Objective To research the status of health literacy among a vocational high school in Guizhou province ,and provide the evidence for health education for the students in this school .Methods Independent design a health literacy questionnaire based on Chinese citizens′health literacy questionnaire in 2008 ,and random sampling 380 students from freshman to senior of this school to investigation in November ,2012 .Results 78 .35% of the students in this school had a basic health knowledge ,39 .57% students had a healthy lifestyle and behaviors ,and 66 .61% students had a sense of the basic literacy skills .The difference was statistically significant among the three items(F=651 .710 ,P<0 .01) .From the overall condition of the health literacy ,the students form high-er household income have higher health quotient ,but with no statistically significant (P>0 .05) ,meanwhile ,there was no difference in health quotient on household registration and gender students .With the increase of school age ,student health literacy level was reduced ,the difference was statistically significant (F=3 .657 ,P<0 .05) .Conclusion The health literacy of lifestyle and behavior of students in this school is poor .Education should be targeted to develop healthy habits .As for the junior and senior students ,the school should enhance the health education intensity to improve the health literacy .

Objective To evaluate the effect of an equivalent uiform dose optimization (EUD) based optimization algorithm in sparing the organ at risk (OAR) of tumor treated with intensity-modulated radiation therapy (IMRT).Methods 10 patients of nasopharyngeal carcinoma (NPC),10 patients of esophagus cancer,6 patients of rectum cancer were randomly selected from all patients received IMRT treatment.Then physical optimization constraints (dose/dose-volume constraints) were designed as EUD optimization,while the physical objectives for targets and other OAR were unchanged.Results There was statistically significant difference in protecting OAR in 8 NPC patients of PLANED group compared with those of PLANNO_EuD group (t values were 2.590,2.352,3.656,2.148,2.283,3.683,2.192,2.353,2.146,2.276,2.126,P ＜ 0.05).There was no statistically significant difference in protecting OAR in 10 cases of esophageal cancer between PLANEUD group and PLANNO_EUD group (t value were 0.408,0.427,0.920,P ＞ 0.05).There was statistically significant difference in protecting OAR in 6 cases of rectum cancer of PLANED group compared with those of PLANNO_EUD group (t values were 2.332,2.693,2.279,2.244,P ＜ 0.05).Conclusion EUD optimization can reduce the doses to OAR which may be quite helpful to reduce the occurrence rate of OAR complications.

Objective:To explore psychosocial factors of depressed elderly patients. Methods:33 elderly people with the first episode of depression diagnosed according to CCMD-3, and elderly healthy people were investigated with EPQ, SSRS (social support rating scale), LES (life event scale) and MMSE (mini mental state examination). Results:The rates of psychiatric family history, somatic diseases, risk factors for vascular disease and long-term difficulties were significantly higher in the depressive group than in control group. The score of MMSE was significantly lower in depressive group than in control group. The scores of negative life events and problems in social intercourse were significantly higher in depressive group than in control group. There were lower score of social support and its utility in the depressive group than in control group. The score of neuroticism was significantly higher in the depressive group. Most depressive elderly patients had the personality of neuroticism.Conclusion:Chronic stress, high neuroticism and severe cognitive impairment are important risk factors for depression in late life.[

Objective:Measured the soluble forms of intercellular adhesive molecule 1 (sICAM 1), soluble vascular cell adhesion molecule ( sVCAM 1) and interleukin 1(IL 1),tumor necrosis factor(TNF), interferon ?(IFN ?),study the role of sICAM 1 and sVCAM 1 in rheumatoid arthritis(RA).Methods:A sandwich ELISA technique was used to measure the serums levels of sICAM 1, sVCAM 1 ,IL 1,TNF,IFN ? in 30 patients with active RA and 30 normal control subjects.Results:The levels of sICAM 1 and sVCAM 1 in patients with RA were significantly higher than that in control subjects(P

Objective To study the Effect of Liuwei anxiao capsules combined with omeprazole for the gastric electrical parameters and gastrointestinal motility related indexes of patients with functional dyspepsia. Methods 86 patients with functional dyspepsia in our hospital from December 2014 to July 2016 were selected,and all the patients were randomly divided into control group and observation group,43 cases in each group,the control group were treated with omeprazole,the observation group were treated with Liuwei anxiao capsules on the basis of the control group,then the total effective rates of different symptoms,signs,gastric electrical parameters and gastrointestinal motility related indexes before and after the treatment of two groups were compared. Results The total effective rates of observation group with abdominal pain,abdominal distension,early satiety and belching were all higher than those of control group (P<0.05).3,7 and 14 days after treatment,compared with the pre-treatment, the difference of gastric electrical parameters between the two groups was statistically significant (P<0.05),the ratio of normal slow rhythm rhythm, main electrogastric power and electrogastric frequency in observation group were higher than those in control group (P<0.05),the percentage of bradycardia was lower than that in control group (P<0.05);there were significant differences in the indexes of gastrointestinal motility between the two groups before treatment (P<0.05),the level of MTL before and after meals was higher in the observation group than that in the control group (P<0.05), and the levels of SS before and after meals were lower than those in the control group (P<0.05). Conclusion Liuwei anxiao capsules combined with omeprazole in the treatment of functional dyspepsia in patients with significant effect,can significantly improve the patient's gastric electrical parameters and gastrointestinal motility related indicators,and improve the clinical symptoms.

