Objective To observe the protective effect of grape seed proanthocyanidin extract ( GSPE) on learning disabilities after radiation injury brain in rats.Methods 120 male Wistar rats were divided into control group,model group,high and low dose GSPE groups.The radiation injury brain models were established using a method of linear accelerator irradiation in 22 Gy.The morphological changes of neu-rons in hippocampus were observed with HE staining; the expression of phosphorylated extracellular signal regulated kinase 1/2 ( ERK1/2) and growth associated protein-43( GAP-43) were detected by immunohisto-chemistry;the contents of malonalde hyde(MDA) and superoxide dismutase(SOD) were measured respec-tively by thiobarbituric acid( TBA) and xanthine oxidation( XTO) methods;the learning ability was assessed with shuttle box.Results Compared with the model group,the GSPE groups showed a decreased degree of nerve cell morphological injury; and increased ERK1/2 activities((13.20±1.45)/view,(27.40±2.52)/view,(19.80±1.30)/view),GAP-43 expression level((52.93±2.07)/view,(66.50±0.77)/view,(73.05± 2.40)/view) and SOD activities((79.82±5.26) U/mg,(76.20±6.86) U/mg,(80.12±5.76) U/mg)(P<0.05);and decreased the contents of MDA((71.62±1.88) μmol/g,(76.41±1.94) μmol/g,(72.32±1.98)μmol/g)(P<0.05).Shuttle box testing showed that the active avoidance reaction rate was increased(7 d:(56.23±2.56)%;14 d:(44.66±2.79)%;28 d:(50.40±2.16)%)(P<0.05)and passive avoidance latency was shorted(7 d:(34.11±1.57)s;14 d:(45.52±1.88)s;28 d:( 39.52±1.79)s)(P<0.05) in GSPE groups. The above mentioned changes were more significant in high dose of GSPE(P<0.05) .Conclusion GSPE has protective effect on learning disabilities of radiation injuries brain,which is related to enhance ERK1/2 activ-ity and the expression of GAP-43.

Purpose:To study the role of postoperative radiotherapy in early stage uterine sarcomas. Methods:Retrospective analysis of the impact of radiotherapy on progress-free survival rate (PRS )rate, local recurrence rate (LRR) and overall survival (OS)in stage Ⅰ,Ⅱ uterine sarcomas. 34 cases were treated with surgery alone ,42 cases were treated with postoperative radiotherapy. Results:The PRS at 2 and 5 years for surgery were 38.2% and 26.5% ,69.0% and 57.1%for postopeative radiotherapy. The LRR at 2 and 5 years treated with surgery were 52.9% and 64.7%,26.2% and 28.6% with adjuvant irradiation. The OS at 2 and 5 years treated with surgery were 83.4%and 47.0%,83.3% and 69.0% with adjuvant radiation. Conclusions:Postoperative radiotherapy showed a marked increase in the three parameters studied (PRS? LRR?OS ) for I,II stage uterine sarcomas.

Objective To observe the effect of grape seed proanthocyanidin extract (GSPE) on growth associated protein-43 (GAP-43) through extracellular signal regulated kinase 1/2 (ERK1/2) pathway in rats with brain radiation injury. Methods 72 3-month male Sprague-Dawley rats were divided into control group (n=18), model group (n=18), high dose GSPE group (n=18) and low dose GSPE group (n=18). The brain radiation injury models were established by linear accelerator irradiation with 22 Gy. The learning ability was assessed with shuttle box. The morphological changes of neurons in hippocampus were observed with HE staining;the expression of GAP-43 was de-tected by RT-PCR; and the expression of phosphorylated ERK1/2 was detected by Western blotting. Results Compared with the model group, the active avoidance reaction rate in the shuttle box test increased and the passive avoidance latency shortened in GSPE groups (P<0.001); the nerve cell morphological injury reduced and the expression of GAP-43 mRNA and phosphorylated ERK1/2 increased in the GSPE groups (P<0.001), especially in the high dose GSPE group (P<0.001). The GAP-43 mRNA level positively correlated with phosphory-lated ERK1/2 level in the model group (r=0.764, P<0.001), the low dose GSPE group (r=0.814, P<0.001) and the high dose GSPE group (r=0.822, P<0.001). Conclusion GSPE could promote the expression of GAP-43 through ERK1/2 pathway in rats, and prevent the brain from radiation injury.

