze a full-length cDNA encoding mouse integrin ?7,and repair the mutation of ?7 cDNA that caused the change of amino acids. Methods:The cDNA of ?7 gene was amplified by RT-PCR using the total RNA as extracted from mouse small intestine Peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed E.coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of ?7 cDNA that caused the change of amino acids was repaired. Results:The cDNA of mouse ?7 has an complete open reading frame with a length of 2418 bp,which encodes a product of 806 amino acid,and has 10 base pairs mutation of ?7 gene and 5 base pairs mutation that caused the change of amino acids was repaired. Conclusion:The cDNA of mouse ?7 was cloned successfully,which posed a basis for further researching on its biological function.

Objective To observe and evaluate the clinical curative efficacy of perineal stapled prolapse resection in the treatment of complete rectal prolapse.Methods 15 patients of complete rectal prolapse were all treated by perineal stapled prolapse resection,The anal function of the patients were evaluated according to the Wexner incontinence score standard.Results All patients were successfully completed the operation.Postoperative complications:postoperative bleeding in 2 cases,anastomotic stenosis in 2 cases.These symptoms were relieved after symptomatic treatment.The anal sphincter function was improved in all patients after operation.There were no obvious incontinence.The preoperative Wexner incontinence score was 13.5±1.8,andpostoperative Wexner incontinence score was 4.2±1.5,there were significant statistical difference between them(P<0.05).The mean follow-up were 18 months(2-30 months).There was no recurrence during the follow-up period.Conclusion Perineal stapled prolapse resection as a new type of operation had small trauma,simple operation,less complications and short-term curative effect of good characteristics.They can effectively improve the symptoms of anal incontinence in patients with complete rectal prolapse.

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Objective To observe the effect of lycopene on myocardial interstitial fibrosis and to explore the possible mechanisms involved.Methods Sprague-Dawley rat myocardial infarction (MI) model was established by left anterior descending coronary artery ligation.Established MI model rats received lycopene or saline.After 28 days,myocardial fibrosis was observed by Masson staining.The expressions of Ⅰ type collagen,matvix metalloproteina-ses-9 (MMP-9) and matrix metall opeptidaseg(MAPKs) protein were detected in the peri-infarcted zone by Western blotting.Results In MI group,collagen volume fraction (CVF) was increased and the protein expressions of collagen Ⅰ,P-p38 and MMP-9 were increased in the peri-infarcted zone as compared with the sham group [CVF:(21.68±4.63)％ vs.(8.21±2.17)％; collagen Ⅰ:(1.58±0.22) vs.(0.97±0.09); P-p38:(1.93±0.44) vs.(0.85±0.21); MMP-9:(4.85±0.47) vs.(1.03±0.59); all P＜0.05].Lycopene attenuated the increments of myocardial infarction-induced collagen Ⅰ,p38MAPK and MMP-9 expressions [collagen Ⅰ:(1.24 ± 0.24) vs.(1.58± 0.22) ; P-p38:(0.85 ± 0.21) vs.(1.93 ±0.44); MMP9:(1.77±0.28) vs.(4.85±0.47); all P＜0.05],and decreased the collagen volume fraction in the peri-infarcted zone [CVF:(15.17±2.56)％ vs.(21.68±4.63)％,P＜0.05] as compared with the MI group.Conclusions Lycopene may improve ventricular remodeling after MI by inhibiting p38MAPK signaling pathway and MMP-9 expression.

Objective To study the quality of life(QOL) and its associated factors in patients with traumatic brain injury (TBI) . Methods The 148 TBI patients treated and discharged from department of neurosurgery Chongqing beibei district people′s hospital were selected out and involved in the investigation .the World Health Organization health-related QOL BREF (WHOQOL-BREF) was used to investigate the QOL .116 valid questionnaires were obtained .Results The scores of physiological ,psychological ,social relationship ,environment domains of QOL among the patients with TBI were (69 .37 ± 17 .46) ,(59 .16 ± 14 .47) ,(54 .17 ± 14 .64) , (49 .33 ± 18 .33)scores .Multiple stepwise regression analysis results showed that ,age ,place of residence ,brain injury parting were risk factors for brain injury patient's quality of life ,GOS scores ,degree ,discharge time interval were protect factors (P< 0 .05) . Conclusion The QOL of the patient with TBI discharged from the hospital was lower than the domestic general population .The patient′s age ,residence ,educational level ,the type of TBI ,GOS score ,the time to discharges were the main influencing factors .