Long terms of chronic pain may induce emotional disorder such as depression,anxiety and aversion.It is worthy of studying the mechanism and therapy on pain emotional disorder.The animal model is important in basic researches,and the quantitative evaluation methods on the psychiatric factors are gradually applied in the pain research.The methods of evaluating the psychiatric disorder of pain are discussed in this paper,which will provide theory basis in the relevant fields.

Objective To improve the ethics review process and ethical standards,strengthen the implantation of ethical review of research involving human subject in our hospital.Methods Evaluating the review practice of research involving human subject in our hospital practices,learn and bring in the latest international experiences.Results As China's Ethical Committee is in its initial stage,the regulatory ability is still in defective and insufficient.A healthy and operable ethic review mechanism needs to be perfected gradually along with the practice.Conclusions In the aspect of improving the ethical review work of human subject research,some new methods and ideas were discussed.In addition to strengthen the current organizational system,we should clarify the elements of the review of different types of research and carry out multi level ethical trainings.The establishment of a unified,specific evaluative system,combined with this information platform is continuously monitoring the clinical study.This will ensure the human research projects comply with ethical requirements,and further promote the sustainable development of clinical research.

Objective:To explore the inflammatory responses of macrophages treated with Helicobacter pylori. Methods:Cytokines IL-23,IL-10,TNF-α and IL-8 in cell culture supernatant of macrophages stimulated with Helicobacter pylori were determined by ELISA kits,and the expression of intracellular proteins NOS2 and COX2 in Helicobacter pylori treated macrophages was analyzed by Western blot. Then,the apoptosis of Helicobacter pylori stimulated macrophages was detected by flow cytometry. Results:The secretion of cytokines IL-23,IL-10,TNF-α and IL-8 in the culture supernatant of Helicobacter pylori treated macrophages increased significantly (P<0. 05),and the expression of NOS2 and COX2 was enhanced evidently(P<0. 05). Meanwhile,helicobacter pylori could induce the apoptosis of macrophages obviously ( P<0. 03 ) . Conclusion: The inflammatory responses of macrophages treated with Helicobacter pylori would be promoted to inhibit or kill Helicobacter pylori,furthermore,Helicobacter pylori could induce the apotosis of macropha-ges.

Objective To evaluate the value of serum procalcitonin (PCT ) in the diagnosis of liver cirrhosis complicating sponta‐neous bacterial peritonitis (SBP) .Methods The patients with cirrhosis were divided into non‐SBP group and complicating SBP group according to whether complicating SBP ,and the patients with common hepatitis served as the control group .Serum expres‐sion levels of PCT ,CRP and IL‐6 were detected by electrocheniluminescence and Immunoturbidimetry methods .The receiver operat‐ing characteristic curve (ROC) curve was drawn for evaluating the diagnostic efficiency of each indicator .Results The levels of ser‐um PCT ,CRP and IL‐6 in the complicating SBP group were significantly higher than those in the non‐SBP group and control group , the differences were statistically significant (P<0 .01);The ROC curve analysis showed that the diagnostic value of PCT at the op‐timum threshold value of 0 .51 ng/mL for diagnosing cirrhosis complicating SBP was superior to CRP and IL‐6 ,the sensitivity of se‐rum PCT for diagnosing SBP in 3 groups was 62 .68% ,the specificity was 76 .59% and the accuracy was 80 .01% .Conclusion The serum PCT level has an important value for the early diagnosis of liver cirrhosis complicating SBP .

AIM: To study the expression of c-jun in brain stem following moderate lateral fluid percussion brain injury in rats, and to observe the temporal patterns of its expressions following percussion.METHODS: Male Sprague-Dawley rats were divided into normal control, sham operation control and injury groups. The rats of injury group subjected to moderate lateral fluid percussion injury (0.2 mPa), and then were subdivided into 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 8 h and 12 h groups according to the time elapsed after injury. The expression of c-jun was studied by immunohistochemistry and in situ hybridization. RESULTS: After percussion for 15 min, Jun positive neurons increased in brain stem progressively, and peaked at 12h. At 5min after percussion, the induction of c-jun mRNA was increased, and remained elevated up to 1h-2h after brain injury. CONCLUSION: The induction and expression of the c-jun in brain stem after fluid percussion brain injury were increased rapidly and lasted for a long time.