Purpose:To study the adjuvant therapy in uterine sarcomas,to decrease local recurrence and metastases and improve survival.Methods:Analysis of the management of 113 cases of uterine sarccoma , retrospectively. 30 cases were treated weith surgery alone , 25 with adjuvance radiation , 34 with postoperative chemotherapy, 21 with surgery and chemo radiation.Results:The 2 year pelvic recurrence and metastases with surgery alone were 43.3 % and 30 0 %,32 0 % and 28 0 % with postoperative radiation,44.1 % and 20.6 % with postoperative chemotherapy, 23.8 % and 23.8 % with postoperative combination of chemo radiation.But there was no significant difference between surgery alone and postoperative adjuvant therapy.Conclusions:Postoperative radiotherapy can improve local control,adjuvant chemotherapy can decrease metastases,both can improve disease free survival,but can not improve overall survival for uterine sarcomas.

A high performance liquid chromatography-tandem mass spectrometric method ( HPLC-MS/MS ) was developed for the simultaneous determination of 31 kinds of triazine herbicides in farmland soils. Samples were extracted by accelerated solvent extraction ( ASE ) and purified by Oasis MCX solid phase extraction ( SPE) cartridges, and then analyzed by HPLC-MS/MS in multiple reaction monitoring ( MRM) mode with positive electrospray ionization. The analytical column was Phenomenex Luna C18 ( 150 mm í 2. 0 mm í3 μm) and the mobile phases were acetonitrile and water containing 0. 1% (V/V) formic acid. The limits of detection (S/N≥3) were 0. 008-0. 440 μg/L. All of the triazine herbicides had good linear responses with r≥0 . 996 and the average recoveries in the spiked levels of 0 . 40-40 . 0 μg/kg ranged from 76 . 9%-102 . 0%with the RSDs of 3 . 4%-10 . 3%. The HPLC-MS/MS method had been applied for the detection of the triazine herbicide residues in farmland soils from Shenyang region and the results showed that atrazine, simazine, prometryne and atrazine-desethyl were the main triazine herbicides in the region.

OBJECTIVE To study the level of plasma fibrinogen in patients with common facial paralysis, and investigate the relationship between the plasma fibrinogen and the pathogenesis of facial paralysis.METHODS A total of 157 inpatients with different kinds of facial paralysis were studied from December 2006 to December 2008.They were etiologically divided into five groups:severe Bell's palsy group(60 cases), moderate Bell's palsy group(19 cases), Ramsay-Hunt syndrome group(28 cases), temporal bone fracture with facial palsy(28 cases), and facial nerve tumor group(22 cases).They were tested for plasma fibrinogen level right after admitted to hospital.RESULTS Plasma fibrinogen level in severe Bell's palsy group was(4.08?0.90)g/L, which was significantly higher than that in other groups and the normal value(2.0～4.0)g/L(P

Growth hormone (GHRH) and pitutary adenylate cyclase activating peptide (PACAP) are the members of the PACAP/Glucagon superfamily,who are related in both sequence and function.Their stimulation of GH secretion and animal growth is concerned.A series of expression plasmid,pIRES1-GHRH-PACAP (P-G-P),plRESI-GHRH (P-G) and plRESI-PACAP(P-P),were constructed,extracted and purified,then transfected into CHO cell line with Lipofectamine.The expression was examined by RT-PCR,dot-ELISA and Western blotting.The biological activity of expression products was detected in rats.At 8 h after injection of transfection supematant,serum IGF-I concentrations in P-G-P group were significantly higher than that in other groups(P ＜ 0.05).PLGA encapsulating plasmid microspheres were prepared and injected intramuscularly into rabbit legs.Growth behavior and IGF-1 level were measured at day 0,15,30 and 45 after injection.Greater body weights gain and higher serum 1GF- [ levels were observed in three plasmid microsphere injection groups,compared with control group.At day 30,the body weight gain in P-G-P group was greater than saline group (81%,P＜ 0.01),P-G mierosphere group (15%,P＜ 0.05) and P-P microsphere group (7%,P＞ 0.05),serum IGF-I concentration in P-G-P microsphere group showed a 16.68% increase to P-G microsphere (P ＞ 0.05),a 17.14% increase to P-P microsphere(P ＞ 0.05) and a 50.46% increase to control (P ＜ 0.05).These results suggest that co-expression of GHRH and PACAP in one expression plasmid might exert an additive stimulation of GH secretion and growth when delivered into rabbit skeletal muscle with PLGA mierosphere.The results may provide a new approach to regulate animal growth.

