Objective To examine the effect of mycophenolic acid(MPA)on mesangial cell(MC)proliferation and on expressions of transforming growth factor-β(TGF-β)and connective tissue growth factor(CTGF)induced by high glucose.Methods The proliferation activity of mesangial cells incubated for 24h,48h and 72h in different concentrations of MPA was observed by MTT assay.The expressions of TGF-β and CTGF were measured by Western blot.Results MPA inhibited the MC proliferation and expressions of TGF-β and CTGF induced by high glucose.Conclusions MPA decreases the release of extracellular matrix by inhibiting the MC proliferation and expressions of TGF-β and CTGF,and subsequently slows the development of glomerulosclerosis in diabetic nephropathy.

Objective To examine the effect of mycophenolic acid(MPA)on mesangial cell(MC)proliferation and on expressions of transforming growth factor-?(TGF-?)and connective tissue growth factor(CTGF)induced by high glucose.Methods The proliferation activity of mesangial cells incubated for 24h,48h and 72h in different concentrations of MPA was observed by MTT assay.The expressions of TGF-? and CTGF were measured by Western blot.Results MPA inhibited the MC proliferation and expressions of TGF-? and CTGF induced by high glucose.Conclusions MPA decreases the release of extracellular matrix by inhibiting the MC proliferation and expressions of TGF-? and CTGF,and subsequently slows the development of glomerulosclerosis in diabetic nephropathy.

OBJECTIVE:To observe and probe into the effects and mechanism of action of calcium dobesilate on the con?tents of vasoactive substances in the remnant kidneys of rats with chronic renal failure.METHODS:The rats were randomly divided into sham operation,model and calcium dobesilate therapy group.Except the sham operation group,the rest two groups underwent nephrectomy and were established as chronic renal failure models.One week later either drink water or calcium dobesilate were irrigated into the stomachs of the rats in all three groups.And the effects of calcium dobesilate on renal function,contents of part vasoactive substances and the renal pathological changes of remnant kidney were examined.RESULTS:In calcium dobesilate therapy group,urine protein decreased,and the renal function improved,as compared with the model group.The contents of Thromboxane A 2 in the renal cortex significantly decreased and the pathological changes of remnant kidney lessen significantly.CONCLUSIONS:Calcium dobesilate may protect kidney by decreasing the content of Thromboxane A 2 in the remnant kidney.