Objective To investigate the effect of XueBiJing injection(a concoction of Chinese herbs)on the expression of high mobility group box-1 protein(HMGB1)in the lung with acute lung injury of rats following delayed resuscitation after burn injury.Methods 78 male rats were randomly divided into sham scald group(n=18),scald group(n=30)and XueBiJing treatment group(n=30).In the scald group and XueBiJing treatment group,4ml/kg normal saline or XueBiJing was intravenously injected 2 hours after scald injury.In the latter two groups,rats were subjected to 30% TBSA full-thickness scald injury,and fluid resuscitation was delayed.The animals were sacrificed at 8h,24h and 72h after injury,respectively.Lung tissue samples were collected to determine the expression of HMGB1 mRNA and protein,and the activity of pulmonary myeloperoxidase(MPO).HMGB1 mRNA level was semi-quantitatively measured by RT-PCR using GAPDH as an internal standard,and protein expression of HMGB1 was determined by Western blot.Results Compared with sham scald controls,both mRNA and protein expressions of HMGB1 were significantly enhanced in the lung at 8-72h after scald injury(P

PurposeTo study the pharmacokinetic characteristics of omeprazole in Chinese extensive and poor metabolizers.Methods The pharmacokinetics of omeprazole was studied in eighteen healthy volunteers. After a single oral dose of omeprazole capsule(40 mg), the plasma concentrations of omeprazole and its two metabolites, 5-hydroxyomeprazole and omeprazole sulfone, were determined with reversed HPLC method. The plasma concentration-time data were analyzed to estimate the pharmacokinetic parameters. Results Both plasma omeprazole concentration and the pharmacokinetic parameters exhibited marked interindividual variation. The metabolic ratio (MR= plasma omeprazole/5-hydroxyomeprazole) obtained 3.5 h after medication was used to distinguish between extensive and pcr)r metabolizers (EMs, PMs). The variances of AUC(0-12) caused by the two metabolizer phenotypes accounted for 75.4 % of the total interindividual variances. AUC(0-12) of omeprazole was (1 971.78 ±1 221.78)ng·h/ml in EMs( n=12) and (8 587.18±2 855.48) ng·h/ml in PMs (n = 6),respectively (P<0.01),and CL in EMs and PMs was estimated as (16.00±9.71) and (4.79±1.32) L/h (P<0.01). Accordingly,significantly lower level of plasma 5-hyclroxyomeprazole was found in PMs, revealing a slower hydroxylation rate compared with EMs. Conclusions The results suggest that individualized dose regimen of omeprazole, based on identification of metabolizer phenotype, can be of great benefit from the viewpoint of pharmacoeconomics.

High-mobility-group B1 (HMGB1), an abundant, highly conserved cellular protein, is widely known as a nuclear DNA-binding protein that stabilizes nucleosome formation, and facilitates gene transcription. Recent studies suggested that HMGB1 could be overexpressed and released from cellular nucleosome upon endotoxin and cytokine stimulation, or other stress challenge including burns, shock, as well as infection. Therefore, extracellular HMGB1 might be involved in the pathogenesis of sepsis and subsequent multiple organ dysfunction syndrome. Moreover, experimental data showed that extracellular HMGB1 might play vital roles in nerves development, tumor metastasis, atherosclerosis and restenosis after vascular damage.

Objective To summarize countermeasures on common problems of microendoscopic discectomy(MED). Methods Microendoscopic discectomy was performed in 104 cases of lumbar disc herniation by using the MED system(Sofamor Danek Group,USA).Frequently encountered problems during the operation were reviewed and summarized. Results Conversions to open surgery were required in 4 out of 104 cases due to bleeding or adherence.Among the rest of 100 cases,dural injury occurred in 3 cases,with 1 case accompanying leakage of cerebrospinal fluid.Of the 100 cases(115 intervertebral spaces),the average operation time was 50 min(range,30~90 min) and the average hemorrhage amount was 80 ml(range,20~400 ml) for each intervertebral space.The 100 cases were followed for 3~32 months(average,18 months).According to the Macnab criteria,the curative effects were classified as excellent in 65 cases,good in 29 cases,and fair in 6,the rate of excellent or good effects being 94.0%(94/100). Conclusions The most frequent problems during MED are bleeding and prolonged excision of the ligamenta flava.Strict adherence to technique,acquaintance with the weakness of the ligamenta flava,and familiarity with anatomic structures of the vertebral vein system and its relationship with abdominal pressure,are very important in the prevention and treatments of surgical complications.

Objective To explore whether or not the cartilage damage in the pathogenesis of pathologic synovial plicae is due to the matrix metalloproteinase. Methods The immunohistochemical method was used to detect the expression and distribution of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in arthroscopically clarified pathologic synovial plicae and normal synovial plicae of the knee joint.Results The positive expression rate of MMP-1 and TIMP-1 had significant differences between the pathologic and normal synovial plicae(?~2=16.014,P=0.000;?~2=4.059,P=0.044).The expression of MMP-1 was positive in the synovial lining cells,monocytes,lymphocytes,endothelial cells,and chondrocytes,but negative in the normal synovial plicae.The TIMP-1 expression was only detected in the synovial lining cells and a small quantity of fibroblast cells.The immunohistochemical analysis revealed a greater number of positive cells and intensity of staining of MMP-1 than TIMP-1.Conclusions The development of pathologic synovial plicae may yield MMP-1 and TIMP-1 with unbalanced distributions,which may be the biological basis of the pathogenesis of cartilage destruction.