Objective To investigate the molecular and cell signal transduction mechanisms by Lactobacillus cell wall extract(LCWE)inducing human-β-defensin-2(hBD-2)expression in human vaginal epithelial cells.Methods The induction of hBD-2 in human vaginal epithelial cells(WZV-1)by LCWE was observed using real-time PCR and Western blot.After stimulating WZV-1.the activation of NF-κB and p38MAPK signaling pathways were determined by Western blot.The induction of hBD-2 in WZV-1 cells by LCWE was observed with signaling pathways inhibitors of NF-κB and p38MAPK using real-time PCR and Western blot.Results The results showed that LCWE significantly upregulated hBD-2 expression in the time and dose-dependent manner.The maximal stimulatory effect of LCWE on the expression of hBD-2mRNA in WZV-1 cells were observed at the concentration of 50μg/ml after treatment for 8 h.After stimulation by 50μg/ml LCWE,Western blot analysis demonstrated that the phosphorylation of p38MAPK increased at 0.5 h significantly,peaked at 1 h,moreover the concentration of NF-κB in nucleus increased at 0.5 h significantly(P<0.05),peaked at 2 h.Blocking with inhibitor of NF-κB and(or)p38MAPK pathways results in decreased levels of HBD-2 expression.Conclusion These findings suggest that p38MAPK and NF-κB pathways play the important roles in induction of hBD-2 expression by LCWE in human vagihal epithelial cells.

Objective To investigate the effects of butylphthalide on brain edema,blood-brain barrier permeability and RhoA expression in cortex in focal cerebral infarction in rats.Methods A total of 220 male Sprague Dawley rats were divided into a control,a model and a butylphthalile group (40 mg/kg,once a day,gavage) according to the random number table method.A model of rat focal cerebral infarction was induced by photochemical method.At 3,12,24,72,and 144 h after modeling,wet-dry weight method and Evans blue extravasation method were used to detect the brain water content and blood-brain barrier permeability.At 24 h after modeling,immunohistochemical staining and Western blot were used to detect the expression of RhoA protein in the periinfarction cortex.Results Compared to the control group,the brain water content (except at 6 h) and blood-brain barrier permeability in the model group and the butylphthalide group were increased significantly (all P ＜ 0.05).The immunohistochemical staining and Western blot suggested that the RhoA expression in the periinfarction cortex was upregulated significantly (all P ＜ 0.05).Compared to the model group,the brain water content and blood-brain barrier permeability at different time points in the butylphthalide group were decreased significantly (all P ＜ 0.05).The expression levels of RhoA were also decreased significantly (P ＜ 0.05).Conclusions Butylphthalide may reduce the brain edema of focal cerebral infarction in rats,inhibit disruption of the blood-brain barrier,and down-regulate the expression of RhoA.

Objective To investigate the effect of butylphthalide on brain edema and the expression of phosphorylated myosin light chain in peri-infarct tissue in focal cerebral ischemic rats.Methods A total of 212 adult male Sprague-Dawley rats were divided into a sham operation group (n =12),a cerebral ischemia group (n =100),and a butylphthalide group (n =100) (40 mg/kg,1/day,gavage) according to the random number table.Both the cerebral ischemia group and the butylphthalide group were redivided into 6 h,12 h,1 d,3 d,and 7 d groups (n =20 for each time point).A rat model of focal cerebral ischemic model was induced by photochemical method; 2,3,5-triphenyl tetrazolium chloride (TTC) staining was used to detect infarct volume (n =5); wet-dry weight method was used to detect the water content in brain tissue (n =5);immunohistochemistry (n =5) and Western blot (n =5) were used to detect the protein expression of periinfarct cortical p-MLC.Results TTC staining showed that no infarcts were observed in the sham operation group.The infarct volumes at each time points in the butylphthalide group were significantly less than those at the same time points in the cerebral ischemia group (all P ＜0.05).Wet-dry weight method showed that the water contents in brain tissue in the cerebral ischemia group and the butylphthalide group were significantly higher than that in the sham operation group (all P ＜ 0.05),but the water contents in brain tissue at each time points in the butylphthalide group was significantly lower than those at the same time points in the cerebral ischemia group (all P ＜ 0.05).Both immunohistochemistry and Western blot showed that the expressions of peri-infarct cortical p-MLC in both the cerebral ischemia group and the butylphthalide group were upregulated significantly compared to the sham operation group (all P＜ 0.05),but the expressions of peri-infarct cortical p-MLC at each time points in the butylphthalide group was significantly lower than those at the same time points in the cerebral ischemia group (all P＜ 0.05).Conclusions Butylphthalide can be up-regulated by reducing the expression of p-MLC caused by cerebral ischemia and reduce cerebral edema,and then reduce the infarct volume,and thus play a neuroprotective effect.